We narrowed to 12,310 results for: LEA

Showing: 3521 - 3540 of 12310 results

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pL0M-S-mTurquoise2-EC18152

Plasmid

#137074

Purpose

Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

Depositor

Julian Hibberd

Publication

Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

Insert

mTurquoise2 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning

Use

Synthetic Biology

Expression

Bacterial

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pOCC65

Plasmid

#118865

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric EGFP

Depositor

Marc Bickle, PEPC MPI CBG

Publication

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-3C-mEGFP-NotI-ccdB-AscI-stop-HindIII cassette

Tags

HIS6, cleavable with 3C protease, mEGFP

Expression

Insect

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pY008 (pcDNA3.1-hPbCpf1)

Plasmid

#69980

Purpose

Expresses humanized LPbCpf1

Depositor

Feng Zhang

Publication

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pEMS1600

Plasmid

#29274

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PN

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple129 (MKI67 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available since

Nov. 4, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pET28b_Q69L

Plasmid

#186272

Purpose

Expression vector of His-RanQ69L in E.coli

Depositor

Masahito Ikawa

Publication

Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

GTP-binding nuclear protein Ran (Ran Mouse)

Tags

His-tagged at N terminal

Expression

Bacterial

Mutation

Changed Q69 into L

Promoter

T7 promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB N-term-mScarlet

Plasmid

#169218

Purpose

Targeting vector backbone to support a knock-in of mScarlet-Linker at the N-terminus of a target locus

Depositor

Hugo Snippert

Publication

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mScarlet

Use

Targeting vector backbone

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLentiCRISPRv2-NONO 4

Plasmid

#127652

Purpose

Knock-out of human NONO

Depositor

Nicolas Manel

Publication

Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

Insert

NONO sgRNA (NONO Human)

Use

Lentiviral

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pX330 Human 3' HP1a gRNA

Plasmid

#127907

Purpose

WT Cas9 Vector targeting the 3' end of the human HP1a gene

Depositor

Mark Groudine

Publication

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA for Human 3' HP1a

Use

CRISPR

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p3xEGFP-N1

Plasmid

#122163

Purpose

Expression of 3xEGFP

Depositor

Georg Mayr

Publication

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

3xEGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSynapsin_NLS_GFP_P2A_His6_GluA2

Plasmid

#170453

Purpose

Expresses histidine tagged GluA2 in neuronal cells

Depositor

Shigeki Watanabe

Publication

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

GluA2 (Gria2 Mouse)

Use

Lentiviral

Tags

Hexa-histidine

Expression

Mammalian

Promoter

Synapsin

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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RALA

Plasmid

#55657

Purpose

expression of mouse RALA

Depositor

Julian Downward

Publication

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RALA (Rala Mouse)

Tags

GST and Thrombin cleavage site

Expression

Bacterial

Promoter

lac

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pTE3327

Plasmid

#107535

Purpose

Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.

Depositor

Ervin Welker

Publication

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

As crRNA
hAsCpf1(RVR mutant)

Tags

3xHA, NLS, and SV40 NLS

Expression

Mammalian

Mutation

S542R, K548V, N552R

Promoter

CMV and human U6

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pv6_MCPH1_BRCT W706RW815R

Plasmid

#250355

Purpose

Expresses mutant tandem BRCT domain from MCPH1 fused to eGFP. Serves as control.

Depositor

Tuncay Baubec

Publication

da Silva et al Nat Commun. 2025 Nov 20;16(1):10127. doi

Insert

MCPH1 tandem BRCT

Tags

NLS, eGFP and biotintag, TEV cleavage

Expression

Mammalian

Mutation

W706R and W815R

Promoter

CAGGS

Available since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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CRY2olig-PR

Plasmid

#218917

Purpose

Expression of CRY2olig-mCherry fused to a codon-optimised poly-PR sequence

Depositor

Tatyana Shelkovnikova

Publication

Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRY2olig-mCherry tagged poly-PR

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CRY2olig-GR

Plasmid

#218919

Purpose

Expression of CRY2olig-mCherry fused to a codon-optimised poly-GR sequence

Depositor

Tatyana Shelkovnikova

Publication

Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRY2olig-mCherry tagged poly-GR

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hSyn-GRAB_g5-HT3.0mut

Plasmid

#208718

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons

Depositor

Yulong Li

Publication

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut

Use

AAV

Promoter

hSyn

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_g5-HT3.0

Plasmid

#208727

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0 in astrocytes

Depositor

Yulong Li

Publication

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0

Use

AAV

Promoter

GfaABC1D

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp15-StefinA

Plasmid

#182405

Purpose

Bacterial expression of Vesicle Nucleating peptide15-stefinA fusion

Depositor

Dan Mulvihill

Publication

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-stefin (CSTA Human)

Tags

Vesicle Nucleating peptide 15 (VNp15)

Expression

Bacterial

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MacroH2A1.1-c002

Plasmid

#173087

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROH2A1 (MACROH2A1 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

Q162-N369, codon optimized

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_Ado1.0med

Plasmid

#153292

Purpose

Express the medium-affinity adenosine sensor GRAB_Ado1.0med in Cre positive cells

Depositor

Yulong Li

Publication

Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Adenosine sensor GRAB_Ado1.0med (medium affinity)

Use

AAV

Promoter

EFS

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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