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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,310 results for: eng

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Showing: 3361 - 3380 of 14310 results

  3. pcDNA3-sACE2v2-Fc(IgG1)

    Plasmid
    #145165
    Purpose
    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Fc region of human IgG1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    145165-plasmid-map-sequence-id-292594

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  4. pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)

    Plasmid
    #114069
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    114069-plasmid-map-sequence-id-220520

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  5. pET-TRSter

    Plasmid
    #78865
    Purpose
    Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element
    Depositor
    Elias Arnér
    Publication
    Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.
    Insert
    Thioredoxin Reductase (Txnrd1 Rat)
    Tags
    Mutated E. coli-type SECIS element (3’ end of ins…
    Expression
    Bacterial
    Promoter
    T7lac
    Available since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    78865-plasmid-map-sequence-id-147493

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  7. pMOD_B2103b

    Plasmid
    #91062
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette (no promoter) for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Promoter
    CmYLCV
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91062-plasmid-map-sequence-id-181865

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  10. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)

    Plasmid
    #114084
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114084-plasmid-map-sequence-id-230536

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  16. pMAL-c2X_MBP-CLmN-H6

    Plasmid
    #234487
    Purpose
    Maltose binding protein N-terminally fused to His-tagged CLmN split intein
    Depositor
    Henning Mootz
    Publication
    Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:
    Insert
    N-terminal Fragment of the CLm Split Intein
    Tags
    Hexahistidine Tag and Maltose Binding Protein (MB…
    Expression
    Bacterial
    Mutation
    T69K, F75H, M118N within the N-terminal fragment …
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    234487-plasmid-map-sequence-id-471039

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  17. pSpdCas9-huTET1CD-T2A-mCherry(PX458)

    Plasmid
    #129027
    Purpose
    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    129027-plasmid-map-sequence-id-256129

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  20. M13-kmR

    Plasmid
    #206861
    Purpose
    M13KE from NEB modified with kanamycin resistance gene and NotI restriction site on g3p ( M13KE-NotI-Kan)
    Depositor
    Irene Chen
    Publication
    Lin et al PLoS One. 2011;6(5):e19991. doi: 10.1371
    Insert
    Kanamycin resistance gene
    Expression
    Bacterial
    Promoter
    lac
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    206861-plasmid-map-sequence-id-412778

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Showing: 3361 - 3380 of 14310 results