We narrowed to 6,679 results for: Gca

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gRNA-eGFP-Reporter

Plasmid

#60719

Purpose

Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

gRNA (5'-AAAGGTCGAGAAACTGCAAA-3')

Use

CRISPR

Expression

Mammalian

Promoter

U6 and mouse U6

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-hSOX9-1

Plasmid

#40644

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-hSOX9-1 (SOX9 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Actb KI

Plasmid

#131479

Purpose

Endogenous tagging of βactin: N-terminal (amino acid position: D2)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Actb, Actb Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDonor-tBFP-NLS-Neo (Universal)

Plasmid

#80767

Purpose

Universal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Insert

PITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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HyGCaMP6s

Plasmid

#102558

Purpose

Expression of GCaMP6s in Hydra

Depositor

Rafael Yuste

Article

Dupre et al Curr Biol. 2017 Apr 24;27(8):1085-1097.

Insert

GCaMP6s

Use

Hydra expression

Tags

Actin/GCaMP6s fusion protein (C terminal on backb…

Available Since

Oct. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pTorPE-R-GECO1

Plasmid

#32465

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

R-GECO1.0

Use

Lab constructed

Tags

6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…

Expression

Bacterial

Mutation

Substitutions relative to the mApple-derived anal…

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pDonor-tBFP-NLS-Neo (Universal)

Plasmid

#80767

Purpose

Universal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Insert

PITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pORANGE Tubb3-GFP KI

Plasmid

#131497

Purpose

Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Tubb3, Tubb3 Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

Plasmid

#207664

Purpose

Neuropeptide degradation

Depositor

Sung Han

Article

Kim et al bioRxiv. 2023 Jan 20:2023.01.19.524797.

Insert

NEP (MME Human)

Use

AAV and Cre/Lox

Tags

mRUBY3

Mutation

Addition of signaling peptide (POMC) on the N-ter…

Promoter

hSynapsin

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTorPE-R-GECO1

Plasmid

#32465

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

R-GECO1.0

Use

Lab constructed

Tags

6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…

Expression

Bacterial

Mutation

Substitutions relative to the mApple-derived anal…

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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AAV_CWSL.hSyn.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

Plasmid

#107737

Purpose

In the presence of Cre, drives neuron-specific expression of both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.

Depositor

Rylan Larsen

Insert

synaptophysin-GCaMP6s, mRuby3

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hSyn

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_BiSSTe4_GCaMP6f

Plasmid

#213947

Purpose

AAV construct expressing GCaMP6f driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_GCaMP6f

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

GCaMP6f

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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Reckleen_2_SpGCas9

Plasmid

#233457

Purpose

Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 2 RECKLEEN plasmid

Use

CRISPR

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_BiPVe4_GCaMP6f

Plasmid

#213939

Purpose

AAV construct expressing GCaMP6f driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_GCaMP6f

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

GCaMP6f

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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5HT6-GCaMP5G

Plasmid

#48340

Purpose

Fluorescent reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

GCaMP5G

Tags

5HT6

Expression

Mammalian

Available Since

Nov. 26, 2013

Availability

Academic Institutions and Nonprofits only
Phi3-GCaMP6f

Plasmid

#106298

Purpose

CMV driven GFP based calcium (Ca2+) reporter

Depositor

Sanford Simon

Article

Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

Insert

GCaMP6f

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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HyGCaMP6s

Plasmid

#102558

Purpose

Expression of GCaMP6s in Hydra

Depositor

Rafael Yuste

Article

Dupre et al Curr Biol. 2017 Apr 24;27(8):1085-1097.

Insert

GCaMP6s

Use

Hydra expression

Tags

Actin/GCaMP6s fusion protein (C terminal on backb…

Available Since

Oct. 18, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_CWSL.hSyn.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

Plasmid

#107737

Purpose

In the presence of Cre, drives neuron-specific expression of both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.

Depositor

Rylan Larsen

Insert

synaptophysin-GCaMP6s, mRuby3

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hSyn

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-GCaMP7f

Plasmid

#135539

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to GCaMP7f

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Tags

GCaMP7f

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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elavl3:SomaGCaMP7f

Plasmid

#158760

Purpose

Cell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP7

Use

Synthetic Biology

Promoter

HuC promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Cx36-GCaMP

Plasmid

#123604

Purpose

Mouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cells

Depositor

John O'Brien

Article

Moore et al eNeuro. 2020 Mar 11. pii: ENEURO.0445-19

Insert

Connexin 36-GCaMP3 fusion (Gjd2 Mouse)

Tags

GCaMP3

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_BiSSTe10_GCaMP6f

Plasmid

#213818

Purpose

AAV construct expressing GCaMP6f driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_GCaMP6f

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

GCaMP6f

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBJ089_zACNE20_myl7_Voltron1_P2A_jGCaMP7f

Plasmid

#209283

Purpose

Expresses Voltron1 and jGCaMP7f in the zebrafish heart

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

Voltron1_P2A_jGCaMP7f

Use

Zebrafish expression

Promoter

zACNE20_myl7

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-GCaMP3

Plasmid

#32644

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

GCaMP3

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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5HT6-GCaMP5G

Plasmid

#48340

Purpose

Fluorescent reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

GCaMP5G

Tags

5HT6

Expression

Mammalian

Available Since

Nov. 26, 2013

Availability

Academic Institutions and Nonprofits only
pCAG vGlut1 GCaMP5G

Plasmid

#105008

Purpose

Presynaptic calcium sensor

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

SLC17A7

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG_eGCaMP(2+)

Plasmid

#201148

Purpose

expresses eGCaMP2+ in mammalian cells

Depositor

Andre Berndt

Article

Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

Insert

eGCaMP2+

Expression

Mammalian

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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Sa-SlugCas9

Plasmid

#163797

Purpose

Expresses Sa-SlugCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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Phi3-GCaMP6f

Plasmid

#106298

Purpose

CMV driven GFP based calcium (Ca2+) reporter

Depositor

Sanford Simon

Article

Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

Insert

GCaMP6f

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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ins:GCaMP6s; cryaa:RFP

Plasmid

#110285

Purpose

A zebrafish insulin promoter driving the calcium indicator, GCaMP6s. Contains red eye marker, and is flanked with I-SceI sites.

Depositor

Nikolay Ninov

Article

Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

Insert

GCaMP6s

Use

Zebrafish

Promoter

insulin

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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