We narrowed to 4,976 results for: puc

1. pUC18T-mini-Tn7T-Gm

   Plasmid

   #63121

   Purpose

   mini-Tn7 vector

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 17, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pUC19/NLS-mChe

   Plasmid

   #183162

   Purpose

   Construct for transient expression of cell nucleus-targeted mCherry in plants.

   Depositor

   Qinlong Zhu, Yao-Guang Liu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   NLS-mCherry
   

   Use

   Tags

   16 aa nuclear localization signal and 16 aa nucl…

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19/Mito-mChe

   Plasmid

   #183167

   Purpose

   Construct for transient expression of mitochondrion-targeted mCherry in plants.

   Depositor

   Qinlong Zhu, Yao-Guang Liu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   Mito-mCherry
   

   Use

   Tags

   OsCOX11, Oryza sativa cytochrome c oxidase assemb…

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUCM-CLYBL-hNIL

   Plasmid

   #105841

   Purpose

   Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron

   Depositor

   Michael Ward

   Article

   Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   ISL LIM Homeobox 1 (ISL1 Human)
   LIM Homeobox 3 (LHX3 Human)
   mCherry
   rtTA3G
   

   Use

   CRISPR and TALEN

   Tags

   Expression

   Mutation

   Promoter

   CAG, EF-1alpha, and TRE3G

   Available since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET246-pUC57 12xMS2V6

   Plasmid

   #104390

   Purpose

   MS2V6 cloning

   Depositor

   Evelina Tutucci, Robert Singer

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   12xMS2V6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19/PLIN2-GFP

   Plasmid

   #202194

   Purpose

   knock-in donor vector of GFP to the C-terminus of endogenous human PLIN2

   Depositor

   James Olzmann

   Article

   Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

   Insert

   PLIN2 (PLIN2 Human)
   

   Use

   Cloning vector

   Tags

   GFP knock-in

   Expression

   Mutation

   Promoter

   lac

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-gCarvi-T2A

   Plasmid

   #190333

   Purpose

   Fluorescent reporter for cAMP

   Depositor

   Naoto Saitoh

   Article

   Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

   Insert

   gCarvi
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUCmini-iCAP-PHP.B

   Plasmid

   #103002

   Purpose

   non-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

   Insert

   Synthetic construct isolate AAV-PHP.B VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC gRNA Shuttle

   Plasmid

   #47024

   Purpose

   Encodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Insert

   gRNA Shuttle
   

   Use

   CRISPR; Cas9

   Tags

   Expression

   Plant

   Mutation

   G to T cloning mutation at position 323

   Promoter

   Medicago truncatula U6.6

   Available since

   Sept. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-mAirn-R-loop

    Plasmid

    #134900

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    mAirn promoter (Airn Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC18T-mini-Tn7T-Tp

    Plasmid

    #65024

    Purpose

    TpR mini-Tn7 vector, mobilizable

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pUC19-hAPOE-R-loop

    Plasmid

    #134901

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    APOE promoter (APOE Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC19-hSNRPN-R-loop

    Plasmid

    #134902

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    hSNRPN promoter (SNRPN Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC19-Mu-switch-R-loop

    Plasmid

    #134899

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    mouse IgH switch repeat region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET259-pUC57 24xMS2V6

    Plasmid

    #104391

    Purpose

    MS2V6 cloning

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUCmini-iCAP-PHP.eC

    Plasmid

    #201903

    Purpose

    non-standard AAV2 rep-AAV-PHP.eC cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Shay et al Sci Adv. 2023 Apr 21;9(16):eadg6618. doi

    Insert

    Synthetic construct isolate AAV-PHP.eC VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUCmini-iCAP-PHP.V1

    Plasmid

    #127847

    Purpose

    non-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

    Insert

    Synthetic construct isolate AAV-PHP.V1 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC-UAS1B8-Leum

    Plasmid

    #44316

    Depositor

    Hal Alper

    Article

    Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

    Insert

    UAS1B8-Leum promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    UAS1B8-Leum

    Available since

    April 18, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pUC19 Giles integrase

    Plasmid

    #170049

    Purpose

    pUC19 with Giles integrase

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Insert

    GIles Integrase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC-hrDNA-12.0

    Plasmid

    #206320

    Purpose

    A plasmid containing a 12.0 kb EcoRI fragment that corresponds to sequences between 30.5 and 42.5 kb of the human rDNA repeat (GenBank: U13369)

    Depositor

    Brian McStay

    Article

    van Sluis et al Methods Mol Biol. 2016;1455:3-14. doi: 1

    Insert

    Intergenic spacer of human rDNA repeat
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUC18-mini-Tn7T-Gm-GW

    Plasmid

    #64961

    Purpose

    mini-Tn7 Gateway destination vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pUCmini-iCAP-PHP.N

    Plasmid

    #127851

    Purpose

    non-standard AAV2 rep-AAV-PHP.N cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

    Insert

    Synthetic construct isolate AAV-PHP.N VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUC18R6K-mini-Tn7T

    Plasmid

    #64958

    Purpose

    mini-Tn7 base vector with transcriptional terminator

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pUC57-Amp_CRISPIE donor B5

    Plasmid

    #172846

    Purpose

    CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUCmini-iCAP-AAV9.452sub.LUNG1

    Plasmid

    #184592

    Purpose

    non-standard AAV2 rep-AAV9.452sub.LUNG1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system for mouse lung transduction after intravenous injection

    Depositor

    Viviana Gradinaru

    Article

    Goertsen et al Mol Ther Methods Clin Dev. 2022 Aug 1;26

    Insert

    Synthetic construct isolate AAV9.452sub.LUNG1 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    7 amino acid substitution between VP1 452 and VP1…

    Promoter

    p41

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUC57-Amp_CRISPIE donor B2

    Plasmid

    #172843

    Purpose

    CRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    CRISPIE designer exon (phase 0-0) encoding mRuby3
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUC57-HBB-nLuc

    Plasmid

    #175432

    Purpose

    Used to generate IVT RNA for translation extracts

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    HBB-nLuc
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUC57-rm_gCarvi-T2A

    Plasmid

    #190334

    Purpose

    Ratiometric reporter for cAMP

    Depositor

    Naoto Saitoh

    Article

    Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

    Insert

    ratiometric gCarvi
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUC18/promSau/Lysostaphin

    Plasmid

    #125766

    Purpose

    Production of staphylolytic protein lysostaphin from a constitutive promoter

    Depositor

    Benedykt Wladyka

    Article

    Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

    Insert

    lysostaphin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sequence corresponding to the mature form protein

    Promoter

    pemIK constitutive promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. His Puc (aa 1-393)

    Plasmid

    #52295

    Purpose

    Bacterial expression of His tagged Drosophila Puc fragment

    Depositor

    Brenda Schulman

    Article

    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

    Insert

    Puc (puc Fly)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only