We narrowed to 215,080 results for: CIL

1. pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc

   Plasmid

   #129105

   Purpose

   Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone production

   Depositor

   Eleftherios Terry Papoutsakis

   Article

   Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

   Insert

   BsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 11, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pMM7

   Plasmid

   #201213

   Purpose

   Integrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.

   Depositor

   Alex Nielsen

   Article

   Millgaard et al AMB Express. 2023 May 8;13(1):44. doi: 1

   Inserts

   sfGFP
   Spo0A right flank
   Spo0A left flank
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   F11V, T13P, N39D, A179A

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21a(+)_co_L-HicDH

   Plasmid

   #185453

   Purpose

   CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).

   Depositor

   Wolfgang Kroutil

   Article

   Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

   Insert

   L-HicDH from Lactobacillus confusus DSM 20196
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CFFΔC-EGFP

   Plasmid

   #86059

   Purpose

   A mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal) with C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Fibrocystin (Pkhd1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains CD8a luminal and transmembrane domains a…

   Promoter

   CMV

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. CFFΔC-Myc

   Plasmid

   #86060

   Purpose

   A mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal domains) with C-terminus Myc-tag.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Fibrocystin (Pkhd1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Bacterial

   Mutation

   contains CD8a luminal and transmembrane domains a…

   Promoter

   CMV

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015

   Plasmid

   #107582

   Purpose

   B. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

   Depositor

   Paul Freemont

   Article

   Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

   Insert

   GFP+
   

   Use

   Synthetic Biology; E. coli and bacillus shuttle v…

   Tags

   B. megaterium DSM319 xylA leader sequence (MTSSKI…

   Expression

   Bacterial

   Mutation

   F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

   Promoter

   fba promoter Bacillus megaterium DSM319

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEAHISMRSA0482

   Plasmid

   #96968

   Purpose

   expression of methicillin-resistant Staphylococcus aureus orf 0482

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   MRSA ORF0482
   

   Use

   Tags

   N-ter TEV protease cleavable 6His

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. GST-Rab8a-QL

   Plasmid

   #86079

   Purpose

   A bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed glutamine 67 to leucine

   Promoter

   tac

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. GST-Rab8a-TN

   Plasmid

   #86080

   Purpose

   A bacterial expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus GST.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed threonine 22 to aspargine

   Promoter

   tac

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLRC1-NEK10p:NEK10-3XFLAG

    Plasmid

    #137030

    Purpose

    Lentiviral vector for expressing NEK10 under the control of the endogenous NEK10 promoter

    Depositor

    David Sabatini

    Article

    Chivukula et al Nat Med. 2020 Jan 20. pii: 10.1038/s4159

    Insert

    NIMA-like kinase 10 (NEK10 Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Expression

    Mutation

    Promoter

    human NEK10 endogenous promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET22B_PcrA_2Bchimera

    Plasmid

    #102998

    Purpose

    Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA

    Depositor

    Martin Webb

    Insert

    PcrA_2Bchimera
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Bacilllus stearothermophilus PcrA in which the 2B…

    Promoter

    T7

    Available since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK131

    Plasmid

    #71548

    Purpose

    Produces Geobacillus stearothermophilus (Bacillus stearothermophilus) alanine racemase (alr)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    Alanine racemase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTU1-D-spsCD

    Plasmid

    #112788

    Purpose

    Downstream homology arm, SpsC is involved in spore coat polysaccharide synthesis

    Depositor

    Chris French

    Article

    Wicke et al bioRxiv 185108

    Insert

    spsCD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTU1-A-spsCU

    Plasmid

    #112787

    Purpose

    Upstream homology arm, SpsC is involved in spore coat polysaccharide synthesis

    Depositor

    Chris French

    Article

    Wicke et al bioRxiv 185108

    Insert

    spsCU
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTU1-A-rrnOU

    Plasmid

    #112785

    Purpose

    Upstream homology arm, rrnO-23S (23S rRNA)

    Depositor

    Chris French

    Article

    Wicke et al bioRxiv 185108

    Insert

    rrnOU
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTU1-C-cat

    Plasmid

    #112791

    Purpose

    Chloramphenicol resistance cassette (expressing chloramphenicol acetyltransferase)

    Depositor

    Chris French

    Article

    Wicke et al bioRxiv 185108

    Insert

    cat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTU1-D-rrnOD

    Plasmid

    #112786

    Purpose

    Downstream homology arm, rrnO-23S (23S rRNA)

    Depositor

    Chris French

    Article

    Wicke et al bioRxiv 185108

    Insert

    rrnOD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTU1-D-amyED

    Plasmid

    #112782

    Purpose

    Downstream homology arm, amyE encodes _-amylase

    Depositor

    Chris French

    Article

    Wicke et al bioRxiv 185108

    Insert

    amyED
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTU1-A-amyEU

    Plasmid

    #112781

    Purpose

    Upstream homology arm, amyE encodes _-amylase

    Depositor

    Chris French

    Article

    Wicke et al bioRxiv 185108

    Insert

    amyEU
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTU1-A-pdh_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid

    #107576

    Purpose

    B. megaterium DSM319 pdh promoter, GFP+ with malachite green mRNA aptamer in Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

    Depositor

    Paul Freemont

    Article

    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Insert

    GFP+
    

    Use

    Synthetic Biology

    Tags

    B. megaterium DSM319 xylA leader sequence (MTSSKI…

    Expression

    Bacterial

    Mutation

    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

    Promoter

    pdh promoter Bacillus megaterium DSM319

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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