We narrowed to 22,823 results for: Sis

1. pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS

   Plasmid

   #153443

   Purpose

   E. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc.XDocB
   

   Use

   Tags

   6xHis and ybbr tag

   Expression

   Bacterial

   Mutation

   Serine 199 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Antares-SEPLuc IRESv24

   Plasmid

   #141087

   Purpose

   precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   Antares-T2A-puromycin-IRESv24-SEPLuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-synPCB2.2

   Plasmid

   #139479

   Purpose

   Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

   Insert

   MTS-Fnr-Fd-P2A-MTS-HO1-PcyA-FLAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V554L

   Promoter

   CAG promoter

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNOC-CRISPR-compact

   Plasmid

   #99369

   Purpose

   Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS. No selection marker.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   Cas9-Nlux

   Expression

   Mutation

   Promoter

   Ribi promoter

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMCh-1478

   Plasmid

   #82424

   Purpose

   Plasmid for expression of GST-tagged Nematostella vectensis GW182 in mammalian cells

   Depositor

   Marina Chekulaeva

   Article

   Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

   Insert

   nvGW182
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR-IAA28_no stop

   Plasmid

   #83861

   Purpose

   Gateway donor vector for IAA28 with no stop codon

   Depositor

   Jennifer Nemhauser

   Article

   Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

   Insert

   NP_568478.1 (IAA28 Mustard Weed)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   no stop codon

   Promoter

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTAct2 (GB0210)

   Plasmid

   #68191

   Purpose

   Provides the Arabidopsis thaliana Actin2 gene terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtAct2 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5'UTRAct2 (GB0193)

   Plasmid

   #68178

   Purpose

   Provides the 5'-untranslated region of the Arabidopsis thaliana actin2 gene as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   5'UTR AtAct2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ac 1.8kb red opsin GFP.

   Plasmid

   #72915

   Purpose

   red opsin promoter from Carolina anole lizard (Anolis carolinensis) gDNA (nucleotides21,797 to 187, corresponding to chr2:88663108–88664997 in anoCar2.0) driving GFP

   Depositor

   Joseph Corbo

   Article

   Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

   Insert

   red opsin promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ac 1.8kb red opsin DsRed

    Plasmid

    #72914

    Purpose

    red opsin promoter from Carolina anole lizard (Anolis carolinensis) gDNA (nucleotides21,797 to 187, corresponding to chr2:88663108–88664997 in anoCar2.0) driving DsRed

    Depositor

    Joseph Corbo

    Article

    Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

    Insert

    red opsin promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET23a-MmUBB(SnaBI)

    Plasmid

    #69558

    Purpose

    In vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    UBB (Ubb Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The two last codons (TATTAA; encoding the last ty…

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pET23a-MmUBB(Y305P)

    Plasmid

    #69557

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    UBB (Ubb Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon TAT encoding tyrosine 305 was changed to CC…

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pPAt2S3 (GB0029)

    Plasmid

    #68162

    Purpose

    Provides the Arabidopsis thaliana Seed-specific promoter 2S3 as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    At2S3 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. JW776

    Plasmid

    #64817

    Purpose

    BiFC rCUC2

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    CUC2 (CUC2 Mustard Weed)
    

    Use

    Tags

    N-terminal domain Firefly

    Expression

    Mutation

    rCUC2

    Promoter

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. JW777

    Plasmid

    #64818

    Purpose

    BiFC CUC3

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    CUC3 (CUC3 Mustard Weed)
    

    Use

    Tags

    C-terminal domain Firefly

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIR197

    Plasmid

    #64819

    Purpose

    BiFC rCUC2

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    CUC2 (CUC2 Mustard Weed)
    

    Use

    Tags

    C-terminal domain Firefly

    Expression

    Mutation

    rCUC2

    Promoter

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. MT583

    Plasmid

    #63114

    Purpose

    The gDNA construct is designed for arabidopsis ecotype Est-1 ACD6 expression in other genetic backgrounds.

    Depositor

    Detlef Weigel

    Article

    Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

    Insert

    ACD6 (Accelerated Cell Death 6)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFRT-TODestMYC_RBPMS_KDR

    Plasmid

    #65667

    Purpose

    MYC expression vector with RBPMS encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    introduced HindIII site to exchange FLAG-HA tag w…

    Promoter

    CMV, 2x Tet

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFRT-TODestFLAGHA_RBPMS_KDR_K100E

    Plasmid

    #65093

    Purpose

    Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with K100E mutation for stable cell line generation

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    changed lysine 100 to glutamic acid (KDR backgrou…

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFRT-TODestFLAGHA_RBPMS_KDR_F65A

    Plasmid

    #65092

    Purpose

    Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with F65A mutation for stable cell line generation

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    change phenylalanine 65 to alanine (KDR backbone)

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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