Addgene

We narrowed to 13,990 results for: lif

Showing: 7441 - 7480 of 13990 results

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pNH040

Plasmid

#42152

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

GFP

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pNH050

Plasmid

#42156

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

CFP-RT-CFP

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pDR-AmTrac

Plasmid

#47770

Purpose

expresses AmTrac in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac

Use

Tags

mcpGFP

Expression

Yeast

Mutation

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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p205 pCMV-cre-del

Plasmid

#8393

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

deletion

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

1 kb EcoRI to HindIII deletion of pBS185.

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pDR-AmTrac1;2

Plasmid

#47765

Purpose

expresses AmTrac1;2 in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac1;2

Use

Tags

mcpGFP

Expression

Yeast

Mutation

Promoter

PMA

Available since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only
pDR-AmTrac-LS

Plasmid

#47767

Purpose

expresses AmTrac-LS in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac-LS

Use

Tags

mcpGFP

Expression

Yeast

Mutation

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
p230 pCMVe-betaAc-luc

Plasmid

#8391

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CMVe-betaAc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2005

Availability

Academic Institutions and Nonprofits only
pDR-meptrac

Plasmid

#47768

Purpose

expresses MepTrac in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

meptrac

Use

Tags

mcpGFP

Expression

Yeast

Mutation

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pDR-AmTrac-IS

Plasmid

#47766

Purpose

expresses AmTrac-IS in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac1;2

Use

Tags

mcpGFP

Expression

Yeast

Mutation

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pNH043

Plasmid

#42155

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX2TK E7 DLYC

Plasmid

#13672

Depositor

Karl Munger

Article

Jones et al Genes Dev. 1997 Aug 15. 11(16):2101-11.

Insert

HPV 16 E7 del DLYC (E7 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

del aa 21-24

Promoter

Available since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-eIF4E

Plasmid

#232336

Purpose

Expresses human eIF4E (ENST00000450253.7, NM_001968.5) in mammalian cells

Depositor

Quanfu Ma

Insert

eIF4E (EIF4E Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRsetB-RoTq-On

Plasmid

#234465

Purpose

Bacterial expression vector for RoTq-On

Depositor

Gary Yellen

Article

Rosen et al Proc Natl Acad Sci U S A. 2025 May 13;12

Insert

RoTq-On

Use

Tags

7xHis

Expression

Bacterial

Mutation

Promoter

Available since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH1364

Plasmid

#223681

Purpose

AP1_RE-miniCMV-GFP-pEF-G418R

Depositor

Gaelen Hess

Article

Liila-Fogarty et al ACS Synth Biol. 2024 Oct 19. doi: 10.102

Insert

GFP and G418 Resistance

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-5

Plasmid

#221491

Purpose

NKX2-5 lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-5 (NKX2-5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1

Plasmid

#221472

Purpose

Gateway cloning of NKX2-1 short isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL.004

Plasmid

#184969

Purpose

Negative control, empty integrating inducible casette

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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3xTRE DREaMR 3p3DsR

Plasmid

#182206

Purpose

3xTRE Tet-On cassette with 3xp3 DsRed maker for generating knockins with doxycycline-inducible rescue

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

rTetR-3XP3-DsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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1xTRE DREaMR 3p3DsR

Plasmid

#182204

Purpose

1xTRE Tet-On cassette with 3xp3 DsRed marker for generating knockins with doxycycline-inducible rescue.

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

rTetR-3XP3-DsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-c5X-poly(alanylglycine)

Plasmid

#67003

Purpose

Expresses malE-poly(alanylglycine) in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-poly(alanylglycine)

Use

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-poly-EAK9

Plasmid

#67010

Purpose

Expresses malE-poly-EAK9 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-poly-EAK9

Use

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pGC461

Plasmid

#71363

Purpose

distal tip cell expression of DAF-16 in C. elegans

Depositor

Jane Hubbard

Article

Michaelson et al Development. 2010 Feb;137(4):671-80. doi

Insert

daf-16 (daf-16 Nematode)

Use

Tags

GFP(65C)

Expression

Worm

Mutation

Promoter

Blelloch et al., 1999; Mathies et al., 2003

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pDR-AmTrac-GS

Plasmid

#47769

Purpose

expresses AmTrac-GS in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac-GS

Use

Tags

mcpGFP

Expression

Yeast

Mutation

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pNH052

Plasmid

#42158

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

YFP-RT-YFP

Use

Tags

Expression

Mutation

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pNH054

Plasmid

#42160

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1-RT-mTFP1

Use

Tags

Expression

Mutation

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pNH039

Plasmid

#42151

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

CFP

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pNH058

Plasmid

#42161

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

TAP Tag::mTFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAdEasy-2

Plasmid

#16401

Purpose

Adenoviral vector for use in AdEasy System, for use with large inserts.

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21b-P35

Plasmid

#11808

Depositor

Guy Salvesen

Article

Riedl et al Biochemistry. 2001 Nov 6. 40(44):13274-8

Insert

P35

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(-)-qmTQ2-cAMP

Plasmid

#225250

Purpose

mammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-cAMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1_3XFlag

Plasmid

#182494

Purpose

Empty plasmid containing Flag tag for C-terminal tagging

Depositor

Paolo Comoglio

Article

Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

Insert

3xFlag with 5 Glycolic spacer

Use

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p212 pCMV-EGFP/RFP

Plasmid

#8389

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

floxed EGFP

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-hCDC6p-Venus-puro

Plasmid

#212666

Purpose

Express Venus under CDC6 promoter

Depositor

Tobias Meyer, Yumi Konagaya

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

hCDC6p-Venus

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hCDC6p

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
35S:RUBY

Plasmid

#160908

Purpose

RUBY under the control of CaMV 35S promoter/marker for transformation

Depositor

Yunde Zhao

Article

He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

Insert

RUBY and CaMV 35S promoter

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-scFv-GCN4-sfGFP-GB1-dWPRE

Plasmid

#60907

Purpose

The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE

Plasmid

#60906

Purpose

The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
RpHLuorin2

Plasmid

#180227

Purpose

for bacterial expression of recombinant ratiometric pHluorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2

Use

Tags

His-tag followed by thrombin cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-MBP-TEV

Plasmid

#69929

Purpose

bacterial expression of MBP-TEV n-terminal fusions

Depositor

Zita Balklava, Thomas Wassmer

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

MBP and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Jan. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO_CalR_HaloTag9_KDEL

Plasmid

#175540

Purpose

Expression of HaloTag9 in the endoplasmid reticulum of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CalR-HaloTag9-KDEL

Use

Tags

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET263-pUC57 24xMS2V7

Plasmid

#140705

Purpose

MS2V7 cloning

Depositor

Evelina Tutucci, Robert Singer

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V7

Use

Cloning vector

Tags

Expression

Mutation

24 MS2 loops V7, high-affinity C-variant, inter-s…

Promoter

None

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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