We narrowed to 51,260 results for: des.1

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pcDNA3puro-mGFP-FMR1iso1-N1(1-445)

Plasmid

#225464

Purpose

Express mEGFP-fusion protein of FMR1 fragment (1-445)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FMR1 (FMR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

N1 fragment (1-445)

Promoter

CMV

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FMR1iso1-N2(1-491)

Plasmid

#225465

Purpose

Express mEGFP-fusion protein of FMR1 fragment (1-491)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FMR1 (FMR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

N2 fragment (1-491)

Promoter

CMV

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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TL1 alphaL_DdCBE 1 (left, DddA11-C)

Plasmid

#221996

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alphaL-1 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A | DddA: T1413I

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pGEM-601[insert(+1) TA-rich]

Plasmid

#221196

Purpose

601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

601[insert(+1)TA-rich]

Use

Unspecified

Mutation

601 has an additional mutation added 22 bp from d…

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF1b-His8-TEV-PI31-1-151

Plasmid

#202593

Purpose

Bacterial expression of HsPI31-1-151

Depositor

Brenda Schulman

Article

Kraus et al EMBO Rep. 2023 Aug 3;24(8):e56399. doi:

Insert

PI31 (PSMF1 Human)

Tags

8xHis, TEV

Expression

Bacterial

Mutation

aa 1-151 only

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-His6-E3(1)-CUbo-TwinStrep

Plasmid

#212712

Purpose

Expression of His6-E3(1)-CUbo-TwinStrep in insect cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Tags

6xHis and CUbo-TwinStrep

Expression

Bacterial

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Myc-E3(1)-CUbo

Plasmid

#212799

Purpose

Constitutive/Doxycycline-inducible expression of Myc-E3(1)-CUbo in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Tags

CUbo and Myc

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-Best1-nt.Txnip(C247S)(1-320aa)

Plasmid

#206352

Purpose

AAV plasmid expressing deltion and mutant TXNIP in retinal pigment epithelium

Depositor

Connie Cepko

Article

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Txnip (Txnip Mouse)

Use

AAV

Mutation

(1-320aa), C247S

Promoter

human Best1

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-RedO-nt.Txnip(C247S)(1-301aa)

Plasmid

#206354

Purpose

AAV plasmid expressing deltion and mutant TXNIP in photoreceptors

Depositor

Connie Cepko

Article

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Txnip (Txnip Mouse)

Use

AAV

Mutation

(1-301aa), C247S

Promoter

human red opsin

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1-RUNX3 aa 49-187

Plasmid

#203440

Purpose

Bacterial expression of RUNX5

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

RUNX3 (RUNX3 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-THTAP-SPARTIN, residues 1-101

Plasmid

#180626

Purpose

Bacterial expression for SPARTIN MIT residues 1-101. Has N-terminal HIS-GST tag. Internal ID: WISP20-57.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPARTIN

Tags

HIS-GST

Expression

Bacterial

Mutation

Residues 1-101

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-THTAP-CALPAIN-7 1-165

Plasmid

#180610

Purpose

Bacterial expression vector for CALPAIN-7 residues 1-165. Has N-terminal cleavable HIS-GST tag.Internal ID: WISP21-129

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Tags

HIS-GST

Expression

Bacterial

Mutation

Residues 1-65

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CALPAIN-7 residues 1-165

Plasmid

#180611

Purpose

Bacterial expression vector for CALPAIN-7 residues 1-165, tandem MIT domains. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-25

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-165

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_B-Pho2-1/2-2-tRNA

Plasmid

#161761

Purpose

MODULE B tRNA vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2303 - #91068)

Depositor

Shaun Curtin

Article

Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

Insert

gRNAs

Expression

Bacterial

Promoter

CmYLCV Promoter

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_B-Pho2-1/2-2-Csy4

Plasmid

#161760

Purpose

MODULE B Csy4 vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2103 - #91061)

Depositor

Shaun Curtin

Article

Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

Insert

gRNAs

Expression

Bacterial

Promoter

CmYLCV Promoter

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsTNRC6Biso1_1379-1723del1535-1624_P

Plasmid

#147204

Purpose

Bacterial Expression of HsTNRC6Biso1_1379-1723del1535-1624

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsTNRC6Biso1_1379-1723del1535-1624 (TNRC6B Human)

Expression

Bacterial

Mutation

one non silent mutation T1486A compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsTNRC6Biso1 1-1218_L

Plasmid

#146887

Purpose

Mammalian Expression of HsTNRC6Biso1_1-1218

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsTNRC6Biso1_1-1218 (TNRC6B Human)

Expression

Mammalian

Mutation

4 non silent mutations, S441G, H1088R, Q1106R, Q1…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsTNRC6Biso1_1361-1621-6His_N

Plasmid

#147025

Purpose

Bacterial Expression of HsTNRC6Biso1_1361-1621

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6Biso1_1361-1621 (TNRC6B Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsTNRC6Biso1_1361-1723-6His_L

Plasmid

#146877

Purpose

Bacterial Expression of HsTNRC6Biso1_1361-1723

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6Biso1_1361-1723 (TNRC6B Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Xlrbpa-dsRBD(+6)

Plasmid

#171548

Purpose

Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Xlrbpa (prkra.L Frog)

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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