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Plasmid#123475PurposeMoChlo kitDepositorInsertPclpP (PEP)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_ALPPL2 Hinge Notch SNIPR
Plasmid#188377PurposeLentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA-PHLPP1 full length
Plasmid#37100PurposeExpresses HA tagged, full-length human PHLPP1 in mammalian cellsDepositorInsertPHLPP1 (PHLPP1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-PHLPP1 N-terminal truncation
Plasmid#22404PurposeExpresses a HA tagged, N-terminal truncation of human PHLPP1 in mammalian cellsDepositorInsertPHLPP1 (PHLPP1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBEST_OR2-OR1-Pr-UTR1_ClpP-T500
Plasmid#153302PurposeExpresses ClpP protease in the cell-free TX-TL systemDepositorInsertclpP (clpP Escherichia coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK-Zm PclpP::Gg10 leader
Plasmid#123512PurposeMoChlo kitDepositorInsertZm PclpP::Gg10 leader
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA-PHLPP1 full length
Plasmid#37100PurposeExpresses HA tagged, full-length human PHLPP1 in mammalian cellsDepositorInsertPHLPP1 (PHLPP1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-PHLPP1 N-terminal truncation
Plasmid#22404PurposeExpresses a HA tagged, N-terminal truncation of human PHLPP1 in mammalian cellsDepositorInsertPHLPP1 (PHLPP1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
araBAD-BCD20-ClpP-BCD17-ClpX
Plasmid#153301PurposeExpresses clpX and clpP in E.ColiDepositorUseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaPH
Plasmid#22930PurposeMammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PH domainDepositorInsertPHLPP1-deltaPH (PHLPP1 Human)
UseTagsHAExpressionMammalianMutationIncludes 127-1205 aa. Deleted amino-terminal PH d…PromoterCMVAvailable sinceFeb. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC
Plasmid#22931PurposeMammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PDZ binding motif (delta C)DepositorInsertPHLPP1-deltaC (PHLPP1 Human)
UseTagsHAExpressionMammalianMutationPHLPP-deltaC, deletion of PDZ binding domain--the…PromoterCMVAvailable sinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaPH
Plasmid#22930PurposeMammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PH domainDepositorInsertPHLPP1-deltaPH (PHLPP1 Human)
UseTagsHAExpressionMammalianMutationIncludes 127-1205 aa. Deleted amino-terminal PH d…PromoterCMVAvailable sinceFeb. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC
Plasmid#22931PurposeMammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PDZ binding motif (delta C)DepositorInsertPHLPP1-deltaC (PHLPP1 Human)
UseTagsHAExpressionMammalianMutationPHLPP-deltaC, deletion of PDZ binding domain--the…PromoterCMVAvailable sinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_Hinge Notch SNIPR (HNF1A, ALPPL2 scFv)
Plasmid#188385PurposeLentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factorDepositorInsertPGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr
Plasmid#218766Purposeencodes EcTyrRS-VSMA* and EcTyr-tRNA-TAGDepositorInsertEcTyrRS-VSMA*
UseTagsExpressionBacterialMutationY37V, D167G, D182S, F183M, L186A, D265RPromotertacIAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr
Plasmid#218767Purposeencodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAGDepositorInsertEcTyrRS-pAAFRS-9
UseTagsExpressionBacterialMutationY37G, L71V, D182C, F183Y, L186C, D265RPromotertacIAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAJE3-E7
Plasmid#214359PurposeExpresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteinsDepositorInserts2nd generation Tet2 "E7" aminoacyl tRNA synthetase
Lpp promoted M. jannaschii tRNA
Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
Orthogonal "D4II" ColE1 origin
UseTagsExpressionBacterialMutationPromoterAmpR and lppAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKB224
Plasmid#170014PurposeEncodes LacI-controlled expression of surface-displayed NC and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
NC
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSM
Plasmid#67505PurposeExpress the tRNAMet scaffoldDepositorInsertmodified tRNAMet
UseTagsExpressionBacterialMutationPromoterlppAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKB223
Plasmid#170013PurposeEncodes LacI-controlled expression of surface-displayed C18G and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
C18G
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pALS3-Ma-sfGFP-WT
Plasmid#212119PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertsSUMO-sfGFP-WT
M. alvus Pyl-tRNA(6)
UseTagsHis6ExpressionBacterialMutationPromoteraraC and lppAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKB253
Plasmid#170036PurposeEncodes LacI-controlled expression of surface-displayed K5L7 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
K5L7
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceMay 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKB252
Plasmid#170035PurposeEncodes LacI-controlled expression of surface-displayed K6L9 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
K6L9
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB256
Plasmid#170039PurposeEncodes LacI-controlled expression of surface-displayed Bac2a and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Bac2a
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB254
Plasmid#170037PurposeEncodes LacI-controlled expression of surface-displayed H6L9 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
H6L9
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pBSM
Plasmid#67505PurposeExpress the tRNAMet scaffoldDepositorInsertmodified tRNAMet
UseTagsExpressionBacterialMutationPromoterlppAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKB232
Plasmid#170019PurposeEncodes LacI-controlled expression of surface-displayed murine CRAMP and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
CRAMP
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSK
Plasmid#67504PurposeExpress the tRNALys scaffoldDepositorInsertmodified tRNALys human
UseTagsExpressionBacterialMutationPromoterlppAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKB231
Plasmid#170018PurposeEncodes LacI-controlled expression of surface-displayed cecropin P1 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Cecropin P1
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAT112TAG-SepT
Plasmid#34624DepositorInserttRNA^Sep
UseTagsExpressionBacterialMutationPromoterlppAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only