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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,967 results for: his

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Showing: 3301 - 3320 of 22967 results

  1. RON2-COMP-blac-flag-his

    Plasmid
    #110962
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    rhoptry neck protein 2 (RON2) (PF3D7_1452000 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    110962-plasmid-map-sequence-id-223581

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  3. PF3D7_0620000-COMP-blac-flag-his

    Plasmid
    #110993
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    secreted ookinete protein 25, putative (PF3D7_0620000 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    110993-plasmid-map-sequence-id-224484

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  5. His8:MBP-tev-Gly-eK-Flv

    Plasmid
    #83383
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83383-plasmid-map-sequence-id-173970

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  16. His6-TEV-LplA W37V and C55M

    Plasmid
    #248819
    Purpose
    Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    W37V and C55M
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248819-plasmid-map-sequence-id-495682

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  17. His6-TEV-LplA W37V and P167I

    Plasmid
    #248816
    Purpose
    Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    W37V and P167I
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248816-plasmid-map-sequence-id-495681

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  18. His6-mCherry-Flag-LplAW37V and T57I

    Plasmid
    #248806
    Purpose
    Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-mCherry-Flag
    Expression
    Mammalian
    Mutation
    W37V and T57I
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248806-plasmid-map-sequence-id-495680

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  19. His6-TEV-LplA W37V and A48R

    Plasmid
    #248821
    Purpose
    Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    W37V and A48R
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248821-plasmid-map-sequence-id-495679

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  20. His6-mCherry-Flag-LplAW37V and P167I

    Plasmid
    #248804
    Purpose
    Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-mCherry-Flag
    Expression
    Mammalian
    Mutation
    W37V and P167I
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248804-plasmid-map-sequence-id-495677

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Showing: 3301 - 3320 of 22967 results