We narrowed to 22,588 results for: his

Showing: 3301 - 3320 of 22588 results

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pBabe-puro/HA-FLAG-RBP2 H483A

Plasmid

#14803

Depositor

William Kaelin

Article

Klose et al Cell. 2007 Mar 9. 128(5):889-900.

Insert

Retinoblastoma Binding Protein 2 (KDM5A Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

changed His 483 to Ala

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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P74-26 Tail Tube Protein (gp93) in pSMT3

Plasmid

#191261

Purpose

Expresses P74-26 gp93 with a His-SUMO tag in bacterial cells

Depositor

Brian Kelch

Article

Agnello et al J Biol Chem. 2023 Feb 13:103021. doi: 10

Insert

P74-26 gp93

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAW-SpyCsn2

Plasmid

#113251

Purpose

E. coli expression vector for S. pyogenes Csn2-MBP-His

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Csn2

Tags

MBP-6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAW-SpyCas1

Plasmid

#113248

Purpose

E. coli expression vector for His-MBP-S. pyogenes Cas1

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Cas1

Tags

6xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAW-SpyCas2

Plasmid

#113249

Purpose

E. coli expression vector for His-MBP-S. pyogenes Cas2

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Cas2

Tags

6xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pYEXC3H

Plasmid

#49026

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pYEXC3GH

Plasmid

#49027

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pMGAHisTev5B

Plasmid

#37221

Depositor

Jon Lorsch

Article

Acker et al Methods Enzymol. 2007;430:111-45.

Insert

eIF5B

Tags

His

Mutation

N-term truncation (contains aa 396-1002)

Available Since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only
GFP scFv [N86/38]

Plasmid

#182095

Purpose

Mammalian Expression of GFP scFv. Derived from hybridoma N86/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting GFP

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-uvsX-H6

Plasmid

#163913

Purpose

Expresses uvsX for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsX

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-nuclear-EKAR4

Plasmid

#174439

Purpose

Nuclear-targeted EKAR4.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

nuclear-EKAR4

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-jGCaMP8s-p10

Plasmid

#162386

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8s

Tags

6xHis

Expression

Insect

Mutation

S26M F286Y Q315H

Promoter

hsp70

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAA3.stelN

Plasmid

#112011

Purpose

Expresses TelN enzyme

Depositor

Stephen Harrison

Article

Jenni et al Science. 2018 May 4;360(6388):552-558. d

Insert

TelN

Tags

strep and his

Expression

Bacterial

Promoter

T7

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEx-NLyn-Venus-Zdk1

Plasmid

#81042

Purpose

mammalian expression of plasma-membrane anchored Zdk1

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Tags

6x His tag, FLAG, NLyn, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-TRBP

Plasmid

#50351

Purpose

bacterial expression of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

Insert

TRBP (TARBP2 Human)

Tags

His and Myc

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiEKAR2G2

Plasmid

#40178

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Use

Lentiviral

Tags

His-Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pEF-Bos MAL Flag

Plasmid

#41554

Depositor

Kate Fitzgerald, Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

MAL (TIRAP Human)

Tags

6xHis and Flag

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 26, 2013

Availability

Academic Institutions and Nonprofits only
pQTEV-ASF1A

Plasmid

#31591

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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p15TV-L

Plasmid

#26093

Purpose

SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Tags

6xHis and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Sept. 6, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST14-SpyCatcher EQ

Plasmid

#35045

Depositor

Mark Howarth

Article

Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

Insert

SpyCatcher EQ

Tags

His6

Expression

Bacterial

Mutation

Deletion of C-terminal till I113, and point mutat…

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only