We narrowed to 26,579 results for: She
-
Plasmid#68848Purposemutated OPTN was inserted into pEGFP-N1 backboneDepositorInsertoptineurin (OPTN Human)
UseTagsEGFPExpressionMammalianMutationE478G, associated with ALSPromoterCMVAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-eA3Amax-BlastR
Plasmid#152997PurposeC-to-T base editorDepositorInserteA3A-nSpCas9-UGI-UGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine
Plasmid#50456PurposeDouble floxed Gs-coupled rM3D-IRES-mCitrine under the control of human synapsin promoterDepositorInsertrM3D-IRES-mCitrine (CHRM3 Human)
UseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
AMPKA2
Plasmid#36194PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertAMPKA2 (PRKAA2 Human)
UseTagsHis tag with thrombin cleavageExpressionBacterialMutationcontains KINASE DOMAIN onlyPromoterAvailable sinceAug. 8, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
p11d-tRPA-32Ala9
Plasmid#102616PurposeExpression of human RPA with an alanine substituted phosphorylation domain (with the following mutations in RPA32 :S8,11-13,23,29,33,39A,T21A).DepositorUseTagsExpressionBacterialMutationwith an alanine substituted phosphorylation domainPromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
epB_UbC_tdMS2cp-tagRFP-Tx2
Plasmid#119908PurposeePiggyBac mammalian expression plasmid for tandem dimer MS2cp-tagRFP-T fusionDepositorInsertHA-tdMS2cp-tagRFP-Tx2-nls
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-mCherry-LacR-PALB2
Plasmid#71112Purposemammalian expession of mCherry-LacR-PALB2DepositorInsertPALB2 (PALB2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMV/TOAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_AID-Dam-only
Plasmid#136065Purposein vitro transcription for mRNADepositorInsertAID-Dam only
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct-miRFP713
Plasmid#136562PurposeF-actin labeling with a near-infrared fluorescent protein miRFP713DepositorInsertLifeAct-miRFP713
UseTagsmiRFP713ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTubulin-miRFP670nano
Plasmid#127429PurposeHuman alpha-tubulin labeling with near-infrared fluorescent protein miRFP670nanoDepositorInsertmiRFP670nano-human alpha-tubulin
UseTagsmiRFP670nanoExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
p35S-GW-3Avi
Plasmid#127918PurposeGateway destination vector for transcient expression of proteins with C-terminal 3_Avi in plant protoplastDepositorTypeEmpty backboneUseTags3xAviTagExpressionPlantMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBirA-P19
Plasmid#127919PurposeTransient expression of biotin ligase BirA and p19 in plant protoplastDepositorInsertBirA
UseTagsExpressionPlantMutationPromoter35SAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pESC-URA::CYP71AV1/CPR
Plasmid#20118DepositorInsertamorphadiene oxidase and p450 reductase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMarch 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Cas9-NRRH
Plasmid#136924PurposeExpresses Cas9-NRRH in mammalian cellsDepositorInsertCas9-NRRH
UseCRISPRTagsExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-hCOL1A1
Plasmid#140112PurposeExpresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 (COL1A1 Human)
UseTagsmCeruleanExpressionMammalianMutationCOL1A1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD474N-EGFP
Plasmid#68847Purposemutated OPTN was inserted into pEGFP-N1 backboneDepositorInsertoptineurin (OPTN Human)
UseTagsEGFPExpressionMammalianMutationD474N (not linked to any diseases)PromoterCMVAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
GWF058
Plasmid#133609PurposeUAS-mCherry/CMV-Gal4DBD-NS3a-P2a-DNCR2-VPRDepositorInsertGal4DBD-NS3a, DNCR2-VPR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pR96L-EGFP
Plasmid#68846Purposemutated OPTN was inserted into pEGFP-N1 backboneDepositorInsertoptineurin (OPTN Human)
UseTagsEGFPExpressionMammalianMutationR96L, associated with ALSPromoterCMVAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet3 CD Mut
Plasmid#72222PurposeExpress flag-tagged mouse Tet3 catalytic domain - catalytic dead mutant.DepositorInsertTen-Eleven Translocation 3
UseTagsflagExpressionMammalianMutationThe plasmid expresses TET3 catalytic domain (cata…PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKMV-VioD
Plasmid#65350Purposesysthesis of vioD gene in the violacein pathwayDepositorInsertvioD
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUBC_hsPUM1-iRFP670
Plasmid#107609PurposeTransient expression of Pumilio1, fused to iRFP670 in mammalian cells, under a hUBC promoterDepositorInserthsPUM1-iRFP670
UseSynthetic BiologyTagsiRFP670ExpressionMammalianMutationPromoterhuman UBCAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
PLKO-mcherry-luc-puro-shSCR
Plasmid#29780DepositorInsertshSCR
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC-mCherry Rab5
Plasmid#115239PurposeTo express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.DepositorInsertRab5a (RAB5A Homo sapiens, Human)
UseAAVTagsmCherryExpressionMammalianMutationPromoterHuman UbCAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKMV-VioB
Plasmid#65348Purposesysthesis of vioB gene in the violacein pathwayDepositorInsertvioB
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b T7pCONS sfGFP
Plasmid#154467PurposepET15b with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
UseTagsHis-thrombinExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP713-tubulin
Plasmid#136561PurposeHuman alpha-tubulin labeling with a near-infrared fluorescent protein miRFP713DepositorInsertmiRFP713-tubulin
UseTagsmiRFP713ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX429 SpCas9 N863A nickase
Plasmid#68706PurposeSpCas9 N863A nickaseDepositorInsertSpCas9 N863A nicking mutant
UseCRISPRTagsExpressionMammalianMutationPromoterCbhAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
GWF297
Plasmid#133612PurposeTET3G-2xMS2(wt+f6), NLS-MCP-ANR-ANR-P2a-BFPDepositorInsertTET3G-2xMS2(wt+f6), MCP-ANR-ANR, BFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP703-myosin
Plasmid#136565PurposeLC-Myosin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)DepositorInsertemiRFP703-myosin
UseTagsemiRFP703ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-LSD1-K661A-Hygro
Plasmid#104414Purposetransient expression of dCas9-dLSD1 fusion proteinDepositorInsertdLSD1 (KDM1A Human)
UseCRISPRTagsExpressionMammalianMutationIRATp970A0364D (Source Bioscience) has a P405H ch…PromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
HTATIP (2OU2)
Plasmid#33338PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHTATIP (KAT5 Human)
UseTagsHexahistidine tag with Thrombin cleavageExpressionBacterialMutationPromoterT7Available sinceJan. 11, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCW-GFP-CtIP-T847E
Plasmid#71111Purposemammalian expession of GFP-CtlP T847E mutationDepositorInsertCtlP
UseLentiviralTagsGFPExpressionMammalianMutationT847EPromoterTRE promoter, Tet ONAvailable sinceDec. 9, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA mouse NHERF-1 (1-355)
Plasmid#32705DepositorInsertsolute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (Nherf1 Mouse)
UseTagsnoneExpressionMammalianMutationnonePromotercmvAvailable sinceFeb. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-eA3AmaxNG-BlastR
Plasmid#152999PurposeC-to-T base editor, NG PAMDepositorInserteA3A-nSpCas9 NG-UGI-UGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-20X-UAS-ADARcd
Plasmid#81173Purposeidentification of cell-specific targets of RNA binding proteinsDepositorInsertUAS-ADARcd
UseTagsExpressionInsectMutationPromoterUASAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-eA3Amax-T31A-NG-BlastR
Plasmid#153000PurposeC-to-T/G/A base editorDepositorInserteA3A-BE4 T31A-nSpCas9-UGI-UGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4
Plasmid#87733PurposeBacterial expression of full-length S. cerevisiae Vps4DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEVExpressionBacterialMutationPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pvimentin-miRFP713
Plasmid#136569PurposeVimentin labeling with near-infrared fluorescent protein miRFP713DepositorInsertvimentin-miRFP713
UseTagsmiRFP713ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPTN217-577-EGFP
Plasmid#68841Purposedeleted OPTN was inserted into pEGFP-N1 backboneDepositorInsertoptineurin (OPTN Human)
UseTagsEGFPExpressionMammalianMutationaa 210-577 onlyPromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only