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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 50,536 results for: kan

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Showing: 3701 - 3720 of 50536 results

  2. M_ST1n_VPR

    Plasmid
    #63799
    Purpose
    ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector
    Depositor
    George Church
    Publication
    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ST1-dCas9-VPR
    Use
    CRISPR and Synthetic Biology
    Tags
    VPR (VP64-p65-Rta)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    63799-plasmid-map-sequence-id-106659

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  3. pcDNA3-AKAR4-Kras

    Plasmid
    #61621
    Purpose
    Non-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activity
    Depositor
    Jin Zhang
    Publication
    Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AKAR4-Kras
    Tags
    C-terminal targeting sequence from Kras, Cerulean…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61621-plasmid-map-sequence-id-100422

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  5. TRUPATH Triple Gaq

    Plasmid
    #196058
    Purpose
    Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAlphaQ-RLuc8 (GNAQ Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196058-plasmid-map-sequence-id-385868

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  6. pcDNA3.1-hNurr1-NE

    Plasmid
    #102363
    Purpose
    Overexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cells
    Depositor
    Shu Leong Ho
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
    Tags
    NE
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    102363-plasmid-map-sequence-id-200857

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  12. pCRISPEY-GAL-Kan-ADE2

    Plasmid
    #232105
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Jeffrey Lewis
    Publication
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232105-plasmid-map-sequence-id-462309

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  13. pCas9-EcRT-GAL-Kan

    Plasmid
    #232092
    Purpose
    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.
    Depositor
    Jeffrey Lewis
    Publication
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Insert
    SpCas9 and Ec86 reverse transcriptase
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Ec86 reverse transcriptase is codon optimized for…
    Promoter
    GAL1-GAL10
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232092-plasmid-map-sequence-id-459849

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  16. DNMT3A-dCas9

    Plasmid
    #100090
    Purpose
    DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNMT3A (DNMT3A Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-DNMT3A-dCas9-NLS
    Expression
    Mammalian
    Mutation
    includes aa 602-912
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100090-plasmid-map-sequence-id-197562

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  17. pcDNA3-GFP-MT2A

    Plasmid
    #11613
    Depositor
    Steven Johnson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    metallothionein 2A (MT2A Human)
    Tags
    GFP
    Expression
    Mammalian
    Available since
    July 18, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11613-plasmid-map-sequence-id-365342

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  19. pcDNA3-Ii-OVA.I+II

    Plasmid
    #64596
    Purpose
    Expression of invariant chain fused to chicken ovalbumin 242-353 amino acids as model antigen for study of MHC class II presentation
    Depositor
    Sandra Diebold, Martin Zenke
    Publication
    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.
    Insert
    Ovalbumin
    Tags
    Invariant chain
    Expression
    Mammalian
    Mutation
    Only amino acids 242-353 (class I and class II ep…
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64596-plasmid-map-sequence-id-118017

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Showing: 3701 - 3720 of 50536 results