We narrowed to 10,378 results for: mcs

Showing: 3641 - 3660 of 10378 results

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pAF0034 Ef1a-Kp03-2a-EGFP

Plasmid

#193461

Purpose

Expression of Kp03 recombinase from Ef1a promoter

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Kp03

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAF0035 Ef1a-Nm60-2a-EGFP

Plasmid

#193464

Purpose

Expression of Nm60 recombinase from Ef1a promoter

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Nm60

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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TUBB5-Halo

Plasmid

#64691

Purpose

Expresses beta-tubulin labeled with Halo-tag

Depositor

Yasushi Okada

Article

Uno et al Nat Chem. 2014 Aug;6(8):681-9. doi: 10.1

Insert

TUBB

Tags

Halo-tag

Expression

Mammalian

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pAF0084 Ef1a-Cp36-2a-EGFP

Plasmid

#193462

Purpose

Expression of Cp36 recombinase from Ef1a promoter

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Cp36

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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(559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

Plasmid

#163024

Purpose

Expression of CRISPRi with U6-gRNA (Bsa1 sites)

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

AAV

Tags

KRAB, SV40 polyA, and myc NLS

Expression

Mammalian

Promoter

hEF-1a

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAF0362 Ef1a-Cp36_attP-Puro-p2a-mCherry donor

Plasmid

#193469

Purpose

Cp36 Donor with promoter for mcherry and puro expression

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Cp36_attP

Expression

Mammalian

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42-T17N

Plasmid

#12976

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Cdc42 DN (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T17N Dominant negative

Promoter

CMV

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-NanoLuc-Puromycin

Plasmid

#167209

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

NanoLuc

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42-Q61L

Plasmid

#12986

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Cdc42 constitutively active (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q61L Constitutively active

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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ND6-DdCBE-left side TALE

Plasmid

#157842

Purpose

expresses left-side TALE–DddAtox half of ND6-DdCBE in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–SOD2 3'UTR

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-4

Plasmid

#172770

Purpose

Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-4

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1-Q61L

Plasmid

#12981

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Rac1 constitutively active (RAC1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q61L Constitutively active

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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LADL Anchor

Plasmid

#127664

Purpose

Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

dCas9-CIBN-2A-Puro

Tags

3XFLAG

Expression

Mammalian

Mutation

Cas9 (D10A,H840A) mutant

Promoter

EF1a

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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ND4 right TALE–G1397 DddAtox C

Plasmid

#158097

Purpose

expresses the right-side ND4 TALE–DddAtox half in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

COX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA tox-C–1xUGI

Expression

Mammalian

Mutation

WT

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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ND4 left TALE–G1397 DddAtox N

Plasmid

#158094

Purpose

expresses the right-side ND4 TALE–DddAtox half in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

SOD2 MTS–3xHA–ND4 left TALE–G1397 DddAtox-N–1xUGI

Expression

Mammalian

Mutation

WT

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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HA-TRIM28deltaLinker

Plasmid

#124958

Purpose

Mammalian expression of Human TRIM28 with deleted Linker region between CC and PHD domains

Depositor

Michelle Barton

Article

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of linker region between CC domain and P…

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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ND4 right TALE–G1333 DddAtox C

Plasmid

#158095

Purpose

expresses the right-side ND4 TALE–DddAtox half in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

COX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-C–1xUGI

Expression

Mammalian

Mutation

WT

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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ND4 left TALE–G1333 DddAtox N

Plasmid

#158093

Purpose

expresses the left-side ND4 TALE–DddAtox half in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

SOD2 MTS–3xHA–ND4 left TALE–G1333 DddAtox-N–1xUGI

Expression

Mammalian

Mutation

WT

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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3541 pMIG Bcl-XL

Plasmid

#8790

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bcl-XL (BCL2L1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42-wt

Plasmid

#12975

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Cdc42 (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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