We narrowed to 16,562 results for: IGH@

1. pLTR PKC epsilon

   Plasmid

   #8420

   Depositor

   Frederic Mushinski

   Article

   Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

   Insert

   Mouse Protein Kinase C epsilon (Prkce Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 5, 2007

   Availability

   Academic Institutions and Nonprofits only

2. TAL2206

   Plasmid

   #36669

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   FLCN-TALEN_Left (Flcn Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

3. TAL2270

   Plasmid

   #36707

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   GLI1-TALEN_Left (GLI1 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

4. Dom34(265-386)

   Plasmid

   #19102

   Depositor

   SeWon Suh

   Article

   Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

   Insert

   Protein DOM34 (DOM34 Budding Yeast)
   

   Use

   Tags

   C tag and N tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pmalc4x_V426F_TEV

   Plasmid

   #47702

   Purpose

   Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation V426F

   Depositor

   Raquel Lieberman

   Article

   Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

   Insert

   Olfactomedin-like domain with V426F mutation (MYOC Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed V426F

   Promoter

   Ptac

   Available since

   Sept. 9, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. PBB-79

   Plasmid

   #8813

   Depositor

   Cynthia Wolberger

   Article

   Batchelor et al Science 1998 Feb 13;279(5353):1037-41.

   Insert

   GABPb1 (1-157) (Gabpb1 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

7. PA-mCherry-miniSOG-Cx26-7

   Plasmid

   #57786

   Purpose

   Localization: Gap Junctions, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   Cx26
   

   Use

   Tags

   PA-mCherry-miniSOG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

8. miniSOG-Zyxin-6

   Plasmid

   #57781

   Purpose

   Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   Zyxin (ZYX Human)
   

   Use

   Tags

   miniSOG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. PA-mCherry-miniSOG-Talin-N-10

   Plasmid

   #57797

   Purpose

   Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   Talin
   

   Use

   Tags

   PA-mCherry-miniSOG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

10. TALEN-CCR5R18-NtermN1

    Plasmid

    #51445

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with N1 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) N1 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Mutation

    K150Q

    Promoter

    CMV

    Available since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHL718

    Plasmid

    #62826

    Purpose

    Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::mCherry::T1 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Inserts

    gfp
    mCherry
    

    Use

    Synthetic Biology

    Tags

    ipex leader and ompC leader

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1 and pLtetO-1

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

12. mCerulean3-Dectin1A-C-10

    Plasmid

    #55413

    Purpose

    Localization: Membrane, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Dectin1A (CLEC7A Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

13. tdminiSOG-PCNA-19

    Plasmid

    #57806

    Purpose

    Localization: Nucleus, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    tdminiSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pMTH PKC gamma

    Plasmid

    #8412

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

    Insert

    Protein Kinase C gamma (Prkcg Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

15. Dom34(265-377)

    Plasmid

    #19103

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    C tag and N tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

16. TALEN-CCR5R18-NtermN2

    Plasmid

    #51447

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with N2 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) N2 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Mutation

    K150Q and K153Q

    Promoter

    CMV

    Available since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLTR PKC gamma

    Plasmid

    #8418

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

    Insert

    Protein Kinase C gamma (Prkcg Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pGST1-GGA2-VHS

    Plasmid

    #44419

    Depositor

    James Hurley

    Article

    Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

    Insert

    GGA2-VHS (GGA2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Alanine 23 changed to Threonine

    Promoter

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pRCIscript-crhr2_TAL1A

    Plasmid

    #44236

    Depositor

    Stephen Ekker, Karl Clark

    Article

    Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

    Insert

    crhr2 pTAL scaffold 1A
    

    Use

    Mrna transcription vector

    Tags

    Expression

    Mutation

    Promoter

    T3

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRCIscript-crhr2_TAL1B

    Plasmid

    #44237

    Depositor

    Stephen Ekker, Karl Clark

    Article

    Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

    Insert

    crhr2 pTAL scaffold 1B
    

    Use

    Mrna transcription vector

    Tags

    Expression

    Mutation

    Promoter

    T3

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GoldyTALEN-cdh5 TAL1

    Plasmid

    #41833

    Depositor

    Jeffrey Essner

    Article

    Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

    Insert

    cadherin 5 (cdh5 Zebrafish)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TAL2324

    Plasmid

    #36750

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    MAP2K4-TALEN_Left (Map2k4 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

23. TAL2448

    Plasmid

    #36837

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    PDGFRB-TALEN_Left (Pdgfrb Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

24. mBaoJin-lifeactin

    Plasmid

    #229608

    Purpose

    Expresses mBaoJin-lifeactin in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

    Insert

    mBaoJin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. rsZACRO/pcDNA3

    Plasmid

    #236199

    Purpose

    Mammalian expression of rsZACRO, a positively reversibly photoswitchable fluorescent protein

    Depositor

    Takeharu Nagai

    Article

    Ozaki-Noma et al ACS Nano. 2025 Feb 25;19(7):7188-7201. d

    Insert

    rsZACRO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. rsZACRO/pRSETB

    Plasmid

    #236200

    Purpose

    Bacterial expression of rsZACRO, a positively reversibly photoswitchable fluorescent protein

    Depositor

    Takeharu Nagai

    Article

    Ozaki-Noma et al ACS Nano. 2025 Feb 25;19(7):7188-7201. d

    Insert

    rsZACRO
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBOB-3xFlag-mGpr75

    Plasmid

    #236744

    Purpose

    Express 3xFlag N-terminal tagged wild-type mouse GPR75 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

    Insert

    Gpr75 (Gpr75 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #236406

    Purpose

    Expresses cytosolic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PIV3HN-05 LC

    Plasmid

    #236190

    Purpose

    Produces the light chain of the human monoclonal antibody PIV3HN-05

    Depositor

    Jarrod Mousa

    Article

    Miller et al Nat Commun. 2024 Dec 30;15(1):10825. doi

    Insert

    PIV3HN-05 light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAPH07

    Plasmid

    #234345

    Purpose

    Library-scale IPTG-inducible gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpK

    Depositor

    Jerome Fox

    Article

    Hren et al Proc Natl Acad Sci U S A. 2025 Mar 25;12

    Insert

    Type II dCas9 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAPH021

    Plasmid

    #234346

    Purpose

    Library-scale IPTG-inducible single transcript dual-gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpK

    Depositor

    Jerome Fox

    Article

    Hren et al Proc Natl Acad Sci U S A. 2025 Mar 25;12

    Inserts

    Type II dCas9 sgRNA
    Type II dCas9 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. cACTB

    Plasmid

    #236544

    Purpose

    For expression of ACTB in mammalian cells

    Depositor

    Sandra Citi

    Article

    Mauperin et al Nat Commun. 2025 Mar 13;16(1):2514. doi:

    Insert

    ACTB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    hpACTB

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. EW408 (ZNF35tar-compact)x8-H2B-GFP

    Plasmid

    #236129

    Purpose

    Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with eight ZNF35 binding sites upstream

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    E1b minimal promoter with eight ZNF35 binding sit…

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHD-FlpTag-DsRed-HDR-ph2

    Plasmid

    #231902

    Purpose

    dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 2.

    Depositor

    Alexander Borst

    Article

    Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

    Insert

    Frt-Frt14-SD-GFP-SA-Frt-Frt14
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHD-FlpTag-DsRed-HDR-ph1

    Plasmid

    #231901

    Purpose

    dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 1.

    Depositor

    Alexander Borst

    Article

    Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

    Insert

    Frt-Frt14-SD-GFP-SA-Frt-Frt14
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHD-FlpTag-DsRed-HDR-ph0

    Plasmid

    #231900

    Purpose

    dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 0.

    Depositor

    Alexander Borst

    Article

    Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

    Insert

    Frt-Frt14-SD-GFP-SA-Frt-Frt14
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLenti_PEmax-2A-BSD

    Plasmid

    #234071

    Purpose

    For lentiviral expression of PEmax with blasticidin resistance

    Depositor

    Greg Findlay

    Article

    Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

    Insert

    PEmax
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α core promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pIVT-Bxb1-I87L, A369P, E434G

    Plasmid

    #232750

    Purpose

    IVT template for making modRNA encoding mutant Bxb1modRNA (I87L, A369P, E434G)

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    Bxb1
    

    Use

    In vitro transcription

    Tags

    HA and SV40 NLS

    Expression

    Mutation

    I87L, A369P, E434G

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pXJ-Myc-KLF16

    Plasmid

    #232655

    Purpose

    transient expresses KLF16 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    KLF16 (KLF16 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Bxb1-GT_CAG-ITPKA-mScarlet-I

    Plasmid

    #229778

    Purpose

    Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mScarlet-I
    

    Use

    Synthetic Biology

    Tags

    ITPKA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCMV-YR-ICUE-mVenus

    Plasmid

    #231787

    Purpose

    Visualization of cAMP dynamics using YR-ICUE-mVenus

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    YR-ICUE-mVenus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCMV-YR-ICUE-mGold

    Plasmid

    #231788

    Purpose

    Visualization of cAMP dynamics using YR-ICUE-mGold

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    YR-ICUE-mGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pRExT2A-NT-BTy

    Plasmid

    #232206

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    3xTy
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pRExT2A-CT-PTy

    Plasmid

    #232203

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: 3xTy-T2A-PAC-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    3xTy
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pRExT2A-CT-BTy

    Plasmid

    #232209

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: 3xTy-T2A-BSD-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    3xTy
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pRExT2A-NT-PTy

    Plasmid

    #232202

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    3xTy
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. Bxb1-GA_AIO-TetOn_mScarlet_Convergent

    Plasmid

    #229790

    Purpose

    Bxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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48. Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent

    Plasmid

    #229800

    Purpose

    Bxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1a

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mGreenLantern; mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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49. pRSFDUET-1_VNp-anti-Lysozyme fAb

    Plasmid

    #214951

    Purpose

    Bacterial expression of anti-chicken lysozyme (folded) Fab light and heavy chain VNp fusions

    Depositor

    Dan Mulvihill

    Article

    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

    Inserts

    VNp-anti-Lysozyme Fab heavy chain fusion
    VNp-anti-Lysozyme Fab light chain fusion
    

    Use

    Tags

    VNp

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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50. E547* FL USP7

    Plasmid

    #229001

    Purpose

    E547-mutant of human USP7

    Depositor

    Irina Bezsonova

    Insert

    USP7 1-546 (USP7 Human)
    

    Use

    Tags

    6xHis cleavable with thrombin

    Expression

    Bacterial

    Mutation

    E547 mutated to stop codon

    Promoter

    T7

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge