We narrowed to 15,788 results for: LEA

1. pTORKm43GW

   Plasmid

   #112897

   Purpose

   Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418).

   Depositor

   Sebastian Schornack

   Article

   Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

   Type

   Empty backbone

   Use

   Oomycete expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28b-MseLig1

   Plasmid

   #107071

   Purpose

   Expresses M. sedula DNA Ligase 1 in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

   Insert

   DNA ligase 1
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFH42

   Plasmid

   #125881

   Purpose

   Level0 Cestrum Yellow Leaf Curling Virus CmYLCV Pol II promoter

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   Level0 Cestrum Yellow Leaf Curling Virus CmYLCV Pol II promoter
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBM13h-sd4U

   Plasmid

   #179690

   Purpose

   Bacterial expression of base editor

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   [sd4U] UGI-TALE3-DddA-G1397-N wildtype
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. PSuperPuroRetro-1660s(hPot1)

   Plasmid

   #11136

   Depositor

   Christopher Counter

   Article

   Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

   Insert

   RNAi against Human Protection of Telomeres 1 (control) (POT1 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pQE30 NFAT5 DBD

   Plasmid

   #13629

   Depositor

   Anjana Rao

   Article

   Lopez-Rodriguez et al Proc Natl Acad Sci U S A. 1999 Jun 22. 9

   Insert

   NFAT5 (NFAT5 Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   DNA binding domain: aa 175 to 471

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pBABEPuro-rnSOX9

   Plasmid

   #63009

   Purpose

   Expresses rat SOX9

   Depositor

   Michael Ruppert

   Article

   Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

   Insert

   SOX9 (Sox9 Rat)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

8. Venus-7aa-Cb5-pEGFP-C1

   Plasmid

   #166764

   Purpose

   To target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.

   Depositor

   David Andrews

   Insert

   Cb5 tail anchor (Cyb5a Rat)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFREE2-eSpCas9-sgRNA

   Plasmid

   #179580

   Purpose

   Expresses eSpCas9 and gRNA that targets ColE1 plasmids for curing

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   eSpCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHJ386

    Plasmid

    #20679

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Moesin (MSN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Lys 253, 254, 262, 263 to Asn; Changed Th…

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pc3XB-ZF70

    Plasmid

    #13193

    Depositor

    Daniel Voytas

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    ZF70
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. Venus-7aa-MAO-pEGFP-C3

    Plasmid

    #166765

    Purpose

    To target Venus to the mitochondrial outer membrane. MAO tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.

    Depositor

    David Andrews

    Insert

    MAO tail anchor (MAOA Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXCX-sGFAP-LMO-IRES-tdTomato

    Plasmid

    #178312

    Purpose

    Express the bacterial lactate 2-monooxygenase (LMO) enzyme in astrocytes

    Depositor

    Sergey Kasparov

    Article

    Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

    Insert

    Lactate 2-monooxygenase
    

    Use

    Adenoviral

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    sGFAP

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJH807_pJH_LldR_Pcon-sfGFP.2TAG

    Plasmid

    #163738

    Purpose

    Constitutive expression of green fluorescent protein encoding two stop codons

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    lldr gfp.2TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    gfp.N39TAG,Y135TAG

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJH809_pJH_LldR_Pcon-sfGFP.10TAG

    Plasmid

    #163739

    Purpose

    Constitutive expression of green fluorescent protein encoding ten stop codons

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    lldr gfp.10TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    gfp with first 10 leucine codons mutated to amber

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLsCas13a-gRNA-1

    Plasmid

    #164865

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSUPER-retro-puro-shNup133-2

    Plasmid

    #87333

    Purpose

    To express shRNA against human Nup133

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    shRNA against human Nup133
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTAL4

    Plasmid

    #31035

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    TALEN-FokI nuclease backbone
    

    Use

    Tags

    AcV5 and FokI nuclease

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEPOR1CB0058

    Plasmid

    #117539

    Purpose

    Level1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamiana

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    sgRNA (SaCas9)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSET FLIPE-100u

    Plasmid

    #13539

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E S118W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    S118W in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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