We narrowed to 21,976 results for: Sis

1. 3xFLAG-dCas9/MSCV-EGFP

   Plasmid

   #82613

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g

   Insert

   3xFLAG-dCas9
   

   Use

   Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. NPY-mApple

   Plasmid

   #83498

   Purpose

   Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

   Depositor

   Sebastian Barg

   Article

   Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

   Insert

   NPY (NPY Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-FLEX-SaCas9-U6-sgSlc17a7

   Plasmid

   #124846

   Purpose

   Mutagenesis of Slc17a7

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a7 (Slc17a7 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7C-DNAJB6

   Plasmid

   #177657

   Purpose

   Recombinant protein synthesis of human DNAJB6

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   DNAJB6 (DNAJB6 Human)
   

   Use

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRES-Puro-MGMT

   Plasmid

   #187654

   Purpose

   Expresses hMGMT together with the puromycin resistance gene via an IRES.

   Depositor

   Robert Sobol

   Article

   Li et al Cancers (Basel). 2022 Jul 22;14(15). pii

   Insert

   MGMT (MGMT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRosa26-TG

   Plasmid

   #40026

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   T (ATG)
   beta-globin intron
   Neo
   GFP
   diphteria toxin A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides 1-274 and insertion of FRT, …

   Promoter

   CAG (chicken beta globin promoter and CMV enhance…

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCherry-C1-FAK-HA-V744G

   Plasmid

   #35040

   Depositor

   Anna Huttenlocher

   Article

   Chan et al J Biol Chem. 2010 Apr 9;285(15):11418-26

   Insert

   focal adhesion kinase (Ptk2 Mouse)
   

   Use

   Tags

   2x HA and mCherry

   Expression

   Mammalian

   Mutation

   GFP-FAK-V744G was generated from GFP-FAK using th…

   Promoter

   Available since

   March 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC841 - pAAV minP secNLuc

   Plasmid

   #82498

   Purpose

   Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

   Depositor

   Brandon Harvey

   Article

   Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

   Insert

   secNLuc
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   c-Fos minimal promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. FKBP-alpha(740-977)-GFP

   Plasmid

   #100731

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis (negative control)

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP2A1 (Ap2a1 Mouse)
   

   Use

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   Long isoform

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7C-DNAJB4

    Plasmid

    #177656

    Purpose

    Recombinant protein synthesis of human DNAJB4

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    DNAJB4 (DNAJB4 Human)
    

    Use

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti-SAM-hygro

    Plasmid

    #138955

    Purpose

    Lentiviral vector for co-expressing spCas9, dRNA-MS2, p65 and HSF1 fused to MS2 binding protein, and hygromycin resistance gene

    Depositor

    Nadya Dimitrova

    Article

    Olivero et al Mol Cell. 2020 Feb 20;77(4):761-774.e8.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 AND EF1a

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUB-nHyPer2 (NLS:HyPer2:NLS)

    Plasmid

    #84730

    Purpose

    Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to Nuclei

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    HyPer2 targeted to nucleus
    

    Use

    Tags

    NLS (nuclear localisation signal and NLS (nuclear…

    Expression

    Plant

    Mutation

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Htr5b(3.7)-EGFP-sv40

    Plasmid

    #184411

    Purpose

    AAV expression of EGFP from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.

    Depositor

    Marylka Yoe Uusisaari

    Article

    Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

    Insert

    Mouse Htr5b gene promoter-EGFP (Htr5b Mouse)
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Htr5b gene

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pS848∙CsR

    Plasmid

    #199240

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Gm resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PEM7

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT/Caggs-NRAS G12V-IRES-ASK1

    Plasmid

    #221074

    Purpose

    Expresses NRAS G12V and ASK1 in mammalian cells

    Depositor

    Hidenori Ichijo

    Article

    Odawara et al Commun Biol. 2024 Jun 5;7(1):691. doi: 1

    Inserts

    NRAS (NRAS Human)
    ASK1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUBQ10:AtFLS2

    Plasmid

    #202186

    Purpose

    Express Arabidopsis thaliana FLS2 gene under UBQ10 promoter

    Depositor

    Gitta Coaker

    Article

    Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

    Insert

    Flagellin sensing 2 (FLS2 Mustard Weed)
    

    Use

    Tags

    HA

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUB-nSypHer (NLS:sypHer:NLS)

    Plasmid

    #84731

    Purpose

    Expresses sypHer in plant cells-biosensor for pH targeted to Nuclei

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    nSypHer
    

    Use

    Tags

    NLS (nuclear localisation signal and NLS (nuclear…

    Expression

    Plant

    Mutation

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRE-Tight-FLAG-CYLD-WT

    Plasmid

    #60030

    Purpose

    WT CYLD under control of Tet promoter with N-terminal FLAG tag

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

    Insert

    Cylindromatosis (CYLD Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Nov. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

19. cfmTurquoise-N

    Plasmid

    #139284

    Purpose

    Cys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.

    Depositor

    Ikuo Wada

    Article

    Omae et al Transfusion. 2019 Jul;59(7):2429-2435. d

    Insert

    cfmTurquoise
    

    Use

    Tags

    Multi-cloning sites

    Expression

    Mammalian

    Mutation

    Cys48Ser and Cys70/Met

    Promoter

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGMEH-T38M-750-luxAB-DAS+4

    Plasmid

    #49520

    Purpose

    Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomally

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    T38M-P750-luxAB-DAS+4
    

    Use

    Tags

    DAS+4 (AANDENYSENYADAS)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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