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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,227 results for: Vars

Showing: 3201 - 3220 of 23227 results

  1. pETM33_Nsp15

    Plasmid
    #156481
    Purpose
    Bacterial expression of Sars-CoV2 Nsp15 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp15 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156481-plasmid-map-sequence-id-307733

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  2. pCKC775

    Plasmid
    #226626
    Purpose
    piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, sfGFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    7xTCF-LEF and EF1a
    Available Since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226626-plasmid-map-sequence-id-449161

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  3. pCKC775

    Plasmid
    #226626
    Purpose
    piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT S. pyogenes, Human)
    EF1a promoter, sfGFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    7xTCF-LEF and EF1a
    Available Since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226626-plasmid-map-sequence-id-449161

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  4. pCKC764

    Plasmid
    #226625
    Purpose
    piggyBAC pCAG-hCas9-T2A-Unbiased3
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Human, S. pyogenes, Chicken)
    EF1a promoter, blasticidin resistance
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV and EF1a
    Available Since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226625-plasmid-map-sequence-id-449160

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  5. pCKC764

    Plasmid
    #226625
    Purpose
    piggyBAC pCAG-hCas9-T2A-Unbiased3
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT S. pyogenes, Chicken, Human)
    EF1a promoter, blasticidin resistance
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV and EF1a
    Available Since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226625-plasmid-map-sequence-id-449160

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  6. pCKC776

    Plasmid
    #226627
    Purpose
    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, mCherry
    Use
    Synthetic Biology
    Tags
    oxygen dependent degron (ODD)
    Expression
    Mammalian
    Promoter
    4xHRE_YB TATA and EF1a
    Available Since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226627-plasmid-map-sequence-id-449157

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  7. pCKC776

    Plasmid
    #226627
    Purpose
    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT S. pyogenes, Human)
    EF1a promoter, mCherry
    Use
    Synthetic Biology
    Tags
    oxygen dependent degron (ODD)
    Expression
    Mammalian
    Promoter
    4xHRE_YB TATA and EF1a
    Available Since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226627-plasmid-map-sequence-id-449157

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  10. pETM33_Nsp4

    Plasmid
    #156474
    Purpose
    Bacterial expression of Sars-CoV2 Nsp4 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156474-plasmid-map-sequence-id-307734

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  11. pETM33_Nsp4

    Plasmid
    #156474
    Purpose
    Bacterial expression of Sars-CoV2 Nsp4 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156474-plasmid-map-sequence-id-307734

    Enlarge

  12. pETM33_Nsp16

    Plasmid
    #156482
    Purpose
    Bacterial expression of Sars-CoV2 Nsp16 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp16 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156482-plasmid-map-sequence-id-307736

    Enlarge

  13. pETM33_Nsp16

    Plasmid
    #156482
    Purpose
    Bacterial expression of Sars-CoV2 Nsp16 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp16 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156482-plasmid-map-sequence-id-307736

    Enlarge

  16. pETM33_Nsp9

    Plasmid
    #156478
    Purpose
    Bacterial expression of Sars-CoV2 Nsp9 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156478-plasmid-map-sequence-id-307616

    Enlarge

  17. pETM33_Nsp9

    Plasmid
    #156478
    Purpose
    Bacterial expression of Sars-CoV2 Nsp9 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156478-plasmid-map-sequence-id-307616

    Enlarge

  18. pETM6-4F2-mCherry

    Plasmid
    #73421
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4F2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P4F2 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73421-plasmid-map-sequence-id-135321

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  19. pETM6-4F2-mCherry

    Plasmid
    #73421
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4F2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P4F2 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73421-plasmid-map-sequence-id-135321

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  20. pETM33_Nsp3d_Ub2_PLPro

    Plasmid
    #156472
    Purpose
    Bacterial expression of Sars-CoV2 Nsp3d_Ub2_PLPro protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp3d_Ub2_PLPro (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156472-plasmid-map-sequence-id-307744

    Enlarge

Showing: 3201 - 3220 of 23227 results