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pCMV-sadCas9-VP64

Plasmid

#115790

Purpose

An AAV vector expressing S. aureus dCas9 fused to VP64.

Depositor

Nadav Ahituv

Article

Matharu et al Science. 2018 Dec 13. pii: science.aau06

Insert

Sa-dCas9

Use

AAV

Tags

NLS and NLS-VP64

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-SEP-GluR1

Plasmid

#24000

Depositor

Robert Malinow

Article

Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Insert

GluR1 (Gria1 Rat)

Tags

GluR1 signal peptide (aa1-22) and SEP (supereclip…

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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PX551

Plasmid

#60957

Purpose

pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.

Depositor

Feng Zhang

Article

Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

Insert

SpCas9

Use

AAV and CRISPR

Tags

HA

Expression

Mammalian

Promoter

pMecp2

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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GWF243

Plasmid

#133608

Purpose

NS3a-CAAX-(IRES)-EGFP-DNCR2-TIAM-P2a-BFP-GNCR1-LARG

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

NS3a-CAAX, EGFP-DNCR2-TIAM, BFP-GNCR1-LARG

Expression

Mammalian

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pTL50_cHS4-CAG-s36GFPmNG-t2A-PuroR-p2A-BFP-polyAcHS4

Plasmid

#223541

Purpose

PUFFFIN cassette with s36GFP-mNeonGreen and tagBFP, strong constitutive promoter CAG, insulated by cHS4 for random integration, puromycin resistance for selection in mammalian cells

Depositor

Sally Lowell

Article

Lebek et al EMBO J. 2024 Jul 12. doi: 10.1038/s44318

Insert

s36GFP-mNeonGreen, tagBFP, PAC

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-ETF1-E55D

Plasmid

#130876

Purpose

Encodes ETF1 with an E55D substitution

Depositor

Prashant Mali

Article

Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

Insert

Eukaryotic transcritional termination factor ETF1-E55D (ETF1 Human)

Expression

Mammalian

Mutation

E55D

Promoter

CAG

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pHL-avitag3

Plasmid

#99847

Purpose

mammalian expression with secretive signal and avi tag for biotinylation

Depositor

Edith Yvonne Jones

Article

Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

Insert

ephrin b2 (EPHB2 Human)

Tags

Avi

Expression

Mammalian

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pST1374-N-NLS-flag-linker-Cas9-H840A

Plasmid

#51129

Purpose

Cas9 nickase optimized for nuclear import.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Insert

Cas9 nickase

Use

CRISPR

Tags

Flag and NLS

Expression

Mammalian

Mutation

H840A

Promoter

CMV

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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peGFP C3-SPG20

Plasmid

#218578

Purpose

Expresses GFP-tagged human SPG20 in mammalian cells

Depositor

Hemmo Meyer

Article

Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

Insert

SPG20 (SPART Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-RILPL1

Plasmid

#244980

Purpose

Express RILPL1 in mammalian cells with mCherry tag

Depositor

Luis Bonet-Ponce

Article

Bonet-Ponce et al J Cell Biol. 2025 Nov 3;224(11):e2024040

Insert

RILPL1 (RILPL1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag-hDcp2

Plasmid

#72213

Purpose

mammalian expression of Flag-tagged human Dcp2

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Q170R (please see depositor's comment below)

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pHL-FcHis

Plasmid

#99846

Purpose

mammalian expression with secretive signal and FC tag

Depositor

Edith Yvonne Jones

Article

Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

Insert

ephrin b2 (EPHB2 Human)

Tags

Fc_His

Expression

Mammalian

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-SynA

Plasmid

#174860

Purpose

Expresses mouse syncytin A in mammalian cells

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

syncytin A (Syna Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-ANXA1-mRuby3

Plasmid

#184888

Purpose

Stable expression of ANXA1-mRuby3 (Annexin A1-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA1 (ANXA1 Human)

Tags

mRuby3

Expression

Mammalian

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4 huZAP(L)

Plasmid

#45907

Depositor

Harmit Malik

Article

Kerns et al PLoS Genet. 2008 Jan;4(1):e21. doi: 10.1

Insert

ZAP(L) isoform (ZC3HAV1 Human)

Tags

HA

Expression

Mammalian

Mutation

M202T, L388F, Q701E, T851I (reference NP_064504.2)

Available Since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-ANXA6-mRuby3

Plasmid

#184892

Purpose

Stable expression of ANXA6-mRuby3 (Annexin A6-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA6 (ANXA6 Human)

Tags

mRuby3

Expression

Mammalian

Mutation

R587K

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6d-V106W

Plasmid

#215827

Purpose

Express CBE6d V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6d V106W-SpCas9-2xUGI (cas9 )

Use

In vitro transcription template

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCXLE-mCherry

Plasmid

#193293

Purpose

episomal vector expressing mCherry fluorescent protein

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-SynB

Plasmid

#174861

Purpose

Expresses mouse syncytin B in mammalian cells

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

syncytin B (Synb Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-S (Wuhan)

Plasmid

#215812

Purpose

Expresses SARS-CoV-2 Spike protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Spike (S )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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