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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,758 results for: LEA

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Showing: 3281 - 3300 of 15758 results

  2. pcDNA-mCherry-4A/4B-CAAX

    Plasmid
    #87699
    Purpose
    Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells
    Depositor
    Robert Campbell
    Publication
    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-4A/4B-CAAX
    Expression
    Mammalian
    Available since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    87699-plasmid-map-sequence-id-177046

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  3. pTP261

    Plasmid
    #104166
    Purpose
    Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling
    Depositor
    Dirk Görlich
    Publication
    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554
    Insert
    Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
    Tags
    14x Histidine tag and NEDD8 from Brachypodium dis…
    Expression
    Bacterial
    Mutation
    1x Cysteine located at bps 493-495 in full sequen…
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    104166-plasmid-map-sequence-id-200075

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  5. VAMP8-mCherry

    Plasmid
    #92424
    Purpose
    Expression of VAMP8 C-terminally conjugated to mCherry.
    Depositor
    Geert van den Bogaart
    Publication
    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VAMP8 (Vamp8 Mouse)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    A36S (Please see Depositor Comments below)
    Promoter
    CMV
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92424-plasmid-map-sequence-id-182516

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  13. cycLuc_TEVS

    Plasmid
    #119207
    Purpose
    Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Roman Jerala
    Publication
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cyclic luciferase with TEVs cleavage site
    Use
    Luciferase
    Tags
    6xHis and AU1 tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    119207-plasmid-map-sequence-id-232048

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  14. CBX7

    Plasmid
    #25241
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX7_04 (CBX7 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  15. pαH-S-GSAS-2P-OMICRON

    Plasmid
    #180593
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180593-plasmid-map-sequence-id-352871

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  16. NR4A1

    Plasmid
    #25477
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NR4A1 (NR4A1 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  17. pEGFP C3-YAP-deltaC

    Plasmid
    #21126
    Depositor
    Marius Sudol
    Publication
    Tsutomu Oka & Marius et al
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Yes-kinase associated protein (YAP1 Human)
    Tags
    EGFP-C3
    Expression
    Bacterial and Mammalian
    Mutation
    5 Carboxy-terminal amino acids -FLTWL are deleted
    Available since
    June 23, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 3281 - 3300 of 15758 results