We narrowed to 18,569 results for: MUT

1. pAAV-hSyn-Htt171-66Q-myc-WPRE

   Plasmid

   #107934

   Purpose

   Adeno-Associated viral (AAV) vector plasmid expressing exon1 of mutant HTT (mHTT, 66 polyQ repeats) in neurons

   Depositor

   Johan Jakobsson

   Article

   Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

   Insert

   Exon 1 of mutant HTT, 66 polyQ repeats (HTT Human)
   

   Use

   AAV

   Promoter

   hSynapsin

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti_EF1a_Flag_TP53_R248W+P278A

   Plasmid

   #183115

   Purpose

   Expresses flag-tagged p53 with both R248W and P278A mutations in mammalian cells

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed proline 278 to alanine and arginine 248 t…

   Promoter

   EF1a

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

   Plasmid

   #102422

   Purpose

   Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector

   Depositor

   Kian Peng Koh

   Article

   Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

   Insert

   Tet1 catalytic domain mutant (Tet1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H1620Y and D1622A mutations in Tet1 catalytic dom…

   Promoter

   TetO-CMV

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSIN-hSNCA-NE

   Plasmid

   #102366

   Purpose

   Lentiviral overexpression of synuclein alpha

   Depositor

   Shu Leong Ho

   Article

   Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

   Insert

   synuclein alpha (SNCA Human)
   

   Use

   Lentiviral

   Tags

   NE

   Expression

   Mammalian

   Mutation

   A silent mutation (333bp downstream of ATG) was i…

   Promoter

   EF-1a

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1α1.1-ChrimsonR-GFP

   Plasmid

   #122063

   Purpose

   AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV2

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   EF1a（1.1kb short version)

   Available Since

   March 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLL1 sgRNA(MS2)-MS2-AIDmono

   Plasmid

   #112127

   Purpose

   empty sgRNA cloning vector with MS2-AIDmono

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   AID mono mutant (AICDA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   N- mutated and C- terminal truncated, H130A/R131E…

   Promoter

   hU6,CBh

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Syn-FLEX-fd [ChrimsonR-tdTomato]

   Plasmid

   #84483

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal. tdTomato is a codon diversified version.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   human synapsin promoter

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-Syn-FLPX-rc [ChrimsonR-tdTomato]

   Plasmid

   #128589

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV8

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato (codon diversified version)

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   Syn

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. 8024 GFP_Kras_G12C_2A_puro

   Plasmid

   #64373

   Purpose

   This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2A oncogene along with puro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Insert

   mouse Kras2A (Kras Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Mutation

   changed glycine 12 to cysteine

   Available Since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. LV-AR-KKKAAA

    Plasmid

    #171219

    Purpose

    Lenti-viral expression of human androgen receptor DNA-binding mutant: K630A-K632A-K633A, mutations at the boundary between AR DNA-binding domain and hinge domain.

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR-KKKAAA (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-K630A-K632A-K633A

    Promoter

    EF-1a

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV ArcLightCo (Q239)-T2A-nls-mCherry

    Plasmid

    #85806

    Purpose

    Genetically encoded voltage sensor ArcLight- codon optimized

    Depositor

    Vincent Pieribone

    Article

    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

    Insert

    ArcLightCo-Q239
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    CMV

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. 8027_GFP-KrasG12C_2B_puro

    Plasmid

    #64372

    Purpose

    This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with puro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Insert

    mouse Kras2B (Kras Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Mutation

    changed glycine 12 to cysteine

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-VPR-dSpCas9[StaPL(TI)]

    Plasmid

    #111508

    Purpose

    Expresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].

    Depositor

    Michael Lin

    Article

    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

    Insert

    VPR-dSpCas9[StaPL(TI)]
    

    Expression

    Mammalian

    Mutation

    The HCV NS3 protease carries F43L, Q80K, and S122…

    Promoter

    PGK

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR-53BP1 1-1972 28A

    Plasmid

    #53447

    Purpose

    Recombinational donor/master vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Gateway

    Mutation

    I914T mutation; All 28 SQ/TQ motifs in N-term of …

    Available Since

    Dec. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid

    #128587

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato (codon diversified version)

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    EF1α (1.1 kb short version)

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLL3 sgRNA(MS2)-MS2-AID3f

    Plasmid

    #112129

    Purpose

    empty sgRNA cloning vector with MS2-AID3f

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    AIDmono 3f mutant (AICDA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    N- mutated and C- terminal truncated, F115Y/C116F…

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLL2 sgRNA(MS2)-MS2-AID3c

    Plasmid

    #112128

    Purpose

    empty sgRNA cloning vector with MS2-AID3c

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    AID mono 3c mutant (AICDA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    N- mutated and C- terminal truncated, F115Y/C116F…

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. 8798 GFP-KrasG12C_2B_hygro

    Plasmid

    #64376

    Purpose

    This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with hygro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Insert

    mouse Kras2B (Kras Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Mutation

    changed glycine 12 to cysteine

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pαH-S-GSAS-B.1.1.7

    Plasmid

    #171747

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-B.1.1.7 variant (UK)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-B.1.1.7 variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Available Since

    July 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. PA-DAD-dark

    Plasmid

    #53096

    Purpose

    Expresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformation

    Depositor

    Ronen Zaidel-Bar, Klaus Hahn

    Article

    Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

    Insert

    LOV(dark)-6aa-DAD
    

    Tags

    6x His and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    L47F and C49S in Venus; C450M mutation in LOV2

    Available Since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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