We narrowed to 10,972 results for: CAG

1. pNHEJ-RPG

   Plasmid

   #85931

   Purpose

   Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells

   Depositor

   Zhiying Zhang

   Article

   Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-

   Insert

   pPB-CMV-DsRed-polyA-CAG-NHEJ. Puro-T2A-EGFP
   

   Use

   Dual-reporter surrogate

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330_Actb sgRNA / hSpCas9

   Plasmid

   #172832

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCXLE-dCas9VPPH-T2A-GFP-shP53

   Plasmid

   #102897

   Purpose

   EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   dCas9VPPH-T2A-GFP-shP53
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCXLE-dCas9VPP300-T2A-GFP-shP53

   Plasmid

   #102896

   Purpose

   EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   dCas9VPP300-T2A-GFP-shP53
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330_Tuba1b sgRNA / hSpCas9

   Plasmid

   #172835

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAB-HDAC5(mut-H-Y)

   Plasmid

   #114398

   Purpose

   For PYL-HDAC5 H1006Y expression

   Depositor

   Vincent Dion

   Article

   Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

   Insert

   PYL-HDAC5 H1006Y
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   additional D593E compared to NCBI ref NP_005465.2

   Promoter

   CMV

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAB(EXPR-PYL-HDAC5-NEO)

   Plasmid

   #114395

   Purpose

   For PYL-HDAC5 expression

   Depositor

   Vincent Dion

   Article

   Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

   Insert

   PYL-HDAC5
   

   Use

   Tags

   3xFlag-NLS (internal)

   Expression

   Mammalian

   Mutation

   D593E compared with NCBI reference NP_005465.2

   Promoter

   CMV

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAB-HDAC5(mut-H-A)

   Plasmid

   #114397

   Purpose

   For PYL-HDAC5 H1006A expression

   Depositor

   Vincent Dion

   Article

   Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

   Insert

   PYL-HDAC5 H1006A
   

   Use

   Tags

   3xFlag-NLS (internal)

   Expression

   Mammalian

   Mutation

   D593E compared to NCBI ref NP_005465.2

   Promoter

   CMV

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAB-EXPR(PYL-cat_dom_HDAC5)

   Plasmid

   #114401

   Purpose

   For PYL-HDAC5 catalytic domain expression

   Depositor

   Vincent Dion

   Article

   Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

   Insert

   PYL-HDAC5 catalytic domain
   

   Use

   Tags

   3xFlag-NLS (internal)

   Expression

   Mammalian

   Mutation

   S220F and the deletion of M221

   Promoter

   CMV

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAB-EXPR(PYL-Nterm_dom_HDAC5)

    Plasmid

    #114402

    Purpose

    For PYL-HDAC5 N terminal domain expression

    Depositor

    Vincent Dion

    Article

    Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

    Insert

    PYL-HDAC5 N terminal domain
    

    Use

    Tags

    3xFlag-NLS (internal)

    Expression

    Mammalian

    Mutation

    D593E compared to NCBI ref NP_005465.2

    Promoter

    CMV

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. px330_SMAD exon 9 gRNA

    Plasmid

    #68352

    Purpose

    px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.

    Depositor

    Jannik Elverløv-Jakobsen

    Insert

    SMAD exon 9 gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAK_DR30_CasRx_mCherry

    Plasmid

    #134844

    Purpose

    All in one overexpression of CasRx and guideRNA

    Depositor

    Michael Yaffe

    Insert

    RfxCas13d
    

    Use

    CRISPR

    Tags

    T2A mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAK_DR30_CasRx_GFP

    Plasmid

    #134843

    Purpose

    All in one overexpression of CasRx and guideRNA

    Depositor

    Michael Yaffe

    Insert

    RfxCas13d
    

    Use

    CRISPR

    Tags

    T2A EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAK_DR30_CasRx_Puro

    Plasmid

    #134845

    Purpose

    All in one overexpression of CasRx and guideRNA

    Depositor

    Michael Yaffe

    Insert

    RfxCas13d
    

    Use

    CRISPR

    Tags

    T2A Puromycin

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRT029

    Plasmid

    #127969

    Purpose

    double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRT043-CLYBL-DDdcas9-VPH

    Plasmid

    #158091

    Purpose

    Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

    Depositor

    Martin Kampmann

    Article

    Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

    Insert

    CLYBL-CAG-DDdCas9VPH-T2A-GFP
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid

    #201916

    Purpose

    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and Human U6

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330_VCL sgRNA / hSpCas9

    Plasmid

    #172837

    Purpose

    Mammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330_TOMM20 sgRNA / hSpCas9

    Plasmid

    #172836

    Purpose

    Mammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mouse b3 full length

    Plasmid

    #217814

    Purpose

    Full length mouse integrin b3 expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGB3 (Itgb3 Mouse)
    

    Use

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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