We narrowed to 15,991 results for: LEA

1. pc3XB-ZF70

   Plasmid

   #13193

   Depositor

   Daniel Voytas

   Article

   Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

   Insert

   ZF70
   

   Expression

   Mammalian

   Available Since

   Oct. 31, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. Venus-7aa-MAO-pEGFP-C3

   Plasmid

   #166765

   Purpose

   To target Venus to the mitochondrial outer membrane. MAO tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.

   Depositor

   David Andrews

   Insert

   MAO tail anchor (MAOA Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTAL4

   Plasmid

   #31035

   Depositor

   Daniel Voytas, Adam Bogdanove

   Article

   Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

   Insert

   TALEN-FokI nuclease backbone
   

   Tags

   AcV5 and FokI nuclease

   Available Since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXCX-sGFAP-LMO-IRES-tdTomato

   Plasmid

   #178312

   Purpose

   Express the bacterial lactate 2-monooxygenase (LMO) enzyme in astrocytes

   Depositor

   Sergey Kasparov

   Article

   Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

   Insert

   Lactate 2-monooxygenase
   

   Use

   Adenoviral

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   sGFAP

   Available Since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJH807_pJH_LldR_Pcon-sfGFP.2TAG

   Plasmid

   #163738

   Purpose

   Constitutive expression of green fluorescent protein encoding two stop codons

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   lldr gfp.2TAG
   

   Expression

   Bacterial

   Mutation

   gfp.N39TAG,Y135TAG

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJH809_pJH_LldR_Pcon-sfGFP.10TAG

   Plasmid

   #163739

   Purpose

   Constitutive expression of green fluorescent protein encoding ten stop codons

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   lldr gfp.10TAG
   

   Expression

   Bacterial

   Mutation

   gfp with first 10 leucine codons mutated to amber

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSUPER-retro-puro-shNup133-2

   Plasmid

   #87333

   Purpose

   To express shRNA against human Nup133

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   shRNA against human Nup133
   

   Use

   RNAi

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPOR1CB0058

   Plasmid

   #117539

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamiana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   sgRNA (SaCas9)
   

   Expression

   Plant

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSET FLIPE-100u

   Plasmid

   #13539

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E S118W
   

   Tags

   ECFP and Venus

   Expression

   Bacterial

   Mutation

   S118W in binding protein

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLsCas13a-gRNA-1

    Plasmid

    #164865

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-1
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBT374_pattB-pCA-GFP-pA-attBSwa

    Plasmid

    #52553

    Purpose

    for PhiC31 integrase-mediated cassette exchange

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attB (full length)
    GFP
    

    Expression

    Mammalian

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-bSV-1-830

    Plasmid

    #40963

    Depositor

    Elizabeth Luna

    Article

    Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

    Insert

    supervillin (SVIL Bovine)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    amino acids 1-830

    Available Since

    Dec. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

13. knob-s48i48

    Plasmid

    #68391

    Purpose

    Knob protein fused to elastin-like polypeptide (S48I48)

    Depositor

    Andrew MacKay

    Article

    Sun et al J Control Release. 2011 Oct 30;155(2):21

    Insert

    Knob-S48I48
    

    Tags

    Knob

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPS832

    Plasmid

    #1357

    Depositor

    Pamela Silver

    Article

    Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

    Insert

    GSP1 (GSP1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pGEXndoE(E662Q)

    Plasmid

    #47716

    Purpose

    Expresses GST-EndoE(E662Q) from E. faecalis in E. coli

    Depositor

    Vincent Fischetti, Mattias Collin

    Article

    Collin et al J Biol Chem. 2004 May 21;279(21):22558-7

    Insert

    ndoE
    

    Tags

    GST-EndoE(E186Q) and Glutathione S-transferase

    Expression

    Bacterial

    Mutation

    Changed Glu-662 to Gln

    Promoter

    tac

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS423-SgH

    Plasmid

    #163972

    Purpose

    Helper plasmid for cloning gRNAs under the control of the SNR52 promoter.

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pc3XB-ZF5

    Plasmid

    #13104

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    ZF5
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. Hy_pPepck1 eGFP SV40

    Plasmid

    #132644

    Purpose

    Expresses eGFP mRNA from the Drosophila Pepck1 promoter

    Depositor

    Jeremy Wilusz

    Article

    Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

    Insert

    eGFP
    

    Expression

    Insect

    Promoter

    Pepck1

    Available Since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR Bmi1-C

    Plasmid

    #30511

    Depositor

    John Gurdon

    Article

    Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

    Insert

    Bmi1 (Bmi1 Mouse)
    

    Use

    Gateway - donor vector

    Mutation

    NO STOP

    Available Since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAVS1-NEO-CAG-BFP-pA-GFP-pA

    Plasmid

    #149344

    Purpose

    Vector for targeting the Replace reporter to the AAVS1 locus

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    BFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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