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Showing: 8001 - 8050 of 10038 results

  3. pPicZ:mTREK-1_cryst_I110D

    Plasmid
    #209640
    Purpose
    P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322, I110D) fused to a C-terminal GFP
    Depositor
    Dan Minor
    Article
    Pope et al Cell Chem Biol. 2020 May 21;27(5):511-52
    Insert
    Potassium channel subfamily K member 2 (KCNK2, TREK-1) I110D mutant
    Tags
    SNS- 3C_cleavage_site-TAAA-GFP
    Expression
    Yeast
    Mutation
    aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…
    Available Since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    209640-plasmid-map-sequence-id-417096

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  5. AA125

    Plasmid
    #215982
    Purpose
    Fragmid fragment: (N' terminus) activator from p65 subunit of mouse Nfkb
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    Start; p65_v1.3; HSF1_v1.2; Linker_v3.1; SV40NLS_v1.6
    Use
    Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215982-plasmid-map-sequence-id-431448

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  6. PCUP1-GFP-Osh1-406

    Plasmid
    #207007
    Purpose
    Integration of Osh1-GFP as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Yeast PMN pathway substrate.
    Depositor
    Zhiping Xie
    Article
    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.
    Insert
    OSH1
    Tags
    GFP
    Expression
    Yeast
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    207007-plasmid-map-sequence-id-422254

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  10. pGG-GTS-TurboID-GFP

    Plasmid
    #209405
    Purpose
    Transiently expressing and visualizing the subcellular localization of TurboID fused with a Golgi targeting sequence (GTS) in planta
    Depositor
    Rosa Lozano-Durán
    Article
    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1
    Insert
    GTS-3XHA-TurboID
    Tags
    GFP
    Expression
    Plant
    Mutation
    TurboID gene sequence C249A
    Promoter
    Cauliflower mosaic virus (CaMV) 35S promoter
    Available Since
    Jan. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    209405-plasmid-map-sequence-id-418457

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  11. pRH3175

    Plasmid
    #210177
    Purpose
    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1
    Depositor
    Randy Hampton
    Article
    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.
    Insert
    YIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1 (ARO7 Budding Yeast)
    Expression
    Yeast
    Mutation
    R33G,G141S
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    210177-plasmid-map-sequence-id-415795

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  14. pHR-LS

    Plasmid
    #205438
    Purpose
    Expression of Gaussia luciferase (gLuc) and spectinomycin resistance (Spec) genes
    Depositor
    Masamichi Kamihira
    Article
    Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478
    Inserts
    Gaussia luciferase gene
    spectinomycin resistance gene
    Use
    Cre/Lox and Luciferase; Chlamydomonas expression
    Promoter
    Heat shock protein 70A (HSP70A)/ribulose bisphosp…
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    205438-plasmid-map-sequence-id-405460

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  15. pGBT9-GGA2-GAE

    Plasmid
    #199175
    Purpose
    Expression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.
    Insert
    GGA2-GAE domain (GGA2 Human)
    Tags
    GAL4-DNA binding domain fragment
    Expression
    Yeast
    Mutation
    Contains Lys 545 Gln substitution
    Promoter
    ADH1
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199175-plasmid-map-sequence-id-389860

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  16. iEscSnFR

    Plasmid
    #182807
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do
    Insert
    iEscSnFR
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    182807-plasmid-map-sequence-id-358170

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  17. iFluoxSnFR

    Plasmid
    #182808
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do
    Insert
    iFluoxSnFR
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    182808-plasmid-map-sequence-id-358173

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  21. pDD2604

    Plasmid
    #175088
    Purpose
    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T604A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).
    Depositor
    Dirk Dittmer
    Article
    Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi
    Insert
    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    Expression
    Mammalian
    Mutation
    S644A
    Available Since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    175088-plasmid-map-sequence-id-345873

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  22. pDD2602

    Plasmid
    #175086
    Purpose
    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T424A, T438A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).
    Depositor
    Dirk Dittmer
    Article
    Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi
    Insert
    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    Expression
    Mammalian
    Mutation
    T424A, T438A
    Available Since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    175086-plasmid-map-sequence-id-345870

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  23. pDD2603

    Plasmid
    #175087
    Purpose
    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T504A, T508A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).
    Depositor
    Dirk Dittmer
    Article
    Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi
    Insert
    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    Expression
    Mammalian
    Mutation
    T504A, T508A
    Available Since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    175087-plasmid-map-sequence-id-345871

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  24. pN1mcherry-NKD2EFMUT

    Plasmid
    #174333
    Purpose
    encodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.
    Depositor
    Sonal Srikanth
    Article
    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:
    Insert
    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    Tags
    mCherry tag
    Expression
    Mammalian
    Mutation
    NKD2132DFD134>AFA (substitutions of D132 and D…
    Promoter
    CMV
    Available Since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174333-plasmid-map-sequence-id-349872

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  27. EGFP-acVHH (Y34W)-PB

    Plasmid
    #171072
    Purpose
    A COSMO mutant (Y34W) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody variant
    Tags
    modified STIM1–PB tag
    Expression
    Mammalian
    Mutation
    Y34W
    Promoter
    CMV
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171072-plasmid-map-sequence-id-338143

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  28. EGFP-acVHH (Y34F)-PB

    Plasmid
    #171071
    Purpose
    A COSMO mutant (Y34F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody variant
    Tags
    modified STIM1–PB tag
    Expression
    Mammalian
    Mutation
    Y34F
    Promoter
    CMV
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171071-plasmid-map-sequence-id-338141

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  29. EGFP–acVHH (Y104F)–PB

    Plasmid
    #169683
    Purpose
    A COSMO mutant (Y104F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody variant
    Tags
    modified STIM1–PB tag
    Expression
    Mammalian
    Mutation
    Y104F
    Promoter
    CMV
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    169683-plasmid-map-sequence-id-338137

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  30. pBbB8k-csg-NbSARS

    Plasmid
    #171703
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA is fused to a cross-reactive nanobody targeting the spike protein of betacoronaviruses via a flexible linker.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1
    Insert
    csgBACEFG, with nanobody fused to CsgA
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171703-plasmid-map-sequence-id-336471

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  33. iSKetSnFR2

    Plasmid
    #140467
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for S-ketamine, termed iSKetSnFR2, thus enabling optical subcellular pharmacokinetics for S-Ketamine.
    Depositor
    Henry Lester
    Article
    Bera et al Front Cell Neurosci. 2019 Nov 12;13:499.
    Insert
    iSKetSnFR2
    Tags
    His Tag, HA Tag and Myc tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    140467-plasmid-map-sequence-id-296110

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  34. iSKetSnFR1

    Plasmid
    #140466
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for S-ketamine, termed iSKetSnFR1, thus enabling optical subcellular pharmacokinetics for S-Ketamine.
    Depositor
    Henry Lester
    Article
    Bera et al Front Cell Neurosci. 2019 Nov 12;13:499.
    Insert
    iSKetSnFR1
    Tags
    His Tag, HA Tag and Myc tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    140466-plasmid-map-sequence-id-296106

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  37. pCIG1_UL136_19kDa_EE

    Plasmid
    #74951
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    Glu-Glu tag (EYMPME)
    Expression
    Mammalian
    Mutation
    delta 1-127
    Promoter
    CMV, lentiviral LTR
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    74951-plasmid-map-sequence-id-218061

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  38. pCIG1_UL136_23kDa_Myc

    Plasmid
    #74949
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    HA Tag (YPYDVPDYA)
    Expression
    Mammalian
    Mutation
    delta 1-99 M128A
    Promoter
    CMV, Lentiviral LTR
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    74949-plasmid-map-sequence-id-218062

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  39. CoxVIIIx2-CeNL(Ca2+)_1.2μ-pcDNA3

    Plasmid
    #111922
    Purpose
    Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2μ
    Depositor
    Takeharu Nagai
    Article
    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/
    Insert
    CeNL(Ca2+)_1.2μ
    Tags
    CoxVIIIx2
    Expression
    Mammalian
    Mutation
    E67D, E104D, D133E at CaM
    Promoter
    CMV
    Available Since
    June 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    111922-plasmid-map-sequence-id-214013

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  41. pAM5081

    Plasmid
    #85107
    Purpose
    Ptrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels.
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    YFP-KaiC
    Tags
    YFP
    Expression
    Bacterial
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85107-plasmid-map-sequence-id-180104

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  44. pCIG1_UL136_25kDa_Myc

    Plasmid
    #74954
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)
    Expression
    Mammalian
    Mutation
    delta 1-77, M100A M128A
    Promoter
    CMV, Lentiviral LTR
    Available Since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74954-plasmid-map-sequence-id-141774

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Showing: 8001 - 8050 of 10038 results