We narrowed to 5,886 results for: ARL

1. pCAG-memRFP-3xControlgRNA

   Plasmid

   #224567

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   mRFP1
   

   Use

   CRISPR

   Tags

   Membrane localization signal

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-dCas9

   Plasmid

   #47106

   Purpose

   Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Flag and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti_gRNA -hMLHdn-Puro

   Plasmid

   #191105

   Purpose

   Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   hMLH1dn
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   I31 silent mutation (ATA to ATC) to remove early …

   Promoter

   EF-1a

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. LifeAct-14 (LA-14)

   Plasmid

   #158750

   Purpose

   Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.

   Depositor

   Minhajuddin Sirajuddin

   Article

   Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

   Insert

   LifeAct-14
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Deletion mutation for last three amino acids from…

   Promoter

   CMV immediate early

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRE-Tn7-165

   Plasmid

   #118577

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mScarlet
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV hU6-gRNA hUbC-dSaCas9-KRAB-T2A-Thy1.1

   Plasmid

   #194278

   Purpose

   Expresses gRNA and dSaCas9-KRAB from lentiviral vector

   Depositor

   Charles Gersbach

   Article

   McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

   Inserts

   humanized dSaCas9 KRAB T2A Thy1.1
   gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 and hUbC

   Available since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRE-Tn5-165

   Plasmid

   #118545

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mScarlet-I
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRE-Tn7-145

   Plasmid

   #118561

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mScarlet-I
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)

   Plasmid

   #196703

   Purpose

   Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

   Insert

   human RNaseH1 D210N
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D210N, catalytically inactive

   Promoter

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti Lifeact-EGFP BlastR

    Plasmid

    #84383

    Purpose

    Lentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI-Caspase 1

    Plasmid

    #41552

    Depositor

    Kate Fitzgerald

    Article

    Hornung et al Nature. 2009 Mar 26. 458(7237):514-8.

    Insert

    Caspase-1 (CASP1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate-early enhancer/promoter

    Available since

    Dec. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-DIO eNpHR 3.0-EYFP

    Plasmid

    #26966

    Purpose

    AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Article

    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    eNpHR 3.0
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    ER export at 3' end of gene. Trafficking sig…

    Promoter

    EF1alpha

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV500 A-CREB

    Plasmid

    #33371

    Depositor

    Charles Vinson

    Article

    Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

    Insert

    CREB (Creb1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative (see notes)

    Promoter

    CMV

    Available since

    Dec. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti Lifeact-iRFP670 BlastR

    Plasmid

    #84385

    Purpose

    Lentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. TagRFP-T-EEA1

    Plasmid

    #42635

    Depositor

    Silvia Corvera

    Article

    Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Tags

    TagRFP-T

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRE-Tn7-155

    Plasmid

    #118569

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Minitn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRE-Tn5-135

    Plasmid

    #118521

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Minitn5 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOTTC1328 - pAAV EF1a HA-hM3D(Gq)

    Plasmid

    #89149

    Purpose

    An AAV packaging plasmid that expresses HA-tagged hM3D(Gq) excitatory DREADD from the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

    Insert

    hM3D(Gq)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCTRE-CD4

    Plasmid

    #114010

    Purpose

    Dox-inducible Cas9 expression with a CD4 selection marker

    Depositor

    Charles Rudin

    Article

    Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pWMD23

    Plasmid

    #140006

    Purpose

    Expression of ORF1 (Shuffle 1 NLS) and Pong TPase (L418A, L420A)

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Inserts

    ORF1 Shuffle 1 NLS
    Pong TPase LA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    NES sequence mutated L418A, L420A and Ping N-term…

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLVX-RNaseHI-NES-EGFP

    Plasmid

    #196701

    Purpose

    Plasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    bacterial RNaseHI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. MSCV-(mcs) human CSF1 full length-IRES-Puro

    Plasmid

    #86797

    Purpose

    retroviral expression of CSF-1

    Depositor

    Martine Roussel, Charles Sherr

    Article

    Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

    Insert

    CSF-1 (CSF1 Human)
    

    Use

    Retroviral

    Tags

    IRES-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA3-FLAG-SOX4

    Plasmid

    #110360

    Purpose

    Mammalian expression of FLAG-tagged SOX4

    Depositor

    Carlos Moreno

    Article

    Liu et al Cancer Res. 2006 Apr 15;66(8):4011-9. do

    Insert

    SOX4 (SOX4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    lacking 5' UTR

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pOTTC1329 - pAAV EF1a HA-hM4D(Gi)

    Plasmid

    #89150

    Purpose

    An AAV packaging plasmid that expresses HA-tagged hM4D(Gi) inhibitory DREADD from the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

    Insert

    hM4D(Gi)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-CaMKIIa-Flpo

    Plasmid

    #183535

    Purpose

    INTRSECT

    Depositor

    Karl Deisseroth

    Article

    Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

    Insert

    Flpo
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    CaMKIIa

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pTag-BFP-C-h-Rab5a-c-Myc

    Plasmid

    #79801

    Purpose

    Express TagBFP labeled human Rab5a (GTPase located on early endosomal membranes).

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    Rab5a (RAB5A Human)
    

    Use

    Tags

    TagBFP and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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28. pOTTC840 - pAAV 5xATF6 secNLuc

    Plasmid

    #82497

    Purpose

    Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

    Depositor

    Brandon Harvey

    Article

    Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

    Insert

    secNLuc
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5x(ATF6 binding site) c-Fos minimal promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. ePop

    Plasmid

    #50953

    Purpose

    Expresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillations

    Depositor

    Lingchong You

    Article

    Marguet et al PLoS One. 2010 Jul 30;5(7):e11909. doi:

    Inserts

    E lysis protein
    LuxI
    LuxR
    

    Use

    Tags

    Expression

    Mutation

    Early stop codon introduced in mutated luxR resul…

    Promoter

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

    Plasmid

    #162334

    Purpose

    Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    dSaCas9-KRAB
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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