We narrowed to 11,256 results for: NSI

1. pHH0103_DLG4-1/1

   Plasmid

   #91266

   Purpose

   Protein expression and purification of human SH3 domain construct DLG4-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   DLG4-1/1 (DLG4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. YCe2741 HC_Kan_EF1ap_p18

   Plasmid

   #100698

   Purpose

   part designed to occupy position 18 of EMMA. Functional category: Constitutional promoter

   Depositor

   Yizhi Cai

   Article

   Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

   Insert

   EF1a promoter (EEF1A1 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHH0103_CASKIN2-1/1

   Plasmid

   #91261

   Purpose

   Protein expression and purification of human SH3 domain construct CASKIN2-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   CASKIN2-1/1 (CASKIN2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. FN(9-10, RGE)-I27-CC

   Plasmid

   #85396

   Purpose

   MTFM mechanosensor for integrin. Fibronectin domains 9&10 with RGE mutation fused to titan I27 domain with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labeling

   Depositor

   Khalid Salaita

   Article

   Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

   Insert

   Fibronectin (FN) domains 9&10 containing RGE mutation fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   GRGDS motif mutated to GRGES

   Promoter

   T7

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMCh-1539

   Plasmid

   #82432

   Purpose

   Plasmid for expression of GST-tagged human-Nematostella GW182 chimera in mammalian cells

   Depositor

   Marina Chekulaeva

   Article

   Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

   Insert

   hs-nvGW182 chimera
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …

   Promoter

   EF1A

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMCh-1537

   Plasmid

   #82430

   Purpose

   Plasmid for expression of NHA-tagged human-Nematostella GW182 chimera in mammalian cells

   Depositor

   Marina Chekulaeva

   Article

   Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

   Insert

   hs-nvGW182 chimera
   

   Use

   Tags

   NHA

   Expression

   Mammalian

   Mutation

   aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …

   Promoter

   CMV

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)

   Plasmid

   #70195

   Purpose

   Post cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vector

   Depositor

   Richard Murray

   Article

   Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

   Insert

   tetR-deGFP fusion
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMKO.1-puro/hFOXH1 shRNA6

   Plasmid

   #59310

   Purpose

   Knockdown of human FOXH1

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

   Insert

   hFOXH1 shRNA (FOXH1 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

9. Mouse Nanog locus 3' probe

   Plasmid

   #60037

   Purpose

   Cloned genomic fragment of murine Nanog locus used as probe for Southern Blot analysis

   Depositor

   Jacob Hanna

   Article

   Maza et al Nat Biotechnol. 2015 Jun 22. doi: 10.103

   Insert

   Nanog (Nanog Mouse)
   

   Use

   Unspecified; Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

10. TRS6E1b-luc(-732/-721) mutant 3

    Plasmid

    #45386

    Depositor

    Thomas Gelehrter

    Article

    Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

    Insert

    6 copies of hPAI-1 promoter -732/-721 mutated at -723/-721
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    -723/-721 mutated from tgt to aca

    Promoter

    Available since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

11. TRS6E1b-luc(-732/-721) mutant 2

    Plasmid

    #45385

    Depositor

    Thomas Gelehrter

    Article

    Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

    Insert

    6 copies of hPAI-1 promoter -732/-721 mutated at -730/-728
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    mutated at -730/-728 from aca to tgt

    Promoter

    Available since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pBp-FGFR2c-E471Q

    Plasmid

    #45704

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIc (FGFR2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    E471Q

    Promoter

    Available since

    June 19, 2013

    Availability

    Academic Institutions and Nonprofits only

13. BRCA1-(219-408)

    Plasmid

    #35067

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 219-408

    Promoter

    T7

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pLJM1-FIRE-pHLy

    Plasmid

    #170775

    Purpose

    Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

    Depositor

    Aimee Kao

    Article

    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry and mTFP1

    Expression

    Mammalian

    Mutation

    84 nucleotide Signal Sequence switched to the beg…

    Promoter

    CMV

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

    Plasmid

    #128604

    Purpose

    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Inserts

    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pcmv

    Available since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF_PE2

    Plasmid

    #199267

    Purpose

    Mammalian expression of SpCas9 prime editor 2 under EF1α promoter

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    PE2-P2A-mp53DD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ40-903

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiPGK Blast DEST JNKKTRmRuby2

    Plasmid

    #59154

    Purpose

    Lentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    JNK Kinase Translocation Reporter (MAPK8 Human)
    

    Use

    Lentiviral

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mEos3.2FPP

    Plasmid

    #203758

    Purpose

    Encodes the membrane anchor of HRas, including the last 10 residues of the C terminus with 1 farnesylation and 2 palmitoylations, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    HRas c-term (HRAS Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3-EMTB-TurboID-V5

    Plasmid

    #190736

    Purpose

    Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

    Depositor

    Jeremy Baskin

    Article

    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

    Insert

    ensconsin (MAP7 Human)
    

    Use

    Tags

    Myc, TurboID, and V5

    Expression

    Mammalian

    Mutation

    Microtubule binding-domain of ensconsin (amino ac…

    Promoter

    CMV

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiCRISPR v2-dCas9

    Plasmid

    #112233

    Purpose

    Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2

    Depositor

    Thomas Gilmore

    Article

    Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do

    Inserts

    dCas9
    Porumycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed: Aspartic acid 10 to Glycine, Histidine 8…

    Promoter

    EFS-NS

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Orf3a-mCherry

    Plasmid

    #165138

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Orf3a (ORF3a codon optimized SARS-COV-2)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHH0103_HCK-1/1

    Plasmid

    #91273

    Purpose

    Protein expression and purification of human SH3 domain construct HCK-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    HCK-1/1 (HCK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Mito-PyronicSF/pCMV-myc-mito

    Plasmid

    #124813

    Purpose

    Highly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Use

    Tags

    Mitochondrial Sequence Signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHR-FUSN-mCh-Cry2WT

    Plasmid

    #101223

    Purpose

    FUS(1-214) fused to mCh-Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    FUS(aa1-214) (FUS Human)
    

    Use

    Lentiviral

    Tags

    mCherry-Cry2

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. NLS-PyronicSF/pCMV-myc-nuc

    Plasmid

    #124814

    Purpose

    Highly resposive GFP-based pyruvate nanosensor with a nuclear localization signal.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Use

    Tags

    Nuclear Localization Signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Nsp12-EGFP

    Plasmid

    #165116

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp12 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Nsp8-EGFP

    Plasmid

    #165113

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp8 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGL4.23-5xE-box

    Plasmid

    #206841

    Purpose

    The plasmid contains a 312 bp DNA fragment that contained 5 canonical E-boxes (GCCACGTGCA) spaced by 50 nucleotides and cloned into pGL4.23[luc2/minP] (XhoI/HindIII).

    Depositor

    Tassula Proikas-Cezanne

    Article

    Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

    Insert

    5x E-box ((GCCACGTGCA)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Nsp7-mCherry

    Plasmid

    #165134

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp7 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p3HAT1Luc1

    Plasmid

    #236275

    Purpose

    Mammalian expression of human angiotensin II type 1 receptor; N-terminal HA tag, C-terminal Rluc8

    Depositor

    Vsevolod Gurevich

    Article

    Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

    Insert

    angiotensin II type 1 receptor (AGTR1 Human)
    

    Use

    Tags

    3xHA and Rluc8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. MAC-C Sars-CoV-2 NSP12_P314L

    Plasmid

    #194813

    Purpose

    Sars-CoV-2 NSP12_P314L in MAC-C destination vector

    Depositor

    Markku Varjosalo

    Article

    Varjosalo et al 10 May 2024, Research Square

    Insert

    NSP12_P314L (ORF1ab Sars-CoV-2)
    

    Use

    Tags

    MAC-C

    Expression

    Mammalian

    Mutation

    P314L

    Promoter

    Available since

    May 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. mEmerald-Mito-7

    Plasmid

    #54160

    Purpose

    Localization: Mitochondria, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pFUGW-FIRE-pHLy

    Plasmid

    #170774

    Purpose

    Ratiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cells

    Depositor

    Aimee Kao

    Article

    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry and mTFP1

    Expression

    Mammalian

    Mutation

    84 nucleotide Signal Sequence switched to the beg…

    Promoter

    hUbC

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Lentiviral-TOP-dGFP-reporter

    Plasmid

    #14715

    Purpose

    Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).

    Depositor

    Tannishtha Reya

    Article

    Reya et al Nature. 2003 May 22. 423(6938):409-14.

    Insert

    LEF-1/TCF-responsive promoter (Ctnnb1 )
    

    Use

    Lentiviral

    Tags

    d2 eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. UPB-esophagus (UPB5)

    Plasmid

    #180783

    Purpose

    Unique Projection Barcode (UPB5)-Esophagus for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    hM3Dq ( partial ) (CHRM3 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEGFP SNAP23

    Plasmid

    #101914

    Purpose

    Expresses SNAP23 in mammalian cells

    Depositor

    Thierry Galli

    Article

    Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

    Insert

    SNAP 23 (SNAP23 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMSCV-hygro-STING

    Plasmid

    #102598

    Purpose

    Express STING for IFN-beta luciferase reporter assay.

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTRIP-SFFV-mtagBFP-2A STING

    Plasmid

    #102586

    Purpose

    Lentivector to express STING and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLentiCRISPR_zeo_sgTP53

    Plasmid

    #183180

    Purpose

    pLentiCRISPR that is selectable with zeocin with an sgRNA targeting TP53

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgTP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLenti-RIGI-Apex- T2A-EGFP

    Plasmid

    #167288

    Purpose

    Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    APEX2 and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge