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pLenti.CMV/TO_PRKAA2

Plasmid

#74447

Purpose

Lentiviral expression vector encoding untagged WT human AMPK alpha 2

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

AMPK alpha 2 (PRKAA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only
EF1a_CRX_P2A_Hygro_Barcode

Plasmid

#120433

Purpose

Barcoded lentiviral vector to express CRX in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

CRX (CRX Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_ETV2_P2A_Hygro_Barcode

Plasmid

#120436

Purpose

Barcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ETV2 (ETV2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_SOX2_P2A_Hygro_Barcode

Plasmid

#120480

Purpose

Barcoded lentiviral vector to express SOX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SOX2 (SOX2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pNCST-AvicFP4

Plasmid

#129507

Purpose

Expresses AvicFP4 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP4

Expression

Bacterial

Mutation

One of two variants of AvicFP4 tested with essent…

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-mCherry-GJA1-20k-N1

Plasmid

#49861

Purpose

Encodes C-terminal fragment of human connexin 43 initiating at M213 with a V5 C-terminal fusion tag

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

GJA1-20k (GJA1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

GJA1-20k corresponds to residues 213 to 382 to Co…

Promoter

CMV

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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EF1a_TFAP2C_P2A_Hygro_Barcode

Plasmid

#120487

Purpose

Barcoded lentiviral vector to express TFAP2C in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

TFAP2C (TFAP2C Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-CAG-F14F15S-Switch

Plasmid

#99733

Purpose

Flp-based FLEX reporter (from RFP to GFP)

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

TagRFP and EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1a-GFP-P2A-KLF4

Plasmid

#130694

Purpose

Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

human KLF4 (KLF4 Human)

Use

Lentiviral

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF7_P2A_Hygro_Barcode

Plasmid

#120493

Purpose

Barcoded lentiviral vector to express KLF7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF7 (KLF7 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF1_P2A_Hygro_Barcode

Plasmid

#120488

Purpose

Barcoded lentiviral vector to express KLF1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF1 (KLF1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_NEUROG3_P2A_Hygro_Barcode

Plasmid

#120468

Purpose

Barcoded lentiviral vector to express NEUROG3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NEUROG3 (NEUROG3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX_BHLHE40-FlagHA_IRES-ZsGreen1

Plasmid

#149714

Purpose

Lentiviral vector expressing human BHLHE40 with FLAG-HA tags

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

BHLHE40 (BHLHE40 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Promoter

EF1α

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgAMPKa1

Plasmid

#138684

Purpose

Expresses a human AMPKa1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgAMPKa1 human (PRKAA1 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/DEST/hCx43-E2HA-stop

Plasmid

#40909

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

Connexin 43 internal HA tag (GJA1 Human)

Expression

Mammalian

Mutation

HA tag inserted in second extracellular loop

Promoter

CMV

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only
EF1a_HAND2_P2A_Hygro_Barcode

Plasmid

#120447

Purpose

Barcoded lentiviral vector to express HAND2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HAND2 (HAND2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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PB-Neo-TetO-Cdk12_Untagged

Plasmid

#127178

Purpose

Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)

Use

Piggybac transposase vector

Tags

None

Expression

Mammalian

Promoter

TetO Promoter

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-GJA1-S373A

Plasmid

#52879

Purpose

Encodes human Connexin 43 resistant to siRNA with Serine to Alanine mutation at 373

Depositor

Robin Shaw

Article

Smyth et al Traffic. 2014 Mar 10. doi: 10.1111/tra.1

Insert

GJA1 (GJA1 Human)

Expression

Mammalian

Mutation

Serine 373 mutated to Alalnine

Promoter

CMV

Available Since

May 12, 2014

Availability

Academic Institutions and Nonprofits only
EF1a_FOXP1_P2A_Hygro_Barcode

Plasmid

#120441

Purpose

Barcoded lentiviral vector to express FOXP1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

FOXP1 (FOXP1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_LHX3_P2A_Hygro_Barcode

Plasmid

#120456

Purpose

Barcoded lentiviral vector to express LHX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

LHX3 (LHX3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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