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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,352 results for: Cas9

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Showing: 3321 - 3340 of 14352 results

  1. pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)

    Plasmid
    #47869
    Purpose
    All-in-one plasmid targeting tdTomato
    Depositor
    Erik Sontheimer, James Thomson
    Publication
    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    NmCas9
    U6pr-tracrRNA
    tdTomato crRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Available since
    Sept. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47869-plasmid-map-sequence-id-68867

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  2. U6_sgRNA_CAG_hSt1Cas9_MTH17CL396

    Plasmid
    #136656
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9:MTH17CL396 Chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and hU6
    Available since
    March 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    136656-plasmid-map-sequence-id-275491

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  3. U6_sgRNA_CAG_hSt1Cas9_TH1477

    Plasmid
    #136655
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9:TH1477 Chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and hU6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136655-plasmid-map-sequence-id-273341

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  4. U6_sgRNA_CAG_hSt1Cas9_LMG18311

    Plasmid
    #136653
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:LMG18311 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and hU6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136653-plasmid-map-sequence-id-273329

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  6. pEJS2697-CMV-Nme2SmuCas9-E932R

    Plasmid
    #228718
    Purpose
    Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nme2SmuCas9-E932R nuclease
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, E932R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228718-plasmid-map-sequence-id-453994

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  9. BPK3258 - human expression plasmid for eSpCas9(1.1)

    Plasmid
    #101176
    Purpose
    Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K848A/K1003A/R1060A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101176-plasmid-map-sequence-id-193999

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  11. PX458-3xHA-SpCas9

    Plasmid
    #130968
    Purpose
    3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Publication
    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xHA-NLS-SpCas9
    Use
    CRISPR
    Tags
    3x HA, EGFP, and NLS
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    Nov. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    130968-plasmid-map-sequence-id-262662

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  13. pUC19-Q8DTE3

    Plasmid
    #103129
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    Q8DTE3(Cas9 coding gene from Treponema denticola (strain ATCC 35405 / CIP 103919 / DSM 14222))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103129-plasmid-map-sequence-id-201893

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  14. pUC19-A7HP89

    Plasmid
    #103082
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    A7HP89(Cas9 coding gene from Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103082-plasmid-map-sequence-id-201722

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  15. AID-C12*-dCas9-BE4max

    Plasmid
    #216731
    Purpose
    Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and dCas9 (Cas9 with D10A and H840A mutations) in mammalian cells.
    Depositor
    Rahul Kohli
    Publication
    Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AID-C12*-dCas9-BE4max (AICDA Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    216731-plasmid-map-sequence-id-429722

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  18. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

    Plasmid
    #199252
    Purpose
    Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    High specificity eSpCas9(1.1)-D10A nickase
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A K848A K1003A R1060A
    Promoter
    CAG promoter
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199252-plasmid-map-sequence-id-391383

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  20. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60230
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Promoter
    U6 and hSyn
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    60230-plasmid-map-sequence-id-490567

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Showing: 3321 - 3340 of 14352 results