We narrowed to 9,805 results for: wus

1. pCnCas12f1HS_sgRNA_MS13_empty

   Plasmid

   #204999

   Purpose

   CnCas12f1 with MS13 sgRNA site for Human cell genome editing

   Depositor

   Quanjiang Ji

   Article

   Su et al Nat Chem Biol. 2024 Feb;20(2):180-189. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF1a_SNAI2_P2A_Hygro_Barcode

   Plasmid

   #120478

   Purpose

   Barcoded lentiviral vector to express SNAI2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   SNAI2 (SNAI2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lsg-GBA-1 + 2

   Plasmid

   #199276

   Purpose

   nicking sgRNA to induce or correct GBA (c. 1226 A > G) mutation using PE3

   Depositor

   Ting Zhou

   Article

   Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

   Insert

   GBA nick
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Dnase1L3 pIRES-DsRed

   Plasmid

   #98845

   Purpose

   Expresses Human Dnase1L3 in mammalian cells along with DsRed

   Depositor

   Peter Keyel

   Article

   Shi et al Front Immunol. 2017 May 8;8:522. doi: 10

   Insert

   Dnase1L3 (DNASE1L3 Human)
   

   Use

   Tags

   IRES DsRed

   Expression

   Mammalian

   Mutation

   long isoform, wild type

   Promoter

   CMV

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn1-tTA

   Plasmid

   #99119

   Purpose

   An AAV genome that expresses tTA from the hSyn1 promoter

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   tTA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   *(see below)

   Promoter

   hSyn1

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CASI-Cas9C-P2A-turboGFP

   Plasmid

   #80942

   Purpose

   Expresses Cas9C in mammalian cells; co-expresses turboGFP.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9C
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. BFP-Dcp1a

   Plasmid

   #153973

   Purpose

   Fluorescent protein fused to mRNA decapping enzyme 1a

   Depositor

   Gia Voeltz

   Article

   Lee et al Science. 2020 Jan 31;367(6477). pii: 367

   Insert

   mRNA decapping enzyme 1a (DCP1A Human)
   

   Use

   Tags

   mTagBFP-c1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MTK2_023

   Plasmid

   #123715

   Purpose

   Encodes pCMV as a Type 2 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pCMV
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLck-mVenus-C1

   Plasmid

   #84337

   Purpose

   Expresses a mVenus tagged with a membrane localization sequence

   Depositor

   Dorus Gadella

   Article

   van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

   Insert

   Lck (MGCVCSSNPELPVAT)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCambia-PUP-IT

    Plasmid

    #186478

    Purpose

    Expresses POI-PUP-IT fusion in Plants

    Depositor

    Jen Sheen

    Article

    Ye et al Nature. 2022 Sep 14. pii: 10.1038/s41586

    Insert

    FLAG-Pup(E)
    

    Use

    Tags

    Triple Flag Tag

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX307 RFP

    Plasmid

    #163976

    Purpose

    Mammalian expression vector encoding RFP (for example, to normalize plasmid-based protein expression)

    Depositor

    Daniel Bachovchin

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    RFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn1-mRuby2

    Plasmid

    #99126

    Purpose

    An AAV genome that expresses the fluorescent protein mRuby2 from the hSyn1 promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-TRE-mRuby2

    Plasmid

    #99114

    Purpose

    An AAV genome with tet-inducible expression of the fluorescent protein mRuby2

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ142-ZmUbi-tRNA

    Plasmid

    #158578

    Purpose

    Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 and pYPQ132-tRNA2.0; assembly of 2 gRNAs

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin 1

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRIP3-mCherry

    Plasmid

    #61386

    Purpose

    Expresses RIP3-mCherry in mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    RIP3 (RIPK3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-CAG-miRFP680-P2A-GFP

    Plasmid

    #197164

    Purpose

    Expresses the protein of miRFP680-P2A-GFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    miRFP680-P2A-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-cyto-KB1691-Nluc

    Plasmid

    #204326

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    cytosolic KB1691-Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pAAV-ADP-MCS-FLAG

    Plasmid

    #192360

    Purpose

    Adipocyte-specific overexpression of genes

    Depositor

    Yifu Qiu

    Article

    Xue et al Cell Metab. 2022 Sep 6;34(9):1325-1341.e

    Type

    Empty backbone

    Use

    AAV

    Tags

    3xflag

    Expression

    Mammalian

    Mutation

    Promoter

    adiponectin promoter

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-TRE-mTurquoise2

    Plasmid

    #99113

    Purpose

    An AAV genome with tet-inducible expression of the fluorescent protein mTurquoise2

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mTurquoise2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. mpx:UtrCH-GFP

    Plasmid

    #45247

    Depositor

    Anna Huttenlocher

    Article

    Yoo et al Dev Cell. 2010 Feb 16. 18(2):226-36.

    Insert

    utrophin (UTRN Human)
    

    Use

    Zebrafish expression

    Tags

    EGFP

    Expression

    Mutation

    contains amino acids 1-261 of NP_009055.2; Q236R

    Promoter

    mpx

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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