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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,582 results for: eng

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Showing: 3361 - 3380 of 14582 results

  1. pcDNA3-ICUE4

    Plasmid
    #181846
    Purpose
    Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.
    Depositor
    Jin Zhang
    Publication
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ICUE4 (RAPGEF3 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Glutamine 122 in Epac1 domain mutated to glutamat…
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    181846-plasmid-map-sequence-id-364236

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  2. DENV4 Rluc Reporter GND mutant Replicon

    Plasmid
    #118586
    Purpose
    For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.
    Depositor
    Radhakrishnan Padmanabhan
    Publication
    Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
    Use
    Yeast-e. col shuttle vector prs424
    Mutation
    The conserved GDD in the NS5 polymerase gene muta…
    Available since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    118586-plasmid-map-sequence-id-227283

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  6. pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

    Plasmid
    #175526
    Purpose
    Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells
    Depositor
    Kai Johnsson
    Publication
    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lifeact-HaloTag11 and Tomm20-HaloTag7
    Expression
    Mammalian
    Mutation
    HaloTag11 = HaloTag7-M175W
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    175526-plasmid-map-sequence-id-343756

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  8. EF1α-AausFP1-Z1

    Plasmid
    #135614
    Purpose
    AausFP1 - bright green fluorescent protein expression from A. australis under EF1a-HTLV promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Publication
    Yang et al PLoS One. 2026 Jul 23;21(7):e0352468. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AausFP1
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF1a-HTLV
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135614-plasmid-map-sequence-id-269404

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  12. pGNW6

    Plasmid
    #122093
    Purpose
    suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells
    Depositor
    Pablo Ivan Nikel
    Publication
    Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    May 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    122093-plasmid-map-sequence-id-243567

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  13. pGNW4

    Plasmid
    #122088
    Purpose
    suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells
    Depositor
    Pablo Ivan Nikel
    Publication
    Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    May 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    122088-plasmid-map-sequence-id-243562

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  16. lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

    Plasmid
    #159086
    Purpose
    Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.
    Depositor
    Jeremy Day
    Publication
    Carullo et al Nucleic Acids Res. 2020 Aug 18. pii: 589
    Insert
    empty crRNA-acRNA backbone, dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    EGFP and FLAG
    Expression
    Mammalian
    Promoter
    hSYN, U6
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    159086-plasmid-map-sequence-id-310691

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Showing: 3361 - 3380 of 14582 results