We narrowed to 23,310 results for: promoter

1. pSHDY-PL03-mVenus_tetR

   Plasmid

   #137663

   Purpose

   Conjugative vector derived from pSHDY. Constitutive expression of the repressor tetR, as well as aTc-inducible expression of mVenus.

   Depositor

   Ilka Axmann

   Article

   Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

   Insert

   PL03:mVenus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PL03

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2-U6(gCD44v2)-EF1a-BFP-Puro-Cas9(FZ)

   Plasmid

   #117134

   Purpose

   All-in-one expression vector for Cas9 and gRNA against CD44

   Depositor

   Kosuke Yusa

   Insert

   gCD44 v2 (Cd44 Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Promoter

   human U6 promoter, human EF1a promoter

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVPRT-tTR-KRAB

   Plasmid

   #11648

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   hPrion, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCRISPRv2-Stuffer-HepmCherry

   Plasmid

   #192825

   Purpose

   Contains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoter

   Depositor

   Kristin Knouse

   Article

   Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

   Insert

   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-HIRA-polyA

   Plasmid

   #59781

   Purpose

   Express H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   HIRA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. UbC-TRE-mSOD2-tTS-IRES-EGFP

   Plasmid

   #12390

   Depositor

   Zuoshang Xu

   Article

   Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

   Insert

   mSOD2 shRNA (Sod2 Mouse)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-RapGAP

   Plasmid

   #118324

   Purpose

   Expression of RapGAP

   Depositor

   Philip Stork

   Article

   Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

   Insert

   RapGAP
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3E tdTomato

   Plasmid

   #135210

   Purpose

   Red fluorescent tag

   Depositor

   David Tobin

   Article

   Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

   Insert

   tdTomato
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pstb-LAFR5

   Plasmid

   #86169

   Purpose

   pLAFR5 with Smb21651 promoter. pLAFR5 is a broad-host range cosmid vector with double cos sites.

   Depositor

   Kay Jones

   Article

   Jones et al J Bacteriol. 2012 Aug;194(16):4322-31. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   SMb21651 promoter

   Available since

   Feb. 6, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA-EGFP-ELYS-polyA

    Plasmid

    #59746

    Purpose

    Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    EGFP-ELYS
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-emerin-EGFP-polyA

    Plasmid

    #59747

    Purpose

    Express emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    emerin-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDA010.EL222

    Plasmid

    #206801

    Purpose

    J23106.EL222

    Depositor

    James Carothers

    Article

    Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

    Insert

    EL222
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    J23106

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3E p2a-tomato

    Plasmid

    #135211

    Purpose

    Red fluorescent tag

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Insert

    tdTomato
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1C-YFP-shSCR

    Plasmid

    #139647

    Purpose

    Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

    Depositor

    David Cobrinik

    Article

    Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

    Insert

    shSCR
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor comments

    Promoter

    CMV

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-FAK W266A

    Plasmid

    #186149

    Purpose

    For expressing FAK in mammalian cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST_6xUAS:miRFP670-cetn4

    Plasmid

    #192357

    Purpose

    zfCETN4 tagged with miRFP670, expressed under the UAS promoter

    Depositor

    Darren Gilmour

    Article

    Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

    Insert

    CETN4 (cetn4 Zebrafish)
    

    Use

    Tags

    miRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

    Plasmid

    #174137

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAG.mCherry-eDHFR

    Plasmid

    #176201

    Purpose

    Negative control of targeted optogenetic manipulation using TMP-Halo dimerization system

    Depositor

    Michael Lampson

    Article

    Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

    Insert

    mCherry-eDHFR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBEVY-GT

    Plasmid

    #51231

    Purpose

    bi-directional expression vector for yeast, galactose inducible, TRP1 selection

    Depositor

    Charles Miller

    Article

    Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1,10

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPIDNB

    Plasmid

    #169901

    Purpose

    Empty dox inducible vector with piggyBac, insulators, and a DTS

    Depositor

    Richard Schneider

    Article

    Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

    Type

    Empty backbone

    Use

    Aivian expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dox inducible

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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