We narrowed to 12,317 results for: 112

1. LUC2 (L/Sec/deltaP)

   Plasmid

   #112145

   Purpose

   Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

   Depositor

   Paul Copeland

   Article

   Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

   Insert

   Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and deleted AUGA in PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
   

   Expression

   Mammalian

   Mutation

   Cysteine 258 mutated to selenocysteine (U) and d…

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. foxn4

   Plasmid

   #11260

   Depositor

   Lisa Steiner

   Article

   Danilova et al Brain Res Dev Brain Res. 2004 Oct 15. 15

   Insert

   foxn4 (foxn4 Zebrafish)
   

   Expression

   Bacterial

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pCAG-mTHC

   Plasmid

   #112620

   Purpose

   expression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-mTFP1 (H2BC3 Anthozoa, Human)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Tags

   mTeal (mTFP1)

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE80L TriCatcher-RGDSP

   Plasmid

   #112631

   Purpose

   Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcher

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   TriCatcher-RGDSP
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   central RGDSP integrin-binding sequence

   Promoter

   T5

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.hSynap.iGABASnFR.R205A

   Plasmid

   #112162

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Insert

   iGABASnFR.R205A
   

   Use

   AAV

   Mutation

   R205A (no GABA binding)

   Promoter

   hSynapsin

   Available Since

   July 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFPc1 hp47phox (S303/304/328D)

   Plasmid

   #112245

   Purpose

   expression of mutant p47phox

   Depositor

   Simone Di Giovanni

   Article

   Hervera et al Nat Cell Biol. 2018 Mar;20(3):307-319. d

   Insert

   p47phox (NCF1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S303/304/328D

   Promoter

   CMV

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. p415 Glo3

   Plasmid

   #112651

   Purpose

   Expression S.cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Glo3
   

   Expression

   Yeast

   Promoter

   Met25

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-ΔN-RNMT

   Plasmid

   #112708

   Purpose

   Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   RNMT (RNMT Human)
   

   Tags

   FLAG and HIV Rev nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   deleted amino acids 2-120, mutated active-site D2…

   Promoter

   CMV

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA

   Plasmid

   #112921

   Purpose

   Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   EF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-HemmarI2

    Plasmid

    #112815

    Purpose

    gateway cloning destination vector for expressing enhancer driven CD4-IFP2.0-T2A-HO1

    Depositor

    Chun Han

    Article

    Poe et al Genetics. 2018 Nov 30. pii: genetics.118

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. hHpr1-GST

    Plasmid

    #11200

    Depositor

    Robin Reed

    Article

    Masuda et al Genes Dev. 2005 Jul 1. 19(13):1512-7.

    Insert

    Hpr1 (THOC1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Jan. 19, 2006

    Availability

    Academic Institutions and Nonprofits only

12. TCRalpha-L1

    Plasmid

    #11262

    Depositor

    Lisa Steiner

    Article

    Danilova et al Dev Comp Immunol. 2004 Jun . 28(7-8):755

    Insert

    TCR alpha (trac Zebrafish)
    

    Expression

    Bacterial

    Available Since

    March 15, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pESR1-donor

    Plasmid

    #112337

    Purpose

    CRISPR donor plasmid to tag human transcription factor ESR1 with GFP

    Depositor

    Kevin White

    Insert

    ESR1 homology arms flanking EGFP-IRES-Neo cassette (ESR1 Human)
    

    Use

    CRISPR

    Mutation

    Unknown

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. CLTA-mCherry HDRT Source (pTR 177)

    Plasmid

    #112017

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherry

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CLTA-mCherry HDRT (CLTA Human)
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.hSynap.iGABASnFR.F102Y.Y137L

    Plasmid

    #112161

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102Y.Y137L
    

    Use

    AAV

    Mutation

    F102Y.Y137L

    Promoter

    hSynapsin

    Available Since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. rat APOBEC1

    Plasmid

    #112858

    Purpose

    plasmid for expression of rat APOBEC1 in mammalian cells

    Depositor

    Silvestro Conticello

    Article

    Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVHC_PGKneoLox2DTA.2

    Plasmid

    #112622

    Purpose

    H2BVenus_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Venus (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Venus

    Promoter

    No Promoter

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.GFAP.iGABASnFR.F102Y.Y137L

    Plasmid

    #112174

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102Y.Y137L
    

    Use

    AAV

    Mutation

    F102Y.Y137L

    Promoter

    GFAP

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGAT2

    Plasmid

    #112588

    Purpose

    Used for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag-GST fusions

    Depositor

    Marko Hyvönen, Johan Peränen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Tags

    6His-GST

    Expression

    Bacterial

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGAT3

    Plasmid

    #112589

    Purpose

    Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag-GST fusions

    Depositor

    Marko Hyvönen, Johan Peränen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Tags

    6His-GST

    Expression

    Bacterial

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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