We narrowed to 6,113 results for: arl

1. SpyTag003(M115A)-Talin rod-mCherry

   Plasmid

   #210025

   Purpose

   Expression of SpyTag003(M115A)-Talin rod(434-2541)-mCherry in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

   Insert

   SpyTag003(M115A)-Talin1 rod domain(434-2541)-mCherry
   

   Expression

   Mammalian

   Mutation

   SpyTag003 M115A mutation to modify SpyCatcher003 …

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyTag003(V114T, V116T)-Talin rod-mCherry

   Plasmid

   #210027

   Purpose

   Expression of SpyTag003(V114T, V116T)-Talin rod(434-2541)-mCherry in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

   Insert

   SpyTag003(V114T,V116T)-Talin1 rod domain(434-2541)-mCherry
   

   Expression

   Mammalian

   Mutation

   SpyTag003 V114T and V116T mutations to modify Spy…

   Promoter

   CMV

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-Talin head-SpyCatcher003(K31TAG)

   Plasmid

   #210023

   Purpose

   Expression of EGFP-Talin head(1-433)-SpyCatcher003(K31TAG) in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

   Insert

   EGFP-Talin head (1-433)-SpyCatcher003(K31TAG)
   

   Expression

   Mammalian

   Mutation

   SpyCatcher003 with K31TAG (Amber stop codon) muta…

   Promoter

   CMV

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXPR_068

   Plasmid

   #164555

   Purpose

   Lentiviral vector that enables Flp-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

   Depositor

   Arlene Sharpe

   Article

   LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

   Insert

   sgRNA cassette
   

   Use

   CRISPR and Lentiviral; Flp/frt

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET19-TdTc253

   Plasmid

   #207464

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 253

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   MTdTc253 (Dntt Mouse)
   

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Ser253Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET19-TdTc188

   Plasmid

   #207463

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 188

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   TdTc188 (Dntt Mouse)
   

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Cys216Ser, Cys302Ala, Cys378Ala, and Cys438Ser

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET19-TdTc180

   Plasmid

   #207462

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 180

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   TdTc180 (Dntt Mouse)
   

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Glu180Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET19-TdTc302

   Plasmid

   #207461

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   TdTc302 (Dntt Mouse)
   

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXPR_023d-sgCiPOLR2D

   Plasmid

   #202445

   Purpose

   Expression of a CRISPRi guide targeting POLR2D

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   POLR2D gRNA (POLR2D Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXPR_003-sgUBR4

    Plasmid

    #202442

    Purpose

    Expression of a guide RNA targeting UBR4

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    UBR4 gRNA (UBR4 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXPR_003-sgUBA6

    Plasmid

    #202440

    Purpose

    Expression of a guide RNA targeting UBA6

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    UBA6 gRNA (UBA6 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSN554

    Plasmid

    #177361

    Purpose

    Expresses human KIF1A(1-393) fused with leucine zipper, mScarlet and StrepII tag

    Depositor

    Shinsuke Niwa

    Article

    Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

    Insert

    KIF1A (KIF1A Human)
    

    Tags

    Leucine zipper, StrepII tag, and mScarlet

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. LB001: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#2 gRNA

    Plasmid

    #131757

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#2 Sp/xCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Promoter

    hU6

    Available Since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. LA901: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#1 gRNA

    Plasmid

    #131756

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#1 Sp/xCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Promoter

    hU6

    Available Since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. JV201: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#1 gRNA

    Plasmid

    #131755

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#1 SaCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Promoter

    hU6

    Available Since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LB101: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#2 gRNA

    Plasmid

    #131758

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#2 SaCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Promoter

    hU6

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)

    Plasmid

    #123340

    Purpose

    Negative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-EKAR-EV(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Venus-cpVenus-FLARE-CKAR(T/A)

    Plasmid

    #123344

    Purpose

    Negative control mutant for Venus-cpVenus-FLARE-CKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-CKAR(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Cerulean3-cpCerulean3-FLARE-AKAR

    Plasmid

    #123336

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-cpCerulean3-FLARE-AKAR
    

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKI-cjACTB-EGFP-3'short

    Plasmid

    #129749

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    ACTB (ACTB C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    EGFP

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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