Addgene

We narrowed to 29,535 results for: REP

Showing: 3501 - 3520 of 29535 results

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PWPXLD-GFP-Numb-S276D

Plasmid

#133752

Purpose

Numb with mutation at serine 276 converted to aspartic acid cand tagged with GFP cloned into PWPXLD

Depositor

Chay Kuo

Article

Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

Insert

NUMB endocytic adaptor protein (Numb Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

serine 276 converted to aspartic acid

Promoter

EF1-alpha

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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p63RhoGEF580-mVenus-N1

Plasmid

#84338

Purpose

Expresses p63 (580 a.a.) tagged with mVenus

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2016 Nov 11;6:36825. doi: 10.10

Insert

p63RhoGEF (580 aa) (ARHGEF25 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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epiCRISPRe

Plasmid

#135962

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2020

Availability

Academic Institutions and Nonprofits only
pN1-mCherry-NKD2

Plasmid

#176641

Purpose

Expresses NKD2-mCherry fusion protein in mammalian cells

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Use

Tags

mCherry tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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JBEI-6833

Plasmid

#84983

Purpose

Expression of NudB (E. coli) and PMD (S. cerevisiae) are under control of Trc promoter for isopentenol production.

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

NudB-PMD

Use

Tags

Expression

Bacterial

Mutation

Promoter

trc promoter

Available since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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kank2_pJet1.2

Plasmid

#121980

Purpose

for generating kank2 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank2 (LOC571549 Zebrafish)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC myc-mRAP1 I312R

Plasmid

#73137

Purpose

Expression of myc-tagged mRAP1 with single point mutatoin I312R

Depositor

Agnel Sfeir

Article

Yeung et al Cell Rep. 2013 Jun 27;3(6):1847-56. doi:

Insert

Rap1 (Terf2ip Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

isoleucine changed to an arginine at amino acid 3…

Promoter

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-RlACR_477-EYFP

Plasmid

#103771

Purpose

Anion channelrhodopsin from Rhodomonas lens (RHODO) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R1ACR_477

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDOE11 mas:mTq2 transient

Plasmid

#124248

Purpose

Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected plant cells.

Depositor

Attila Fehér

Article

Lajko et al Plant Cell Rep. 2018 Apr;37(4):627-639.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-his-3C_Clu3B6p

Plasmid

#100093

Purpose

expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide

Depositor

Nikolai Sluchanko

Article

Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10

Insert

14-3-3 sigma human (SFN Human)

Use

Tags

HSPB6 peptide and His6

Expression

Bacterial

Mutation

75-77 residues changed to AAA to facilitate cryst…

Promoter

T7

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS_AKAR5’

Plasmid

#114141

Purpose

Mammalian expression of the AKAR5’ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

AKAR5'

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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TOPO pGK

Plasmid

#68452

Purpose

TOPO construct expressing pGK for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Phosphoglycerate Kinase 1 Promoter (PGK1 Human)

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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kank4_pJet1.2

Plasmid

#121982

Purpose

for generating kank4 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank4 (kank4 Zebrafish)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pSN24

Plasmid

#100109

Purpose

expresses a chimeric SOX2-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCitrine-P2A-SOX2 fused to a 'weak' VP16 domain (SOX2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-U6-sgRNA-M9-IRES-CFP

Plasmid

#114731

Purpose

sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

Depositor

Martine Roussel

Article

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

sgRNA M9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RsACR_995-EYFP

Plasmid

#103772

Purpose

Anion channelrhodopsin from Rhodomonas salina (CCMP1319) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin RsACR_995

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GcACR_457-EYFP

Plasmid

#103137

Purpose

Anion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin GcACR_457

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-shMePCE

Plasmid

#113536

Purpose

Retroviral vector designed to knock down MePCE.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shMePCE (MEPCE Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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mVenus-C1-GEFT

Plasmid

#84340

Purpose

Expresses p63 (474 a.a.) tagged with mVenus

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2016 Nov 11;6:36825. doi: 10.10

Insert

GEFT (ARHGEF25 Synthetic, Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-R1ACR_741-EYFP

Plasmid

#103961

Purpose

Highly efficient anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R1ACR_741

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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