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Addgene

We narrowed to 6,889 results for: GFP expression plasmids

Showing: 851 - 900 of 6889 results
  1. pICH-chl-roGFP2-GPXC71S

    Plasmid
    #191703
    Purpose
    Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a plastid-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191699 and 191701
    Depositor
    Insert
    chl-redox(ro)GFP2-GPXC71S
    Use
    Binary agrobacterium ti plasmid
    Tags
    Expression
    Plant
    Mutation
    Peroxidatic Cysteine 71 replaced with Serine
    Promoter
    CaMV 35S
    Available since
    March 10, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

    Plasmid
    #154786
    Purpose
    Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE
    Depositor
    Insert
    Adenosine deaminse (Adar Fly, Synthetic)
    Use
    Lentiviral
    Tags
    GFP, V5, and mCherry
    Expression
    Mammalian
    Mutation
    Synonymized serine to prevent autoediting of ADAR…
    Promoter
    UBC
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pT7-NSP3SA11-P2A-C1C-EGFP

    Plasmid
    #220810
    Purpose
    Express separately the Rotavirus SA11 NSP3 protein and C1C-EGFP
    Depositor
    Insert
    Rotavirus SA11 NSP3-P2A-C1C-EGFP
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pLVX ALIX-GFP S718/721A

    Plasmid
    #138583
    Purpose
    Expresses fluorescent ALIX S718/721A in mammalian cells
    Depositor
    Insert
    ALIX (PDCD6IP Human)
    Use
    Lentiviral
    Tags
    GFP and h30
    Expression
    Mammalian
    Mutation
    changed serines 718 and 721 to alanines
    Promoter
    TREtight
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. AICSDP-58: NUP153-mEGFP

    Plasmid
    #114407
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human NUP153
    Insert
    NUP153 Homology Arms with mEGFP-linker (NUP153 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Sept. 5, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. AICSDP-35:AAVS1-mEGFP

    Plasmid
    #91565
    Purpose
    Homology arms and linker-mEGFP sequence for internal insertion of CAGGS-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP
    Insert
    AAVS1 Homology Arms with CAGGS driven mEGFP (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 7, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
  7. AICSDP-39:RAB5A-mEGFP

    Plasmid
    #107579
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human RAB5A
    Insert
    RAB5A Homology Arms with mEGFP-linker (RAB5A Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    May 9, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. AICSDP-82:SON-mEGFP

    Plasmid
    #133964
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human SON
    Insert
    SON Homology Arms with mEGFP-linker (SON Human)
    Use
    CRISPR
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Nov. 22, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
  9. lgp120+linker-pEGFP N1

    Plasmid
    #62963
    Purpose
    rat lgp120 + 10 amino acid linker cloned into pEGFP N1. The linker separates the targetting motif in lgp120 from EGFP.
    Depositor
    Insert
    lgp120 (Lamp1 Rat)
    Use
    Tags
    EGFP and linker
    Expression
    Mammalian
    Mutation
    has 2 amino acid mutations compared to the coding…
    Promoter
    CMV
    Available since
    May 28, 2015
    Availability
    Academic Institutions and Nonprofits only
  10. Cin85-3SH3-pCMV6-AN-GFP

    Plasmid
    #51137
    Purpose
    Express turbo GFP tagged three SH3 domains of Cin85 (truncation) in mammalian cells
    Insert
    Cin85 (SH3KBP1 Human)
    Use
    Tags
    TurboGFP
    Expression
    Mammalian
    Mutation
    contains 3 SH3 domains
    Promoter
    CMV
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. TDP-43 F147/149L GFP

    Plasmid
    #237545
    Purpose
    Expression of GFP-tag fused to mutant TDP-43 F147/19L
    Insert
    GFP-tagged TDP-43 F147/149L (TARDBP Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    TDP-43 F147L and F149L
    Promoter
    CMV
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
  12. Venus-30aa-Noxa-4E-pEGFP-C1

    Plasmid
    #177424
    Purpose
    To express Venus fused to Noxa-4E, with a longer linker sequence between compared to Addgene #166745.
    Depositor
    Insert
    Noxa, PMA-induced protein 1 (PMAIP1 Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
  13. mCerulean3-BclXL-ActA-pEGFP-C1

    Plasmid
    #177412
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    Insert
    Bcl-XL-ActA, BclX-acta
    Use
    Tags
    mCerulean3
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  14. mCerulean3-BclXL-Cb5-pEGFP-C1

    Plasmid
    #177414
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    Insert
    Bcl-XL-Cb5, BclX-cb5
    Use
    Tags
    mCerulean3
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  15. Venus-Bcl2-ActA-pEGFP-C1

    Plasmid
    #177419
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    Insert
    Bcl-2-ActA, Bcl2-acta, Bcl-acta
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  16. Venus-Bcl2-cb5-pEGFP-C1

    Plasmid
    #177422
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    Insert
    Bcl-2-Cb5, Bcl2-cb5, Bcl-cb5
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  17. pLenti-EF1a-GFP-miRE_shRen-Puro

    Plasmid
    #135683
    Purpose
    Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection
    Depositor
    Insert
    shRenilla
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  18. pLenti-CMV-GFP-miRE_shSCR-Puro

    Plasmid
    #135689
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Puromycin selection
    Depositor
    Insert
    shScrambled
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  19. pLenti-EF1a-GFP-miRE_shRen-Blast

    Plasmid
    #135682
    Purpose
    Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection
    Depositor
    Insert
    shRenilla
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  20. pLenti-CMV-GFP-miRE_shRen-Blast

    Plasmid
    #135684
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection
    Depositor
    Insert
    shRenilla
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2021
    Availability
    Academic Institutions and Nonprofits only
  21. pLenti-CMV-GFP-miRE_shTHUMPD3-Blast

    Plasmid
    #135690
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shROSA26 (control shRNA), Blasticidin selection
    Depositor
    Insert
    shRosa26
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  22. pLenti-CMV-GFP-miRE_shTHUMPD3-Puro

    Plasmid
    #135691
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shROSA26 (control shRNA), Puromycin selection
    Depositor
    Insert
    shRosa26
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  23. pLenti-CMV-GFP-miRE_shSCR-Blast

    Plasmid
    #135688
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Blasticidin selection
    Depositor
    Insert
    shScrambled
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  24. pLenti-CMV-GFP-miRE_shRen-Puro

    Plasmid
    #135685
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection
    Depositor
    Insert
    shRenilla
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  25. pSAH52 - [DDp | gfp | tbb-2 UTR]

    Plasmid
    #200328
    Purpose
    gfp BioPart for DD neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Insert
    [DDp | gfp | tbb-2 UTR]
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    WBGene00007304, ttr-39
    Available since
    June 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  26. pSAH48 - [ADLp | gfp | tbb-2 UTR]

    Plasmid
    #200324
    Purpose
    gfp BioPart for ADL neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Insert
    [ADLp | gfp | tbb-2 UTR]
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    WBGene00011644, T09B9.3
    Available since
    June 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  27. pAc5.1B-EGFP-DmSmaug_584-999-V5His6_C

    Plasmid
    #146003
    Purpose
    Insect Expression of DmSmaug_584-999
    Depositor
    Insert
    DmSmaug_584-999 (smg Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    5 silent mutations and a deletion of the AA 961-9…
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  28. pAc5.1B-EGFP-DmSmaug_763-999-V5His6_C

    Plasmid
    #146004
    Purpose
    Insect Expression of DmSmaug_763-999
    Depositor
    Insert
    DmSmaug_763-999 (smg Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    2 silent mutations and a deletion of the AA 961-9…
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  29. pAc5.1B-EGFP-DmSmaug-A642H-V5His6_C

    Plasmid
    #146005
    Purpose
    Insect Expression of DmSmaug-A642H
    Depositor
    Insert
    DmSmaug-A642H (smg Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    10 silent mutations and a deletion of the AA 961-…
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  30. pAc5.1B-EGFP-DmSmaug-K606AR609A-V5His6_C

    Plasmid
    #146007
    Purpose
    Insect Expression of DmSmaug-K606AR609A
    Depositor
    Insert
    DmSmaug-K606AR609A (smg Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    10 silent mutations and a deletion of the AA 961-…
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  31. pAc5.1B-EGFP-DmUPF2-siRNAres-V5His6_E

    Plasmid
    #146166
    Purpose
    Insect Expression of DmUPF2-siRNAres
    Depositor
    Insert
    DmUPF2-siRNAres (Upf2 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    3 non silent mutations: L1022P, V1097L and N1213S…
    Promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  32. pAc5.1B-EGFP-DmSmaug_1-762-V5His6_C

    Plasmid
    #146001
    Purpose
    Insect Expression of DmSmaug_1-762
    Depositor
    Insert
    DmSmaug_1-762 (smg Fly)
    Use
    Tags
    V5-6xHis
    Expression
    Insect
    Mutation
    NP_523987.1, 7 silent mutations compared to the s…
    Promoter
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  33. Venus-Bik-DD-pEGFP-C1

    Plasmid
    #177429
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.
    Depositor
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Threonine 33 and Serine 35 replaced with aspartic…
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  34. Venus-Bik-AA-pEGFP-C1

    Plasmid
    #177430
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with 2 sites of phosphorylation mutated to alanine. T33A and S35A mutations made to Addgene #166737.
    Depositor
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Threonine 33 and Serine 35 replaced with alanine
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  35. EGFPN2-hMyoX-223-3165

    Plasmid
    #47611
    Depositor
    Insert
    MyoX (MYO10 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    contains bp223-3165 of reference Genbank seq
    Promoter
    CMV
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
  36. PB CAG-R-bpA-3xSV40pA-R-td-sfGFP

    Plasmid
    #133579
    Purpose
    CAG tdGFP dre/rox reporter piggyBac transposon (bpA 3x SV40pA stop)
    Depositor
    Insert
    Tandem dimer superfolder GFP
    Use
    Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  37. p006-GFP-Logic-AND

    Plasmid
    #112237
    Purpose
    GFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant.
    Depositor
    Inserts
    GFP under control of doubly-repressible promoter
    GFP
    araC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    AND-gate and pLacI
    Available since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 851 - 900 of 6889 results