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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,310 results for: LEA

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Showing: 3441 - 3460 of 12310 results

  1. pCMV-bArrestin2-TevC-P2A-TdTomato

    Plasmid
    #89869
    Purpose
    expresses iTango bArrestin2-TevC component and TdTomato bicistronically
    Depositor
    Hyungbae Kwon
    Publication
    Lee et al Nat Methods. 2017 May;14(5):495-503. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bArrestin2-TevC-P2A-TdTomato
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    R173H in TdTomato (please see depositor comments …
    Promoter
    CMV
    Available since
    May 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    89869-plasmid-map-sequence-id-177073

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  3. EBFP2-PCNA-19-SV40NLS-4

    Plasmid
    #55250
    Purpose
    Localization: Nucleus, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    3xSV40 NLS and EBFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55250-plasmid-map-sequence-id-230171

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  8. BB3cN_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104906
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Feb. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    104906-plasmid-map-sequence-id-210667

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  9. MSP2372

    Plasmid
    #73196
    Purpose
    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, and Q926A in SpCas9
    Promoter
    CMV
    Available since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73196-plasmid-map-sequence-id-134659

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  10. pcDNA-NES-ZapCY2

    Plasmid
    #59016
    Purpose
    Cytosolic zinc-binding FRET biosensor
    Depositor
    Amy Palmer
    Publication
    Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
    Tags
    Nuclear Exclusion Signal
    Expression
    Mammalian
    Mutation
    See comment
    Promoter
    CMV
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pKeratin-miRFP670nano

    Plasmid
    #127437
    Purpose
    Keratin labeling with near-infrared fluorescent protein miRFP670nano
    Depositor
    Vladislav Verkhusha
    Publication
    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Keratin-miRFP670nano
    Tags
    miRFP670nano
    Expression
    Mammalian
    Mutation
    Keratin V282L - please see depositor comments bel…
    Promoter
    CMV
    Available since
    July 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127437-plasmid-map-sequence-id-250412

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  18. pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS

    Plasmid
    #107319
    Purpose
    Expresses SpCas9 fused to dSaCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-SaCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 is fused to nuclease dead SaCas9 (D10A, N5…
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107319-plasmid-map-sequence-id-204682

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Showing: 3441 - 3460 of 12310 results