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Str-KDEL_flGalT-SBP-tagBFP

Plasmid

#65274

Purpose

synchronize trafficking of GalT from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pWKS1583

Plasmid

#70188

Purpose

Modular vector for secreted amylase reporter expression

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

Insert

amyEopt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pFU-no toxin-PE

Plasmid

#24149

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

Insert

PDGF-receptor-transmembrane domain (PDGFRB Aequorea victoria, Human)

Use

Lentiviral

Tags

EGFP and FLAG

Expression

Mammalian

Available Since

April 8, 2010

Availability

Academic Institutions and Nonprofits only
ManII-SBP-EGFP_puromycin

Plasmid

#65260

Purpose

ManII reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated Mannosidae II

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pKMV-VioE

Plasmid

#65351

Purpose

systhesis of vioE gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioE

Use

Synthetic Biology

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM159

Plasmid

#84084

Purpose

target miRNA159 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM159

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pDS272

Plasmid

#34975

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2-GFP-LOVpep (MID2 A. sativa, Budding Yeast)

Expression

Yeast

Mutation

T406A, T407A

Promoter

TEF

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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mOrange-H2B-6

Plasmid

#57942

Purpose

Localization: Nucleus/Histones, Excitation: 548, Emission: 562

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Insert

H2B (H2BC11 Human)

Tags

mOrange

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pCFJ1202

Plasmid

#44484

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, peel-1, MCS

Use

Multiple cloning site vector

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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Syt1LeftArm-3XGS-GCaMP6s-SV40-3XP3-dsRed-SV40-Syt1RightArm

Plasmid

#159636

Purpose

Donor plasmid for generating Syt1:GCaMP6s strain in Aedes aegypti with CRISPR/Cas9

Depositor

Carolyn McBride

Article

Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

Insert

GCaMP6s

Expression

Insect

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP

Plasmid

#31478

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV HA NLS Y2 K227M T2A BFP

Use

Lentiviral

Tags

HA and NLS

Mutation

F13Y, S111Y, K227M = Y2 nickase "reo" …

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pFGC5941DR5-PacI-ath-STTM390

Plasmid

#84117

Purpose

target miRNA390 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM390

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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TN-XXL pRSETB

Plasmid

#45796

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

Insert

TN-XXL

Tags

6xHIS, ECFP, and YFP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only
pKMV-VioA

Plasmid

#65347

Purpose

systhesis of vioA gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioA

Use

Synthetic Biology

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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Clover-Lysosomes-20

Plasmid

#56528

Purpose

Localization: Lysosome Membrane, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

LAMP1

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pCaSper4 cyto roGFP2-Orp1

Plasmid

#64998

Purpose

Expression of cytosolic roGFP2-Orp1 under the alphaTub84B promoter

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Tags

roGFP2

Expression

Insect

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM858a/b

Plasmid

#84177

Purpose

target miRNA858 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM858a/b

Expression

Plant

Promoter

2x35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-185-5p.sensor.PGK.dNGFR.WPRE

Plasmid

#85930

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-185-5p Sensor (MIR185 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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ciCas9(L22)_pcDNA5

Plasmid

#100551

Purpose

Expresses ciCas9(L22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9(L22)

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRbm10/Cre

Plasmid

#89648

Purpose

Expresses an Rbm10-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Rbm10 (Rbm10 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-Zdk1-VAV2 DH/PH/C1

Plasmid

#81060

Purpose

mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
VAV2 DH/PH/C1 (VAV2 Human)

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pRNA-U6-miR122 sponge

Plasmid

#35665

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

U6 miR-122sponge

Promoter

U6

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_TOP1

Plasmid

#68423

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ1001

Plasmid

#44476

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, unc-119, peel-1, [4-3] Gateway

Use

Gateway destination vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM828

Plasmid

#84157

Purpose

target miRNA828 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM828

Expression

Plant

Promoter

2x35S

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only
Lenti-sgSmad4/Cre

Plasmid

#89651

Purpose

Expresses a Smad4-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Smad4 (Smad4 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ496

Plasmid

#44488

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

500bp Mos1, unc-119, fosmid cassette

Use

Fosmid recombineering cassette

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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ManII-SBP-mCherry_puromycin

Plasmid

#65261

Purpose

ManII reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated Mannosidae II

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDS271

Plasmid

#34974

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2-GFP-LOVpep (MID2 A. sativa, Budding Yeast)

Expression

Yeast

Mutation

T406A, T407A, V416I

Promoter

TEF

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-21-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85857

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-21-3p Sensor (MIR21 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRNA-U6-TuDmiR122

Plasmid

#35669

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

TuD miR-122

Promoter

U6

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ1002

Plasmid

#44477

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, peel-1, [4-3] Gateway

Use

Gateway destination vector

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
ciCas9(F22)_pcDNA5

Plasmid

#100552

Purpose

Expresses ciCas9(F22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9(F22)

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-C-Src-7

Plasmid

#54052

Purpose

Localization: Membrane/Vesicles, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

C-Src

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pFGC5941-PacI-ath-STTM843

Plasmid

#84169

Purpose

target miRNA843 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM843

Expression

Plant

Promoter

2x35S

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM845

Plasmid

#84170

Purpose

target miRNA845 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM845

Expression

Plant

Promoter

2x35S

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p416GAL1-Zipp.-L-IFP-F[1] (Yeast)

Plasmid

#52895

Purpose

Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Zipp.-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pAAVsc CB6PI Gpluc7XmiR-122T

Plasmid

#35647

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

7 bulged miR-122 target sites

Use

AAV

Promoter

CB

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pReporter_13_switchBP

Plasmid

#62848

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). attP upstream of attB in this plasmid.

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_13_switchBP

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM173

Plasmid

#84101

Purpose

target miRNA173 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM173

Expression

Plant

Promoter

2x35S

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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