163,297 results

1. GST PTBP1 isoform 4

   Plasmid

   #108594

   Purpose

   Recombinant GST tagged protein production

   Depositor

   Chris Smith

   Article

   Coelho et al EMBO J. 2015 Mar 4;34(5):653-68. doi: 10

   Insert

   PTBP1 isoform 4 (PTBP1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   isoform 4 (isoform a)

   Promoter

   tac

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRIPZ shIFNAR

   Plasmid

   #127699

   Purpose

   Doxycyclin inducible shRNA knockdown of mouse IFNAR gene

   Depositor

   Sandra Demaria

   Article

   Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

   Insert

   shIFNAR1 Mmu (Ifnar1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJCSUP35

   Plasmid

   #1088

   Depositor

   Susan Lindquist

   Article

   Glover et al Cell 1997 May 30;89(5):811-9.

   Insert

   SUP35 (SUP35 Budding Yeast)
   

   Use

   Tags

   HIS tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 26, 2005

   Availability

   Academic Institutions and Nonprofits only

4. PARP9-c020

   Plasmid

   #173097

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP9 (PARP9 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   N310-N493

   Promoter

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT3-EF1aH N90-beta-catenin

   Plasmid

   #86499

   Purpose

   Mammalian expression of Myc tagged N90 beta-catenin

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 1-90; constitutively acti…

   Promoter

   EF1a

   Available since

   Feb. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDL-hu FceRI alpha

   Plasmid

   #8365

   Depositor

   Jean Kinet

   Article

   Kochan et al Nucleic Acids Res 1988 Apr 25;16(8):3584

   Insert

   human Fc epsilon RI alpha cDNA (FCER1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA-HDAC6.DC-FLAG

   Plasmid

   #30483

   Depositor

   Tso-Pang Yao

   Article

   Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

   Insert

   HDAC6 (HDAC6 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Catalytically inactive mutant (H216/611A)

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMK321 (TOP2A-mAC (Neo))

   Plasmid

   #140652

   Purpose

   TOP2A tagging with mAID-Clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   TOP2A (TOP2A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-Rab27B N133I

   Plasmid

   #89449

   Purpose

   Expression of GFP-tagged human Rab27B N133I in mammalian cells

   Depositor

   Wendy Westbroek

   Article

   Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

   Insert

   human Rab27B N133I (RAB27B Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   N133I

   Promoter

   CMV

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNAI-GAL4-CREB

    Plasmid

    #46769

    Purpose

    Expression of GAL4 fusion to wild type CREB

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    GAL4(1-147)-CREB (Creb1, GAL4 Rat, Budding Yeast)
    

    Use

    Tags

    GAL4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

    Plasmid

    #138143

    Purpose

    Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PguCas13b
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSynapsin1-FLEx-axon-GCaMP6s (AAV9)

    Viral Prep

    #112010-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSynapsin1-FLEx-axon-GCaMP6s (#112010). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-FLEx-axon-GCaMP6s plasmid DNA. Synapsin-driven, cre-dependent axon-targeted GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Promoter

    Syn

    Tags

    None

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

13. T7-VEE-IRES-Puro

    Plasmid

    #58970

    Purpose

    noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Type

    Empty backbone

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    26S subgenomic promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. LV anti-FITC-LaG16-NRR-T2A-BFP

    Plasmid

    #194929

    Purpose

    LV construct encoding NRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-NRR-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mARGAna (cassette 1, transient)

    Plasmid

    #197588

    Purpose

    Second-generation mammalian acoustic reporter gene derived from Anabaena

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    mARGAna (cassette 1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. mARGAna (cassette 2, transient)

    Plasmid

    #197589

    Purpose

    Second-generation mammalian acoustic reporter gene derived from Anabaena

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    mARGAna (cassette 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-Ostir-puro

    Plasmid

    #161972

    Purpose

    Expresses Ostir and puromycin resistance gene in mammalian cells

    Depositor

    Joanna Wysocka

    Article

    Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

    Insert

    Ostir-3xHA-p2a-puroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDZ513 (pHIS MET25pro PP7-PS-yeGFP)

    Plasmid

    #45931

    Depositor

    Daniel Zenklusen

    Article

    Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

    Insert

    PCP-PS
    

    Use

    Tags

    2x-yeGFP

    Expression

    Yeast

    Mutation

    Promoter

    Met25

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-Rab27B T23N

    Plasmid

    #89450

    Purpose

    Expression of GFP-tagged human Rab27B T23N in mammalian cells

    Depositor

    Wendy Westbroek

    Article

    Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

    Insert

    human Rab27B T23N (RAB27B Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    T23N

    Promoter

    CMV

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CaMKIIa-oChIEF(E163A/T199C)-P2A-EGFP

    Plasmid

    #51093

    Purpose

    Forebrain principal neuron expression of oChIEF kinetic variant E163A/T199C with physically uncoupled EGFP fluorophore

    Depositor

    Jonathan Ting

    Insert

    oChIEF(E163A/T199C)
    

    Use

    AAV

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    E163A/T199C

    Promoter

    CaMKIIa

    Available since

    May 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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