Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 6,571 results for: WUS;

Showing: 3481 - 3500 of 6571 results

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pICSL86977OD:PITG_00582

Plasmid

#173506

Purpose

This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

Depositor

Sophien Kamoun

Article

Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

Insert

PITG_00582_T30-4

Expression

Plant

Promoter

35S

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX305-N-dTAG-CARD8-ZUC

Plasmid

#169990

Purpose

Mammalian expression of the dTAG-CARD8 ZUC (L162M-L537) fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1).

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

dTAG

Expression

Mammalian

Promoter

hPGK

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND F405G + S297A

Plasmid

#169974

Purpose

pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

S297A, F405G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 PAM L368G

Plasmid

#169978

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (L368G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

PAM, L368G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 PAM R394E

Plasmid

#169980

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (R394E) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

PAM, R394E

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 PAM S297A

Plasmid

#169976

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

PAM, S297A

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND E274R + S297A

Plasmid

#169968

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 autoprocessing-deficient FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

S297A, E274R

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND E274R

Plasmid

#169967

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

E274R

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 PAM F370G

Plasmid

#169979

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F370G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

PAM, F370G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND R394E + S297A

Plasmid

#169972

Purpose

pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

S297A, R394E

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 PAM F405G

Plasmid

#169981

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F405G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

PAM, F405G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 PAM E274R

Plasmid

#169977

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

PAM, E274R

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 FIIND (F1079M-L1373)

Plasmid

#166831

Purpose

Gateway pDONR vector containing NLRP1 FIIND (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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dTAG NLRP1 P1214R

Plasmid

#166826

Purpose

dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Expression

Mammalian

Mutation

P1214R

Promoter

hPGK

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-Myosin7B-sgRNA

Plasmid

#154861

Purpose

Lentiviral expression of Cas9 and sgRNA targeting Myosin7B

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

MYO7B sgRNA (MYO7B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ290-D570A-SRDX

Plasmid

#129682

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b-SRDX

Use

CRISPR; Gateway compatible aaccas12b entry clone

Expression

Plant

Mutation

D570A; AacCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ291-D573A-SRDX

Plasmid

#129683

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

BthCas12b-SRDX

Use

CRISPR; Gateway compatible bthcas12b entry clone

Expression

Plant

Mutation

D573A; BthCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aa3.8.3

Plasmid

#136374

Purpose

Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aac.3

Plasmid

#136377

Purpose

Gateway entry clone for AacCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold with MS2 at the third stem loop is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aa1.2.3

Plasmid

#136372

Purpose

Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 with MS2 at the third stem loop is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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