We narrowed to 104,063 results for: nin

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pU6-sp-sgRNA-RNF2_+41nick

Plasmid

#135958

Purpose

S. pyogenes sgRNA for +41 nick in RNF2

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_+41 nicking sgRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-NTRK3

Plasmid

#23901

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NTRK3 (NTRK3 Human)

Use

Gateway Cloning

Mutation

F441L and L653P relative to NM_001012338.3

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pAd NeuroD-I-nGFP

Plasmid

#19414

Depositor

Douglas Melton

Article

Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

Insert

neurogenic differentiation 1 (Neurod1 Mouse)

Use

Adenoviral and Gateway Cloning

Tags

GFP and IRES

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
AAVS1 pegRNA2 with trimmed attP

Plasmid

#222347

Purpose

pegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installation

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

AAVS1 attP-installation pegRNA2

Use

CRISPR

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSLIK-GFP

Plasmid

#66844

Purpose

Tet-inducible expression of GFP in mammalian cells

Depositor

Christian Metallo

Article

Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-CA-mDia1

Plasmid

#45583

Depositor

Klaus Hahn, Ronen Zaidel-Bar

Article

Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

Insert

Constitutively active mDia1 (CA-mDia1) (Diaph1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Insert mDia1 contains mutations V161D and N165D i…

Promoter

CMV

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pLenti6.3/TO/CD9-GFP

Plasmid

#104402

Purpose

Expresses CD9 with mEmerald tag (NOT GFP) at the N terminal

Depositor

Jens Gruber

Article

Boker et al Mol Ther. 2018 Feb 7;26(2):634-647. doi:

Insert

CD9 (CD9 Human)

Use

Lentiviral

Tags

mEmerald

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLGB30

Plasmid

#126620

Purpose

Suicide vector for allelic replacement in Bacteroides, tetracycline selection and rhamnose-inducible ss-Bfe1 counterselection

Depositor

Laurie Comstock

Article

Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

Type

Empty backbone

Use

Allellic exchange in bacteria

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

Plasmid

#138460

Purpose

lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

MCP-KRAB-IRES-zsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-N LMP2A

Plasmid

#37963

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

LMP2A (LMP-2A H. Herpesvirus 4 (EBV))

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pBAMD1-4

Plasmid

#61565

Purpose

Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Available Since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only

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MP4

Plasmid

#69652

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA

Expression

Bacterial

Promoter

E. coli PBAD

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-HA-EIF3S3

Plasmid

#22545

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

EIF3S3(1-348aa) (EIF3H Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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R4pGWB601_UBQ10p-Turbo-YFP-NLS

Plasmid

#127368

Purpose

Binary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plants

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Tags

GS linker, NLS, V5, and YFP

Expression

Plant

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

Arabidopsis UBQ10 promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PRKAA1

Plasmid

#20595

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PRKAA1 (PRKAA1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pFRT/FLAG/HA-DEST PUM2

Plasmid

#40292

Depositor

Thomas Tuschl

Article

Hafner et al Cell. 2010 Apr 2;141(1):129-41.

Insert

Pumilio homolog 2 (PUM2 Human)

Tags

FLAG-HA

Expression

Mammalian

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-nv5-ER beta

Plasmid

#22770

Depositor

Donald McDonnell

Article

Wittmann et al Cancer Res. 2007 Oct 1. 67(19):9549-60.

Insert

estrogen receptor beta (ESR2 Human)

Tags

v5

Expression

Mammalian

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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PEA1-Puro

Plasmid

#171991

Purpose

Delivers all prime editing nickase components in a single, puromycin selectable plasmid

Depositor

Paul Thomas

Article

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Expression

Mammalian

Promoter

CMV for Cas9, U6 for gRNAs

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-PS9

Plasmid

#177944

Purpose

Transient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

eGFP (ORF1ab )

Expression

Mammalian

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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3341 pcDNA3 Bcl-2 T69A S70A S87A

Plasmid

#13340

Depositor

Stanley Korsmeyer

Article

Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

Insert

Bcl-2 (BCL2 Human)

Expression

Mammalian

Mutation

changed Threonine 69 to Alanine; changed Serine 7…

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only