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pENTR_hhN-HA

Plasmid

#240225

Purpose

Gateway entry clone with N-terminal hedgehog (hhN) tagged with HA (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

hedgehog N-terminal fragment (hh Fly)

Use

Gateway shuttle vector

Tags

HA-spGFP11

Mutation

aa 1-257

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS140

Plasmid

#197141

Purpose

scCloneSelect C-to-T reporter encoding BC-C1 barcode (CMV promoter version)

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRS140

Plasmid

#197141

Purpose

scCloneSelect C-to-T reporter encoding BC-C1 barcode (CMV promoter version)

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRS191

Plasmid

#197140

Purpose

scCloneSelect C-to-T reporter encoding BC-C1 barcode (EF1a promoter version)

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

EF1a

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRS191

Plasmid

#197140

Purpose

scCloneSelect C-to-T reporter encoding BC-C1 barcode (EF1a promoter version)

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

EF1a

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pNM1325

Plasmid

#197144

Purpose

CAGp-Target-AID-2A-BlastR for PiggyBac genomic integration

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

SpCas9-PmCDA1-UGI

Use

CRISPR

Expression

Mammalian

Mutation

SpCas9(D10A)

Promoter

CAG

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pNM1325

Plasmid

#197144

Purpose

CAGp-Target-AID-2A-BlastR for PiggyBac genomic integration

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

SpCas9-PmCDA1-UGI

Use

CRISPR

Expression

Mammalian

Mutation

SpCas9(D10A)

Promoter

CAG

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CS-146

Plasmid

#197136

Purpose

CloneSelect Indel reporter encoding BC-del1 barcode

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-CS-146

Plasmid

#197136

Purpose

CloneSelect Indel reporter encoding BC-del1 barcode

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-CS-196

Plasmid

#197138

Purpose

CloneSelect C-to-T mCherry (M9A) ATG control reporter encoding BC-C1 barcode

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-CS-196

Plasmid

#197138

Purpose

CloneSelect C-to-T mCherry (M9A) ATG control reporter encoding BC-C1 barcode

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-CS-117

Plasmid

#197134

Purpose

CloneSelect C-to-T reporter encoding three target barcodes (3-input OR gate circuit)

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-CS-117

Plasmid

#197134

Purpose

CloneSelect C-to-T reporter encoding three target barcodes (3-input OR gate circuit)

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSI-218

Plasmid

#197139

Purpose

All-in-vector of SpCas9 gRNA cloning sites (BsmBI) and CMVp-Target-AID expression unit

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Type

Empty backbone

Use

CRISPR

Tags

SpCas9-PmCDA1-UGI

Expression

Mammalian

Mutation

SpCas9(D10A)

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSI-218

Plasmid

#197139

Purpose

All-in-vector of SpCas9 gRNA cloning sites (BsmBI) and CMVp-Target-AID expression unit

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Type

Empty backbone

Use

CRISPR

Tags

SpCas9-PmCDA1-UGI

Expression

Mammalian

Mutation

SpCas9(D10A)

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-CS-060

Plasmid

#197133

Purpose

CRISPRa reporter cloning backbone and MCP-p65-HSF1-2A-Puro expression

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Tags

EGFP and mCherry

Expression

Mammalian

Mutation

MCP(N55K)

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-CS-060

Plasmid

#197133

Purpose

CRISPRa reporter cloning backbone and MCP-p65-HSF1-2A-Puro expression

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Tags

EGFP and mCherry

Expression

Mammalian

Mutation

MCP(N55K)

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-CS-193

Plasmid

#197137

Purpose

CloneSelect C-to-T mCherry (M1V/M9A) reporter encoding BC-C1 barcode

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

mCherry(M1V/M9A)

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-CS-193

Plasmid

#197137

Purpose

CloneSelect C-to-T mCherry (M1V/M9A) reporter encoding BC-C1 barcode

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

mCherry(M1V/M9A)

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-CS-119

Plasmid

#197135

Purpose

CloneSelect A-to-G reporter encoding three target barcodes (3-input AND gate circuit)

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge