We narrowed to 10,207 results for: UTY

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SpCas9-CBE6b-V106W

Plasmid

#215825

Purpose

Express CBE6b V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b V106W-SpCas9-2xUGI (cas9 )

Use

In vitro transcription template

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Bxb1-His

Plasmid

#123132

Purpose

Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Bxb1 integrase

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-scCAK1

Plasmid

#177014

Purpose

Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)

Tags

Polyhistidine-TEV

Expression

Insect

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET28 His6-mEGFP-D4

Plasmid

#226372

Purpose

Production of recombinant mEGFP-D4, a fluorescent probe binding cholesterol

Depositor

Catherine Tomasetto

Article

Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

Insert

D4 (pfoA Clostridium perfringens)

Tags

His6 and monomeric EGFP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-NbALFA

Plasmid

#159987

Purpose

For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

mCherry-NbALFA

Tags

8histidine-HRV3C protease cleavage site

Expression

Bacterial

Mutation

3GS linker between mCherry and NbALFA

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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GST-TACC3-His6

Plasmid

#69106

Purpose

For bacterial expression of recombinant human TACC3 tagged with GST and His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Tags

GST and His6

Expression

Bacterial

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSB819-PKR-hum

Plasmid

#20030

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R (EIF2AK2 Human)

Expression

Yeast

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV9-null

Plasmid

#200182

Purpose

Expresses AAV2 Rep and AAV9 Cap with nonsense mutation at VR8 site

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV9 Cap w/ nonsense mutation at VR8

Use

AAV

Expression

Mammalian

Mutation

Replaced amino acids 586-592 in Cap with nonsense…

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-H2BmTagBFP2-oG

Plasmid

#217976

Purpose

AAV transfer plasmid to express H2B-BFP and codon-optimized Rabies G protein

Depositor

Edward Callaway

Article

Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

Inserts

H2BmTagBFP2
oG

Use

AAV

Tags

mTAGBFP2

Promoter

hSyn

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFW2500

Plasmid

#224213

Purpose

Bacteroides genomic editing tool; IPTG inducible-Fncpf1 and recT

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Insert

Fncpf1 and recT

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pTSin EGFP

Plasmid

#127695

Purpose

Plasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequence

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

EGFP

Use

Synthetic Biology

Available Since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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rAAV2/cMG

Plasmid

#184539

Purpose

AAV packaging plasmid, expressing Rep2 and AAV-cMG capsid

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

Insert

AAV-cMG capsid

Use

AAV; Helper vector for packaging

Mutation

changes on AAV9 capsid: K449R, A589D; insertion o…

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFR-PATagRFP

Plasmid

#31950

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

Insert

PATagRFP

Tags

EGF receptor (EGFR)

Expression

Mammalian

Mutation

R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

Promoter

CMV

Available Since

Oct. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pSD141B

Plasmid

#218139

Purpose

Lentiviral vector expressing evolved degron SD56 fused to eGFP with mCherry control.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD56-eGFP-IRES2-mCherry

Use

Lentiviral

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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Human RNF149-3X FLAG

Plasmid

#225332

Purpose

Lentiviral expression of human RNF149 with 3x FLAG tag in mammalian cells

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF149 (RNF149 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSin Helper

Plasmid

#127694

Purpose

Plasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

Sindbis Glycoproteins

Use

Synthetic Biology

Available Since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits
pEF Synaptophysin-mRuby

Plasmid

#188980

Purpose

Synaptic buton marker

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Synaptophysin (Syp Rat, Synthetic)

Expression

Mammalian

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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iCAP-ROOT

Plasmid

#192262

Purpose

AAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsid

Depositor

Li Ye

Article

Wang et al Nature. 2022 Sep;609(7927):569-574. doi:

Insert

Synthetic construct for ROOT VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET15TV-L-AldA WT

Plasmid

#187976

Purpose

Expresses human aldolase A

Depositor

Audrey Lamb

Article

Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

Insert

AldA WT (ALDOA Human)

Tags

Hexahistag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Human RNF130-3X FLAG

Plasmid

#225331

Purpose

Lentiviral expression of human RNF130 with 3x FLAG tag in mammalian cells

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF130 (RNF130 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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