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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,812 results for: cloning

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Showing: 3841 - 3860 of 63812 results

  2. pSLQ10875

    Plasmid
    #183963
    Purpose
    CBA-hyperdCas12a-HA-KRAB-mCherry
    Depositor
    Stanley Qi
    Publication
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, KRAB, and mCherry
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CBA
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183963-plasmid-map-sequence-id-363118

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  5. pH6-MBP-TEV-BirA

    Plasmid
    #179694
    Purpose
    Expresses His6 tagged MBP-BirA fusion protein
    Depositor
    Mark Arbing
    Insert
    BirA
    Tags
    His6-MBP
    Expression
    Bacterial
    Available since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    179694-plasmid-map-sequence-id-352195

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  8. pRecT_2

    Plasmid
    #167546
    Purpose
    Encodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.
    Depositor
    Howard Hang
    Publication
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Insert
    E. faecium Com15 recT under an IPTG inducible promoter, with lacI
    Expression
    Bacterial
    Promoter
    IPTG
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    167546-plasmid-map-sequence-id-332204

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  11. pM1s3AsG

    Plasmid
    #137921
    Purpose
    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expression
    Depositor
    Neel Joshi
    Publication
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Insert
    Superfolder GFP
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Expression
    Bacterial
    Promoter
    J23101
    Available since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    137921-plasmid-map-sequence-id-322876

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  13. C1-MPAct-mCherry

    Plasmid
    #155222
    Purpose
    Monitor changes in the density of membrane-proximal F-actin (MPA)
    Depositor
    Tobias Meyer
    Publication
    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
    Tags
    mCherry
    Expression
    Mammalian
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155222-plasmid-map-sequence-id-310131

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  14. pLEX307-APN-puro

    Plasmid
    #158454
    Purpose
    Constitutive lentiviral expression of human APN.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    APN (ANPEP Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158454-plasmid-map-sequence-id-308145

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  16. pCAGGs-Venus

    Plasmid
    #127346
    Purpose
    Expresses Venus constitutively via the CAGGS promoter in mammalian cells
    Depositor
    Phil Sharp
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus Fluorescent Protein
    Expression
    Mammalian
    Promoter
    CAGGS Promoter
    Available since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127346-plasmid-map-sequence-id-246571

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Showing: 3841 - 3860 of 63812 results