We narrowed to 6,366 results for: wus

1. pCMV-hA3A-BE3-D131Y

   Plasmid

   #113424

   Purpose

   Expresses hA3A-BE3-D131Y in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3A-BE3-D131Y (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_D131Y

   Promoter

   CMV

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-hSyn-Galpha-13 ONE-GO

   Plasmid

   #204337

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   hSyn Galpha-13 ONE-GO (GNA13 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. MiniCoopR mitfa:NRAS

   Plasmid

   #118847

   Purpose

   Expresses human NRAS Q61R mutant and zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   NRAS Q61R (NRAS Human, Zebrafish)
   

   Use

   Zebrafish expression, tol3

   Tags

   Expression

   Mutation

   NRAS Q61R

   Promoter

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpyTag-RBD

   Plasmid

   #159999

   Purpose

   Expresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY095

   Plasmid

   #84744

   Purpose

   Expresses huLbCpf1-T2A-GFP and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   GFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax

   Plasmid

   #190153

   Purpose

   Expresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

   Depositor

   Brad Zuchero

   Article

   Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

   Insert

   Vamp2 (Vamp2 Mouse)
   

   Use

   AAV

   Tags

   P2AT2A-EGFP-caax

   Expression

   Mammalian

   Mutation

   Truncation that includes only AA #1-94

   Promoter

   MBP

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#2

   Plasmid

   #172984

   Purpose

   CRISPRi for TAOK2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   sgTAOK2i (TAOK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EF1a_HAND2_P2A_Hygro_Barcode

   Plasmid

   #120447

   Purpose

   Barcoded lentiviral vector to express HAND2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   HAND2 (HAND2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lentiCRISPR v2-sgDHODH-4

   Plasmid

   #186022

   Purpose

   knock out DHODH in mammalian cells

   Depositor

   Boyi Gan

   Article

   Mao et al Nature. 2021 May;593(7860):586-590. doi:

   Insert

   Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode

    Plasmid

    #170220

    Purpose

    Barcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CDK1 (CDK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    T14A, Y15F

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. LZF1735 pAAV_hSyn-DiO-somArchon1-EGFP-P2A-somCheRiff

    Plasmid

    #126512

    Purpose

    Optopatch4. Simultaneous optical recording and manipulation of electrical activity in neurons

    Depositor

    Adam Cohen

    Article

    Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

    Insert

    somArchon1-EGFP-P2A-somCheRiff
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-hA3H-BE3

    Plasmid

    #113416

    Purpose

    Expresses hA3H-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3H-BE3 (APOBEC3H Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTMB341_ZFcharm_Prnp_SCR

    Plasmid

    #220844

    Purpose

    AAV genome expressing ZFcharm targeting Prnp; scrambled self-silencing binding site

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    ZFcharm
    

    Use

    AAV

    Tags

    HAtag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. lentiCRISPR v2-sgDHODH-2

    Plasmid

    #186023

    Purpose

    knock out DHODH in mammalian cells

    Depositor

    Boyi Gan

    Article

    Mao et al Nature. 2021 May;593(7860):586-590. doi:

    Insert

    Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124431

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafish

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Use

    Zebrafish (if expression level is too high in zeb…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMIG II SIN-SV40-mCherry-SHP1

    Plasmid

    #180799

    Purpose

    Expression of mouse SHP1 fused to N-terminal mCherry

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    SHP1 (Nr0b2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-alpha-E-Catenin 3xFP4 (895)

    Plasmid

    #67936

    Purpose

    Mammalian expression of alpha E catenin

    Depositor

    Alpha Yap

    Article

    Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

    Insert

    alpha E catenin (Ctnna1 Mouse)
    

    Use

    Tags

    3xFP4 and EGFP

    Expression

    Mammalian

    Mutation

    N-terminal domain

    Promoter

    CMV

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

18. EF1a_KLF4_P2A_Hygro_Barcode

    Plasmid

    #120455

    Purpose

    Barcoded lentiviral vector to express KLF4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBB75-ncRNA

    Plasmid

    #194087

    Purpose

    Expresses Ec86 ncRNA in Escherichia coli

    Depositor

    Tingting Zou

    Article

    Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

    Insert

    Ec86 ncRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NFAST(low)-mApple-6xHp

    Plasmid

    #214412

    Purpose

    Lipid droplet localization of low-affinity NFAST fused to mApple-6xHp

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspA-N
    

    Use

    Tags

    mApple-6xHp

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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