We narrowed to 35,837 results for: CaS

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AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E)

Plasmid

#120293

Purpose

AAV Vector for expression of N-terminal SpyCas9 fragment with Intein and a U6-driven F+E gRNA scaffold

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

N-terminal fragment of SpyCas9

Use

AAV and CRISPR

Tags

SV40-NLS and split-intein

Expression

Mammalian

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pPW3754 : pUC-SpCas9_gRNA-HsIRE1-Cterm

Plasmid

#185676

Purpose

SpCas9 and gRNA targeting the C-terminus of HsIRE1

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 gRNA (ERN1 Human)

Use

CRISPR

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only
pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

Plasmid

#71829

Purpose

Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control

Depositor

Vlatka Zoldos

Article

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

BACH2-sgRNA8 (BACH2 Synthetic, S. pyogenes, Human)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9; E756A inactiva…

Promoter

U6

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-CASPR/Neurexin IV [K65/35R]

Plasmid

#177445

Purpose

Mammalian Expression Plasmid of anti-CASPR/Neurexin IV (Rat). Derived from hybridoma K65/35.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-CASPR/Neurexin IV (Rattus norvegicus) recombinant mouse monoclonal antibody (Cntnap1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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XLone-BSD Cas13d P2A mCherry

Plasmid

#155183

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d and mCherry

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Insert

CasRx

Use

Unspecified; Piggybac transposon

Tags

NLS and P2A mCherry

Expression

Mammalian

Promoter

TRE3G

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-Caspase-1 (C285A)

Plasmid

#131353

Purpose

Expresses Caspase-1 C285A

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

Caspase-1 (C285A) (Casp1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Caspase-1 (C285A)

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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PET-eSpCas9-plus-NLS-6xHis

Plasmid

#207380

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

eSpCas9-plus

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, R1060A, amino acids 1005-1013 replaced wit…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-E1347A-nCas9(D10A)-UGI

Plasmid

#187410

Purpose

DddA E1347A variant

Depositor

Jin-Soo Kim

Article

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA E1347A variant

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-cGAS g2 Cas9-T2A-mCherry

Plasmid

#164253

Purpose

Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgcGAS

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC1124 - pAAV MeCP2 SpCas9(D10A)

Plasmid

#112719

Purpose

An AAV vector that expresses SpCas9 nickase under a neuronal cell promoter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

SpCas9

Use

AAV

Mutation

D10A

Promoter

Mecp2

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9

Plasmid

#186699

Purpose

Plasmid encoding PRDM9dC-Cas9 fusion under CMV promoter

Depositor

Jennifer Doudna

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

Insert

PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9 (PRDM9 Human)

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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PGK+ plasmid with onboard mCherry cassette

Plasmid

#169745

Purpose

PGK+ plasmid that also encodes separate mCherry cassette. mCherry is used to monitor plasmid dosage.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

PGK-tetO2

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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p2T-cmv-SpCas9-TadCBEa-BlastR

Plasmid

#193847

Purpose

Express TadCBEa in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEa

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-PspCas13b-non-targeting (pJK5)

Plasmid

#229052

Purpose

Cloning backbone for PspCas13b crRNA (U6 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-AAAAA-nCas9(D10A)-UGI

Plasmid

#187409

Purpose

DddA AAAAA variant

Depositor

Jin-Soo Kim

Article

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA AAAAA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pC011 LwCas13a locus with targeting spacer

Plasmid

#91903

Purpose

LwCas13a locus into pACYC184 with targeting spacer

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LwCas13a with targeting spacer

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 16, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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Tol2-LyzC-Cas9-pA-Cry-GFP

Plasmid

#168239

Purpose

"neutrophil specific cas9 expression in zebrafish with green lens"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Cas9

Use

Zebrafish expression

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pGinB-8X(GGS)-dCas9-FLAG-NLS

Plasmid

#81205

Purpose

CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

pGinB-8X(GGS)-dCas9-FLAG-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pmU1-CasRx-non-targeting (pKW83)

Plasmid

#229051

Purpose

Cloning backbone for CasRx crRNA (Mouse U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pZac2.1 SV40-CMV-SaCas9-3xNLS

Plasmid

#78601

Purpose

AAV vector containing SaCas9

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

SV40-CMV-SaCas9-3xNLS

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Promoter

SV40, CMV promoters

Available Since

Dec. 2, 2017

Availability

Academic Institutions and Nonprofits only