We narrowed to 18,510 results for: puro

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pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer

Plasmid

#86195

Purpose

U6 based expression of N meningitidis sgRNA

Depositor

Scot Wolfe

Article

Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

Insert

nmCas9 sgRNA cloning cassete

Use

Lentiviral

Promoter

U6

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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PB-PEmax

Plasmid

#187893

Purpose

All-in-one prime editor piggyBac transposon with PEmax

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

BPSV40 NLS and c-Myc NLS

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Human sgRNA library Gattinara in pRDA_118 backbone

Pooled Library

#136986

Purpose

Designed for assays with limited cell numbers with 2 guides per gene. Compatible with John Doench’s Brunello human genome-wide library.

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only
pscALPS- SIV-MAC251 vpx

Plasmid

#115810

Purpose

Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMAC251 Vpx

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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PresentER-Cassette mCherry

Plasmid

#102943

Purpose

PresentER with removable cassette for cloning of MHC-I antigen (mCherry version)

Depositor

David Scheinberg

Article

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-WT

Plasmid

#91978

Purpose

Expresses FLAG-HA-AGO2 (WT)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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mCRISPRa-v2, genome-wide, top 5 sgRNAs/gene

Pooled Library

#83996

Purpose

Version 2 of the mouse CRISPRa genome wide library

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Use

Lentiviral

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only
mCRISPRi-v2, genome-wide, top 5 sgRNAs/gene

Pooled Library

#83987

Purpose

Version 2 of the mouse CRISPRi genome wide library

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Use

Lentiviral

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only
pLV_Zika_NS2B_Flag

Plasmid

#79637

Purpose

Third generation Self-inactivating lentivector expressing NS2B from Zika virus

Depositor

Vaithi Arumugaswami

Insert

NS2B

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pPL5071_TEF1*-Cre_URA3

Plasmid

#60930

Purpose

Constitutive expression vector for Cre-recombinase containing URA3 selection marker.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Cre

Tags

Driven from a mutant TEF1 promoter with 0.16x act…

Expression

Yeast

Promoter

mutant TEF1

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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LV-TRE-Empty-T2A-dsRedExpress2

Plasmid

#60623

Purpose

Expresses dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

Empty

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pDR-GFPuniv

Plasmid

#46085

Purpose

In vivo recombination assay fluorescent reporter

Depositor

Raymond Monnat

Article

Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

Insert

DR-GFPuniv reporter

Use

Recombination reporter plasmid

Expression

Mammalian

Mutation

E5G and K163R mutations in EGFP relative to wild-…

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pMRXIP GFP-Stx17TM

Plasmid

#45910

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

Syntaxin 17 (STX17 Human)

Use

Retroviral

Tags

GFP

Mutation

deleted amino acids 1-228

Promoter

CMV

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only
MSCV-C 18E7

Plasmid

#37886

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 18 E7 (E7 HPV)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

July 26, 2012

Availability

Academic Institutions and Nonprofits only
pLemiR-NS

Plasmid

#32809

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

non-specific hairpin

Use

Lentiviral

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only

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cyclin D1-HA-pBabe-Hygro

Plasmid

#22703

Depositor

William Kaelin

Article

Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

Insert

cyclin D1 (CCND1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pLKO human shRNA 2 DEPTOR

Plasmid

#21336

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

DEPTOR shRNA 2 (DEPTOR Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pBABE-HA-GOLPH3

Plasmid

#21687

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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mADAM10 E385A in pQCXIP cFLAG

Plasmid

#19137

Depositor

Zena Werb

Article

Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

Insert

ADAM10 (Adam10 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

E385A

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pCRISPaint-HA-PuroR

Plasmid

#80959

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

HA tag

Use

Gene tagging

Promoter

none

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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