We narrowed to 26,519 results for: igh

1. pHH0103_PLK1_POLO-box

   Plasmid

   #109812

   Purpose

   Protein expression and purification of PLK1_POLO-box

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PLK1_POLO-box (PLK1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDGE669

   Plasmid

   #153235

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDGE672

   Plasmid

   #153238

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   GFP

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDGE473

   Plasmid

   #153264

   Purpose

   M1E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDGE500

   Plasmid

   #153273

   Purpose

   M6E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDGE502

   Plasmid

   #153275

   Purpose

   M8E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS

   Plasmid

   #153443

   Purpose

   E. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc.XDocB
   

   Use

   Tags

   6xHis and ybbr tag

   Expression

   Bacterial

   Mutation

   Serine 199 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC19-hsg(G:H)

   Plasmid

   #138259

   Purpose

   Expression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter system

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   mCherry targeting sgRNAs G and H
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCB6-Occludin

   Plasmid

   #136637

   Purpose

   Expression of Occludin in mammalian cells

   Depositor

   Maria Balda, Karl Matter

   Article

   Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

   Insert

   Occludin (OCLN Chicken)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCB6-HAOccludin

    Plasmid

    #136638

    Purpose

    Expression of HAOccludin in mammalian cells

    Depositor

    Maria Balda, Karl Matter

    Article

    Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

    Insert

    HAOccludin (OCLN Chicken)
    

    Use

    Tags

    HA (MQDLPGNDNSTAG)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCB6-OccludinCT3

    Plasmid

    #136639

    Purpose

    Expression of OccludinCT3 in mammalian cells

    Depositor

    Maria Balda, Karl Matter

    Article

    Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

    Insert

    OccludinCT3 (OCLN Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal cytoplasid domain

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCB6-HAOccludinCT3

    Plasmid

    #136640

    Purpose

    Expression of HAOccludinCT3 in mammalian cells

    Depositor

    Maria Balda, Karl Matter

    Article

    Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

    Insert

    HAOccludinCT3 (OCLN Chicken)
    

    Use

    Tags

    HA (MQDLPGNDNSTAG)

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal cytoplasid domain

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEF1a-T2-GpNLuc

    Plasmid

    #135949

    Purpose

    Expresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Use

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEF1a-T2-OgNLuc

    Plasmid

    #135951

    Purpose

    Expresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    OgNLuc LumiFluor
    

    Use

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pY71-tet.I.-1

    Plasmid

    #133539

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 36 bp upstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pY71-tet.I.25

    Plasmid

    #133538

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 28 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pY71-tet.I.20

    Plasmid

    #133537

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 23 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pY71-tet.I.14

    Plasmid

    #133535

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 17 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pY71-tet.I.13

    Plasmid

    #133534

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 16 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pY71-tet.I.12

    Plasmid

    #133533

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 15 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pY71-tet.I.11

    Plasmid

    #133532

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 14 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pY71-tet.I.10

    Plasmid

    #133531

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 13 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pY71-tet.I.9

    Plasmid

    #133530

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 12 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pY71-tet.I.7

    Plasmid

    #133529

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 10 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pY71-tet.I.6

    Plasmid

    #133528

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 9 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pY71-tet.I.5

    Plasmid

    #133527

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 8 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pY71-tet.I.3

    Plasmid

    #133525

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 6 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pY71-tet.I.2

    Plasmid

    #133524

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 5 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pY71-tet.I.4

    Plasmid

    #133526

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 7 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pY71-tet.I.1

    Plasmid

    #133523

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 4 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pOVC1_X_AsPT1-LOV2-EcSsra

    Plasmid

    #131217

    Purpose

    Expresses AsPT1-LOV2-EcSsra (Avena Sativa) preceded by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    AsPT1-LOV2-EcSsra
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pOVC1_X_AsPT1-LOV2-pep

    Plasmid

    #131215

    Purpose

    Expresses AsPT1-LOV2-pep (Avena sativa) preceded by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    AsPT1-LOV2-pep
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pYPQ150-x3.7

    Plasmid

    #124308

    Purpose

    Gateway entry vector with pcoCas9-x3.7 (Plant codon optimized)

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

    Insert

    pcoCas9-x3.7
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pYPQ151D10A-x3.7

    Plasmid

    #124309

    Purpose

    Gateway entry vector with pcoCas9D10A-x3.7 (Plant codon optimized)

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

    Insert

    pcoCas9D10A-x3.7
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJL1-neoR

    Plasmid

    #117048

    Purpose

    In vitro expression of neoR (kanamycin resistance gene) from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    neoR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET41a iGluSnFR E25R

    Plasmid

    #119831

    Purpose

    Production of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR E25R
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E25R

    Promoter

    T7

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET27b-ecDHFR-N23PP

    Plasmid

    #110828

    Purpose

    Bacterial expression of E.coli DHFR N23PP mutant

    Depositor

    Stephen Benkovic

    Article

    Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N23 mutated to P23; a proline residue inserted be…

    Promoter

    T7

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGS_C_3xFLAG

    Plasmid

    #101653

    Purpose

    Expression of type I signal-peptide containing proteins with C-terminal 3xFLAG-tag in insect cells

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    LRP6 Signal peptide, MCS linker, 3xFLAG tag
    

    Use

    Tags

    3xFLAG tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMflT-o2

    Plasmid

    #101310

    Purpose

    Contains: oriC2 fragment of M. florum L1 replication origin (dnaA/dnaN intergenic region), colE1 replication origin, recoded tetM resistance gene.

    Depositor

    Sébastien Rodrigue

    Article

    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

    Insert

    oriC2 of M. florum strain L1 (dnaA/dnaN intergenic region)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMflT-o1

    Plasmid

    #101309

    Purpose

    Contains: oriC1 fragment of M. florum L1 replication origin (rpmH/dnaA intergenic region), colE1 replication origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.

    Depositor

    Sébastien Rodrigue

    Article

    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

    Insert

    oriC1 of M. florum strain L1 (rpmH/dnaA intergenic region)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge