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pCMV_ABE8e–superNLS

Plasmid

#232429

Purpose

Expression plasmid for the ABE8e base editor with superNLS

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

ABE8e–superNLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV mRuby3-Galectin-3

Plasmid

#215375

Purpose

Expression of the endolysosomal damage marker Galectin-3 with an N-terminal mRuby3 tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

LGALS3 (LGALS3 Human)

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ucp1-Cre-miR122

Plasmid

#228408

Purpose

Expression of Cre recombinase from a rat Ucp1 enhancer-promoter. Contains miR122 target sequences that suppress expression in hepatocytes.

Depositor

Joerg Heeren

Article

Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

Inserts

cre recombinase with SV40 NLS
Cre recombinase with SV40 NLS

Use

AAV

Promoter

rat Ucp1 enhancer-promoter

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1-iPE-Plus

Plasmid

#214062

Purpose

inducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locus

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

PE-Plus

Expression

Mammalian

Promoter

TRE-tight

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pTetOFF SNCA-A53T

Plasmid

#215372

Purpose

All-in-one lentiviral construct for the dox repressible expression of the A53T mutant of alpha-synuclein.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

SNCA (SNCA Human)

Expression

Mammalian

Mutation

Ala53Thr

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-Sac-Abeta(M1-40)

Plasmid

#71876

Purpose

Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

Depositor

Dominic Walsh

Article

Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

Insert

Abeta(M1-40) (APP Human)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pmCherry-N1 GBP (GBP-mCherry)

Plasmid

#162879

Purpose

Expression in mammalian cells of GFP binding protein (GBP) tagged with mCherry

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

GFP Binding Protein

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pYLCRISPR/Cas9Pubi-H

Plasmid

#66187

Purpose

Expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

maize ubiquitin promoter (Pubi)

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

Plasmid

#216246

Purpose

Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

Cas9 and sgAAVS1.5

Use

CRISPR; Human safe harbor locus (a avs1) site-spe…

Expression

Mammalian

Promoter

CAG

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro

Plasmid

#216244

Purpose

Express AtAFB2.F74A mutant with mCherry tag for AID2

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

AtAFB2.F74A-mCherry

Use

CRISPR, TALEN, and Unspecified ; Human safe harbo…

Tags

mCherry

Expression

Mammalian

Mutation

F74A mutation

Promoter

CAG

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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hSyn-DIO-somBiPOLES-mCerulean

Plasmid

#154951

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Has Service

AAV Retrograde, AAV5, and AAV9

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Promoter

human synapsin

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-SNCA-A53T-mRuby3

Plasmid

#215379

Purpose

Expression of the A53T mutant of alpha-synuclein with a C-terminal mRuby3 tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

SNCA (SNCA Human)

Tags

mRuby3 tag

Expression

Mammalian

Mutation

Ala53Thr

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pU6_rd6_pegRNA_(PP7-C4-Q1)

Plasmid

#232433

Purpose

pegRNA with optimized 3' modifications to correct the rd6 mutation

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

3'-PP7-tagged pegRNA for rd6 correction driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pU6_HEK3+1T>A_(PP7-C4-Q1)

Plasmid

#232437

Purpose

pegRNA with optimized 3' modifications to facilitate a +1 T to A prime edit in the HEK3 locus

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-Osp.gRNA-MS2in

Plasmid

#229772

Purpose

Contains the U6 promoter and an optimized gRNA backbone inserted with two copies of the MS2 stem loop.

Depositor

Yujia Cai

Article

Ling et al Nat Nanotechnol. 2025 Apr;20(4):543-553.

Insert

gRNA cassette with two MS2 stem loops

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-sgCTRL

Plasmid

#209750

Purpose

Lentiviral transfer plasmid to express Cas9 and a control, non-specific gRNA (does not target any human gene)

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

Cas9

Use

Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCG_IFITM3

Plasmid

#179957

Purpose

Expression vector for human IFITM3.

Depositor

Frank Kirchhoff, Konstantin Sparrer

Article

Prelli Bozzo et al Nat Commun. 2021 Jul 28;12(1):4584.

Insert

IFITM3 (IFITM3 Human)

Expression

Mammalian

Available Since

Feb. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pW17

Plasmid

#158207

Purpose

2lox landing pad plasmid with mariner transposon and transposase used for CRAGE

Depositor

Yasuo Yoshikuni

Article

Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

Insert

loxP/Cre/KmR/Lox5171/Mariner IR oriT/transposase

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pU6_HEK3_nsgRNA(PP7)

Plasmid

#232444

Purpose

nsgRNA for PE3 facilitation of a +1 T to A prime edit on the HEK3 locus, contains a PP7 in the scaffold tetraloop

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

PP7-tagged nicking gRNA (PE3) for HEK3+1t>a edit driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pPtGE34

Plasmid

#107932

Purpose

Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

Depositor

David Edgell

Article

Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

Inserts

OriT
40SRPS8 Promoter
ShBle
40SRPS8 Terminator
Cen6-ArsH4-His3

Use

CRISPR and Synthetic Biology; Episomal vector for…

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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