We narrowed to 4,746 results for: NAP

1. pAAV-hSyn-GFP-IRES-hSNCA (A11P/V70P)

   Plasmid

   #185717

   Purpose

   AAV expression of GFP and human α-Synuclein with A11P and V70P mutations from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (SNCA Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Changed Ala 11 to Pro, Val 70 to Pro

   Promoter

   hSyn

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2

   Plasmid

   #165083

   Purpose

   gRNA 2 of pair B for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1

   Plasmid

   #165085

   Purpose

   gRNA 1 of pair C for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

   Plasmid

   #165086

   Purpose

   gRNA 2 of pair C for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. QuasAr-P2A-hKvb1

   Plasmid

   #154083

   Purpose

   Independent expression of QuasAr and human Kvbeta 1 in pFCK vector

   Depositor

   Michael Hoppa

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

   Insert

   QuasAr-P2A-human Kvb1 (KCNAB1 Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   myc-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. CBSH3+ 3m shRNA-1

   Plasmid

   #160067

   Purpose

   3-point mutation negative control for CBSH3+ shRNA-1 targeting collybistin isoforms with SH3+ domain (plasmid 160066)

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   GGcTGaTTaCAACAAGGATTG (mutations shown in lower c…

   Promoter

   mouse U6 promoter

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. CBSH3- 3m shRNA3

   Plasmid

   #160213

   Purpose

   Knock Down MYDSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA3 targeting collybistin MYD-CBSH3- isoforms (Plasmid #160212)

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   TGTATGACCTCaGGcTGtACCA (mutations shown in lower …

   Promoter

   mouse U6 promoter

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. CBSH3- 3m shRNA2

   Plasmid

   #160211

   Purpose

   Knock Down MTLSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Plasmid #160210)

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   TGACGTTGCTCaGGcTGtACCA (mutations shown in lower …

   Promoter

   mouse U6 promoter

   Available Since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. CBSH3- 3m shRNA1

   Plasmid

   #160209

   Purpose

   Knock Down MQWSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Plasmid # 160208).

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   GATCGGGAATGCTCaGGcTGtACC (mutations shown in lowe…

   Promoter

   mouse U6 promoter

   Available Since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET15-His-∆NGlpG(C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G)

    Plasmid

    #155134

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G variant (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C104A/D243G/L244G/F245G/M247C/S248G/M249G/A250G, …

    Promoter

    T7

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Flag-NSF/T646A

    Plasmid

    #74937

    Purpose

    Mammalian expression of human NSF mutant T646A (mutation in the D2 domain of the protein)

    Depositor

    Elisa Greggio

    Article

    Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

    Insert

    NSF (NSF Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T646A (mutation in the D2 domain of the protein)

    Promoter

    CMV

    Available Since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-Flag-NSF/T645A

    Plasmid

    #74936

    Purpose

    Mammalian expression of human NSF mutant T645A (mutation in the D2 domain of the protein)

    Depositor

    Elisa Greggio

    Article

    Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

    Insert

    NSF (NSF Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T645A (mutation in the D2 domain of the protein)

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCAG-lox-inv[Talpha1-iCre-pA]-lox-2xHA-128-425-Akap9

    Plasmid

    #196897

    Purpose

    Neuron-specific expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to 2xHA-tags.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    2xHA-Akap9(128-425) (Akap9 Rat)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pINTO-N3::hTET3

    Plasmid

    #136364

    Purpose

    Expresses epitope-tagged human TET3 WT in mammalian cells

    Depositor

    Roberto Bonasio

    Article

    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

    Insert

    TET3 (TET3 Human)
    

    Use

    Tags

    2xStrep-tagII, FLAG, and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5 CMV 3xFG eIF4G1

    Plasmid

    #158775

    Purpose

    pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJL1-sfGFP

    Plasmid

    #102634

    Purpose

    In vitro expression of sfGFP from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

    Insert

    sfGFP
    

    Use

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5-EGFP-Tau

    Plasmid

    #46904

    Purpose

    expresses EGFP tagged Tau in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    This WT htau construct contains human four-repeat…

    Promoter

    CMV

    Available Since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDH-EF1a-eFFly-mCherry

    Plasmid

    #104833

    Purpose

    3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detection

    Depositor

    Irmela Jeremias

    Article

    Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

    Inserts

    eFFly
    mCherry
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Stx5S-mCherry

    Plasmid

    #165087

    Purpose

    Expresses Stx5S-mCherry in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    Stx5 (Short isoform) (STX5 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Lacks amino acids 1-54, enocdes short isoform of …

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Bet1-mCherry

    Plasmid

    #209848

    Purpose

    Expresses Bet1-mCherry in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    Bet1 (BET1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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