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pPICZ-aC-MSVU-BZ

Plasmid

#160426

Purpose

Expression of c-luciferase from sp. Maristella sp. SVU (Belize)

Depositor

Todd Oakley

Article

Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

Insert

luciferase

Use

Luciferase

Tags

6x Histidine tag, Yeast alpha secretion factor, c…

Expression

Yeast

Mutation

See depositor comments below

Promoter

AOX

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pNB1-LOX

Plasmid

#154058

Purpose

Level 1, Position 3 Golden Gate vector. ZmUbi-5'UTR:loxP-GUS-nosT-loxP-NAM-B1-nosT

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

LoxP-flanked GUS and TtNAM-B1

Use

Synthetic Biology

Expression

Bacterial

Mutation

The TtNAM-B1 gene sequence was domesticated to re…

Promoter

ZmUbi

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_ABI1-2/2

Plasmid

#91380

Purpose

Protein expression and purification of human SH3 domain construct ABI1-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ABI1-2/2 (ABI1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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csd6_1-330/pET28b(+) N-tag

Plasmid

#71350

Purpose

csd6, construct:1-330, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 )

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
KHBD00450

Plasmid

#39563

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6103 (CrebB Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00053

Plasmid

#34215

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6103 (CrebB Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA-c/o NL4-3 Env delta CT

Plasmid

#139285

Purpose

HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

codon optimized HIV-1 Env delta cytoplasmic tail

Expression

Mammalian

Mutation

deletion of cytoplasmic tail after residue 710

Promoter

CMV IE

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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PB-GG-OMKSL-PGK-Puro

Plasmid

#102894

Purpose

PiggyBac transposon system construct with 10 concatenated U6 promoter driven transcriptional cassettes targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Contains PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

OMKSL-gRNAs-PGK-Puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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GG-EBNA-OMKSL-PP

Plasmid

#102899

Purpose

EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

OMKSL-gRNAS-PGK-puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pIntegrase_4

Plasmid

#60576

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_4

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_2

Plasmid

#60574

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_2

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_7

Plasmid

#60578

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_7

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_12

Plasmid

#60583

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_12

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_13

Plasmid

#60584

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_13

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_11

Plasmid

#60582

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_11

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCast

Plasmid

#200169

Purpose

E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

Depositor

Yong-Su Jin

Article

Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pIntegrase_8

Plasmid

#60579

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_8

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pReporter_8

Plasmid

#60568

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_8

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCIG3 N

Plasmid

#132941

Purpose

MLV packaging plasmid for N-tropic MLV gag-pol expression

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

N-tropic MLV gag-pol

Use

Retroviral

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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ER_K20

Plasmid

#133861

Purpose

Expression of ER-targeted Ribosome stalling reporter

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

ER_K20

Tags

EGFP and RFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

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