We narrowed to 37,947 results for: eng

1. pTD-CTwinStrep

   Plasmid

   #45939

   Purpose

   use to create C-terminal Twin-Strep-tag fusion proteins

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Tags

   Twin-Strep

   Expression

   Bacterial

   Promoter

   Trc (lactose/IPTG inducible)

   Available Since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPROK AdoHcyase

   Plasmid

   #14683

   Depositor

   Michael Hershfield

   Article

   Coulter-Karis et al Ann Hum Genet. 1989 May . 53(Pt 2):169-7

   Insert

   adenosyl-homocysteine hydrolase (AHCY Human)
   

   Expression

   Bacterial

   Available Since

   May 1, 2007

   Availability

   Academic Institutions and Nonprofits only

3. PGEX-NEDD4

   Plasmid

   #45043

   Depositor

   Shiaw-Yih Lin

   Article

   Yim et al Cancer Cell. 2009 Apr 7;15(4):304-14. do

   Insert

   NEDD4-1 (NEDD4 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   June 6, 2013

   Availability

   Academic Institutions and Nonprofits only

4. AAV-oChIEF-citrine

   Plasmid

   #50974

   Purpose

   AAV transfer vector with mammalian codon-optimized ChIEF-citrine between AAV2 ITR

   Depositor

   Roger Tsien

   Article

   Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

   Insert

   ChIEF-citrine
   

   Use

   AAV

   Tags

   citrine

   Mutation

   ChIEF gene is mammalian codon optimized

   Promoter

   human synapsin promoter

   Available Since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA-myc-HisA-Sirt3

   Plasmid

   #24924

   Depositor

   Toren Finkel

   Article

   Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

   Insert

   Sirt3 (SIRT3 Human)
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pLKO.1-puro U6 sgRNA CAG

   Plasmid

   #50927

   Purpose

   U6 driven sgRNA negative control

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   negative control sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-oChIEF-tdTomato

   Plasmid

   #50977

   Purpose

   AAV transfer vector with mammalian codon-optimized ChIEF-tdTomato between AAV2 ITR

   Depositor

   Roger Tsien

   Article

   Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

   Insert

   ChIEF-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Mutation

   ChIEF gene is mammalian codon optimized

   Promoter

   human synapsin promoter

   Available Since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA6 MCV LTco

   Plasmid

   #40200

   Depositor

   Patrick Moore

   Article

   Shuda et al J Clin Invest. 2011 Sep;121(9):3623-34.

   Insert

   MCPyV LT codon optimized
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

   Plasmid

   #40132

   Purpose

   Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).

   Depositor

   Pawel Pelczar

   Article

   Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

   Type

   Empty backbone

   Tags

   FokI nuclease (ELD)

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

    Plasmid

    #40131

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Tags

    FokI nuclease (KKR)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pS3

    Plasmid

    #50596

    Purpose

    Backbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5

    Depositor

    Jay Keasling

    Article

    Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

    Inserts

    aroE
    aroD
    aroB
    aroG*
    ppsA
    tktA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. human K19

    Plasmid

    #14744

    Depositor

    M Bishr Omary

    Article

    Zhou et al J Biol Chem. 1999 Apr 30. 274(18):12861-

    Insert

    Keratin 19 (KRT19 Human)
    

    Expression

    Mammalian

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCVL SFFV-EF1s HA.NLS.Sce(opt)

    Plasmid

    #31479

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV/Ef1s HA NLS I-SceI
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Mutation

    "opt" indicates the coding sequence has…

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBad-Xis/Int Reset Flipper

    Plasmid

    #38207

    Depositor

    Drew Endy

    Article

    Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

    Inserts

    Bxb1-Excisionase
    Bxb1 Integrase
    

    Use

    Synthetic Biology; Biobrick cloning vector

    Promoter

    pBAD

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBad-Int Set Flipper

    Plasmid

    #38209

    Depositor

    Drew Endy

    Article

    Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

    Insert

    Bxb1 Integrase
    

    Use

    Synthetic Biology; Biobrick cloning vector

    Tags

    His

    Promoter

    pBAD/AraC

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFlag-CMV2-Smyd2

    Plasmid

    #13521

    Depositor

    Shelley Berger

    Article

    Huang et al Nature. 2006 Nov 30. 444(7119):629-32.

    Insert

    Smyd2 (Smyd2 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

17. AAV-flex-oChIEF-citrine

    Plasmid

    #50973

    Purpose

    flexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITR

    Depositor

    Roger Tsien

    Article

    Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

    Insert

    ChIEF-citrine
    

    Use

    AAV

    Tags

    citrine

    Mutation

    inverted and inserted between two lox sites

    Promoter

    human synapsin promoter

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. LRP6-pCS2-VSVG

    Plasmid

    #27282

    Depositor

    Xi He

    Article

    Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Tags

    VSVG

    Available Since

    March 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP-FB-orig BA

    Plasmid

    #13420

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    orig BA
    

    Tags

    Gal11P

    Expression

    Bacterial

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-Gal4

    Plasmid

    #53366

    Purpose

    full-length native Gal4 with stop in Gateway middle entry clone

    Depositor

    Joshua Bonkowsky

    Article

    Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

    Insert

    native (full-length) Gal4
    

    Promoter

    no promoter

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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