We narrowed to 4,748 results for: NAP

1. pAAV.GFAP.iGluSnFR3.v857.PDGFR

   Plasmid

   #178336

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV GFAP iGluSnFR3 v857.PDGFR
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pINTO-N3::hTET3 HxD

   Plasmid

   #136365

   Purpose

   Expresses epitope-tagged human TET3 catalytically dead (HxD mutation) in mammalian cells

   Depositor

   Roberto Bonasio

   Article

   Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

   Insert

   TET3 (TET3 Human)
   

   Use

   Tags

   2xStrepTagII, FLAG, and HA

   Expression

   Mammalian

   Mutation

   HxD (H1077Y, D1079A)

   Promoter

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFB-EPCP1

   Plasmid

   #226783

   Purpose

   Expression of Z-tagged LRRK2 RCKW using Baculoviruses

   Depositor

   Sebastian Mathea

   Article

   Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

   Insert

   Leucine-rich repeat kinase 2 (LRRK2 Human)
   

   Use

   Tags

   His-Z-TEV

   Expression

   Insect

   Mutation

   Amino acids deleted 1-1326

   Promoter

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223 SARS-CoV-2 NSP5

   Plasmid

   #141259

   Purpose

   Gateway-compatible Entry vector, with insert of NSP5 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Has Service

   Cloning Grade DNA

   Insert

   NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available Since

   April 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pBFC0996

   Plasmid

   #186695

   Purpose

   VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

   Depositor

   Jennifer Doudna

   Article

   Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

   Inserts

   tnsA
   tnsB
   tnsC
   tnsD
   tniQ
   cas8-cas5 fusion
   cas7
   cas6
   crRNA
   Tn7 transposon
   2xLguI Cargo Stuffer
   SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLac

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.CAG.iGluSnFR3.v857.SGZ

   Plasmid

   #178334

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV CAG iGluSnFR3 v857.SGZ
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-DIO-PPO-Venus

   Plasmid

   #139505

   Purpose

   AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Has Service

   AAV8 and AAV9

   Insert

   parapinopsin
   

   Use

   AAV

   Tags

   Venus tag following 3x Ala linker

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB-Tet-On-HTR6-RhoA sensor

   Plasmid

   #189614

   Purpose

   Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); HTR6-fused with a sGFP-mScarlet-I based sensor

   Depositor

   David Clapham

   Article

   Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

   Insert

   HTR6 (HTR6 Human)
   

   Use

   Tags

   GFP and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   Tet3G

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223 SARS-CoV-2 NSP6_nostop

   Plasmid

   #149309

   Purpose

   Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pAAV.hSyn.FLEX.iGluSnFR3.v857.IgK-NGR

    Plasmid

    #196226

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGR

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn FLEX iGluSnFR3 v857.NGR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and NGR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRCN0000338462

    Plasmid

    #78158

    Purpose

    shXPO1 targeting CDS

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    XPO1 (XPO1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    hU6 and hU6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pLXI_TRC403 LacZ

    Plasmid

    #111184

    Purpose

    Control inducible vector with LacZ (V5 tagged)

    Depositor

    William Hahn

    Article

    Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

    Insert

    lacZ
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CAG-DIO-PPO-Venus

    Plasmid

    #139504

    Purpose

    AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Has Service

    AAV5

    Insert

    parapinopsin
    

    Use

    AAV

    Tags

    Venus tag following 3x Ala linker

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLXI_TRC401 LacZ

    Plasmid

    #111183

    Purpose

    Control inducible vector with LacZ (V5 tagged)

    Depositor

    William Hahn

    Article

    Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

    Insert

    lacZ
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223 SARS-CoV-2 NSP5_nostop

    Plasmid

    #149308

    Purpose

    Gateway-compatible Entry vector, with insert of NSP5 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pAAV hSyn mCh-IRES-SspB(milli)-BoNT/B(147-441, Y365A)

    Plasmid

    #122985

    Purpose

    AAV plasmid with human synapsin promoter driving mCh and SspB(milli)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT

    Depositor

    Matthew Kennedy

    Article

    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

    Insert

    mCh-IRES-SspB(milli)-BoNT/B(147-441)
    

    Use

    AAV

    Tags

    mCh

    Expression

    Mammalian

    Mutation

    SspB contains A58V,R73Q "milli" mutatio…

    Promoter

    human synapsin

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. DNA-CARzeta-GFP

    Plasmid

    #89344

    Purpose

    2nd generation lentiviral vector which expresses DNA-CAR receptor fused to GFP

    Depositor

    Ron Vale

    Article

    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

    Insert

    DNA-CARzeta (CD247, CD86 Human)
    

    Use

    Lentiviral

    Tags

    SNAPf tag and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR223 SARS-CoV-2 NSP9_nostop

    Plasmid

    #149312

    Purpose

    Gateway-compatible Entry vector, with insert of NSP9 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP9 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. PRKDC gRNA (BRDN0001146589)

    Plasmid

    #77861

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKDC

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKDC (PRKDC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223 SARS-CoV-2 NSP13_nostop

    Plasmid

    #149315

    Purpose

    Gateway-compatible Entry vector, with insert of NSP13 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP13 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge