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Showing: 8051 - 8100 of 172228 results

  5. pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)

    Plasmid
    #197508
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…
    Promoter
    CMV and T7
    Available since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197508-plasmid-map-sequence-id-387292

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  6. pET28a/Cas9-Cys

    Plasmid
    #53261
    Purpose
    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
    Depositor
    Hyongbum Kim
    Article
    Ramakrishna et al Genome Res. 2014 Apr 2.
    Insert
    Cas9-Cys
    Use
    Tags
    6X His tag, HA epitope, and NLS
    Expression
    Bacterial
    Mutation
    Additional Cysteine is added to the C-terminal re…
    Promoter
    T7 Promoter
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    53261-plasmid-map-sequence-id-261354

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  11. pET28a-LIC

    Plasmid
    #26094
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    26094-plasmid-map-sequence-id-246717

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  19. pLENTI_TetOn_Hu_BIRC5(WT)

    Plasmid
    #136348
    Purpose
    Lentiviral inducible expression (TetOn) of human BIRC5
    Depositor
    Guillermo de Cárcer
    Insert
    BIRC5 (BIRC5 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    136348-plasmid-map-sequence-id-270811

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  23. mCherry-Sequestosome1-N-18

    Plasmid
    #55132
    Purpose
    Localization: Membrane Scaffold, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Sequestosome1 (SQSTM1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  25. pIR-CMV-SCN2A-Variant-1-IRES-mScarlet

    Plasmid
    #162279
    Purpose
    Eukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
    Depositor
    Al George
    Article
    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do
    Insert
    SCN2A Variant 1, stabilized (SCN2A Human)
    Use
    Tags
    IRES-mScarlet
    Expression
    Mammalian
    Mutation
    IVS intron inserted after bp4551.
    Promoter
    CMV
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162279-plasmid-map-sequence-id-321873

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  29. AAV phSyn1(S)-DreO-bGHpA

    Plasmid
    #50363
    Purpose
    Can be used to generate AAV virus that will express DreO recombinase in neurons from the synapsin promoter
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Has Service
    AAV Retrograde and AAV5
    Insert
    DreO recombinase
    Use
    AAV
    Tags
    none
    Expression
    Mutation
    Sequence optimized for expression in mammalian ce…
    Promoter
    hSyn1
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50363-plasmid-map-sequence-id-308988

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  30. pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV Retrograde)

    Viral Prep
    #154867-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ulrik Gether
    Article
    Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03
    Promoter
    Human Synapsin-1
    Tags
    mCherry (Flp-dependent)
    Available since
    Oct. 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  34. pCAG-2dV-Camui (wt)

    Plasmid
    #220366
    Purpose
    Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)
    Depositor
    Ryohei Yasuda
    Article
    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-
    Insert
    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Rat, Synthetic)
    Use
    Tags
    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    220366-plasmid-map-sequence-id-434512

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  41. TFORF3013

    Plasmid
    #144489
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MEF2A (MEF2A Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    144489-plasmid-map-sequence-id-407363

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  46. pAAV-rtTA

    Plasmid
    #175274
    Purpose
    AAV vector mediating neuron-specific expression of rtTA
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    Reverse tetracycline-controlled transactivator (rtTA)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Synapsin
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    175274-plasmid-map-sequence-id-349083

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Showing: 8051 - 8100 of 172228 results