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pDx_mScarlet-I3

Plasmid

#189757

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLS-SV40-mP-EGFP

Plasmid

#137724

Purpose

Lentivirus based EGFP expression vector

Depositor

Nadav Ahituv

Article

Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

Insert

SV40 enhancer

Use

Lentiviral

Expression

Mammalian

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ356 - MCS(cxTi10816, IV)

Plasmid

#34871

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

cxTi10816 targeting

Use

Worm targeting

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pY094

Plasmid

#84743

Purpose

Expresses huAsCpf1-T2A-GFP and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
GFP

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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SIRT1.1

Plasmid

#13735

Depositor

John Denu

Article

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

SIRT1 (SIRT1 Human)

Tags

His

Expression

Bacterial

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV d14GFP Donor

Plasmid

#31475

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d14GFP

Use

Lentiviral

Mutation

Deleted 14 amino acids on the c terminus of eGFP

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FlipCherry-T2A-GFP

Plasmid

#124436

Purpose

Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipCherry-T2A-GFP

Expression

Mammalian

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pmiRFP703-N1

Plasmid

#79988

Purpose

Monomeric near-infrared fluorescent protein miRFP703 in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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hAur-A

Plasmid

#8510

Depositor

Joan Ruderman

Article

Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

Insert

hAur-A (AURKA Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pAS1NB c Rosella I

Plasmid

#71245

Purpose

a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.

Depositor

Mark Prescott

Article

Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

Insert

Rosella

Expression

Yeast

Promoter

PGK1

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Sox2-IP

Plasmid

#13459

Depositor

Shinya Yamanaka

Article

Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

Insert

Sox2 (Sox2 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pDuet TDP43 WT

Plasmid

#27462

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Coexpression

Tags

GST

Expression

Bacterial

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pHJ320

Plasmid

#20671

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Moesin (MSN Human)

Tags

GFP

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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pMBIS

Plasmid

#17817

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA-SADB19L

Plasmid

#32632

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

SADB19L

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pCMVTNT PINK1 N-myc

Plasmid

#13313

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Tags

myc

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pCas9-sgAAVS1-1

Plasmid

#129726

Purpose

Expressing SpCas9 and sgAAVS1-1

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

SpCas9 and sgAAVS1-1

Use

CRISPR

Tags

P2A-Puro

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pmVenus(L68V)-mTurquoise2

Plasmid

#60493

Purpose

Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

SYFP2-mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-TCF1

Plasmid

#40620

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Insert

TCF7 (TCF7 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pPAmCherry-a-tubulin

Plasmid

#31930

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Tags

a-tubulin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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