Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 13,922 results for: SUB

Clear keywords
Showing: 51 - 100 of 13922 results

  6. B. subtilis Single Gene Deletion Library--Kanamycin

    Plasmid Kit
    #1000000115
    Purpose
    B. subtilis Single Gene Deletion Library - Kanamycin: Arrayed deletion library in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.
    Depositor
    Carol Gross
    Available Since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only

  7. B. subtilis CRISPRi Essential Gene Knockdown Library

    Plasmid Kit
    #1000000116
    Purpose
    B. subtilis CRISPRi Essential Gene Knockdown Library: Arrayed library that uses CRISPRi to sterically hinder transcription and therefore knock down expression of essential genes.
    Depositor
    Carol Gross
    Available Since
    Sept. 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  8. GABA(A) receptor subunit a1SE

    Plasmid
    #49168
    Purpose
    pHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Article
    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do
    Insert
    GABA(A) receptor subunit alpha-1 (Gabra1 Mouse)
    Tags
    myc and pHGFP (pHIuorin)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49168-plasmid-map-sequence-id-72484

    Enlarge

  9. GABA(A) receptor subunit g2SE

    Plasmid
    #49170
    Purpose
    pHluorin-tagged GABA A receptor subunit (gamma 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Article
    Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.
    Insert
    GABA(A) receptor subunit gamma 2 (Gabrg2 Mouse)
    Tags
    myc, pHGFP (pHluorin), and thrombin cleavage site…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49170-plasmid-map-sequence-id-165320

    Enlarge

  10. GABA(A) receptor subunit B3SE

    Plasmid
    #49171
    Purpose
    pHluorin-tagged GABA A receptor subunit (beta 3) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Article
    Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.
    Insert
    GABA(A) receptor subunit beta 3 (Gabrb3 Mouse)
    Tags
    myc, pHGFP (pHluorin), and thrombin cleavage site…
    Expression
    Mammalian
    Mutation
    GTG (V) to TGC (C)
    Promoter
    CMV
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49171-plasmid-map-sequence-id-72502

    Enlarge

  11. GABA(A) receptor subunit a2SE

    Plasmid
    #49169
    Purpose
    pHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Article
    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do
    Insert
    GABA(A) receptor subunit alpha-2 (GABRA2 Human)
    Tags
    HA, bovine alpha 1 signal sequence, and pHGFP (pH…
    Expression
    Mammalian
    Mutation
    T343A
    Promoter
    CMV
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49169-plasmid-map-sequence-id-72495

    Enlarge

  12. pIVTR(T7A)-NGN2 (SA Substitutions)

    Plasmid
    #172308
    Purpose
    In vitro transcription of NGN2 (Ser phosphosites substituted by Ala)
    Depositor
    Patrik Verstreken
    Insert
    NGN2
    Use
    Other
    Mutation
    S24A, S193A, S207A, S209A, S219A, S232A, S239A an…
    Promoter
    T7 promoter A-initiating
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172308-plasmid-map-sequence-id-340455

    Enlarge

  13. psubCAG-WPRE-hVEGF-D-Strept

    Plasmid
    #119227
    Purpose
    Plasmid encodes Strep-tagged full-length (unprocessed) VEGF-D of human origin to be used for stimulation of lymphangiogenesis in mice in vivo.
    Depositor
    Kari Alitalo
    Insert
    VEGFD (VEGFD Human)
    Use
    AAV
    Tags
    Strept
    Promoter
    CAG
    Available Since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    119227-plasmid-map-sequence-id-231678

    Enlarge

  14. pcDNA3-AMPKsub-YPet-NES

    Plasmid
    #84635
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub-YPet-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    84635-plasmid-map-sequence-id-176207

    Enlarge

  16. pcDNA3-AMPKsub(TA)-YPet-NES

    Plasmid
    #84636
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub(TA)-YPet-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available Since
    April 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    84636-plasmid-map-sequence-id-176880

    Enlarge

  17. pcDNA3-PKCsub-YPet-NES

    Plasmid
    #138207
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKCsub-YPet-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    138207-plasmid-map-sequence-id-307410

    Enlarge

  18. pIVTR(T7A)-ATOH1 (SA Substitutions)

    Plasmid
    #172309
    Purpose
    In vitro transcription of ATOH1 (Ser phosphosites substituted by Ala)
    Depositor
    Patrik Verstreken
    Insert
    ATOH1 (ATOH1 Human)
    Use
    Other
    Mutation
    S331A, S342A
    Promoter
    T7 promoter A-initiating
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172309-plasmid-map-sequence-id-340456

    Enlarge

  19. pcDNA3-JNKsubDD-YPet-NES

    Plasmid
    #84633
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    JNKsubDD-YPet-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    84633-plasmid-map-sequence-id-176152

    Enlarge

  20. psubCMV-mVEGF-D-dNdC-WPRE

    Plasmid
    #119228
    Purpose
    Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has native signal peptide to be used for stimulation of lymphangiogenesis in mice in vivo.
    Depositor
    Kari Alitalo
    Insert
    Vegfd-dN-dC (Vegfd Mouse)
    Use
    AAV
    Promoter
    CMV
    Available Since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    119228-plasmid-map-sequence-id-231686

    Enlarge

  21. pcDNA3-PKAsub-YPet-NES

    Plasmid
    #138203
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub-YPet-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    138203-plasmid-map-sequence-id-307404

    Enlarge

  22. pcDNA3-JNKsub(TA)DD-YPet-NES

    Plasmid
    #84634
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    JNKsub(TA)DD-YPet-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available Since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    84634-plasmid-map-sequence-id-176198

    Enlarge

  23. pcDNA3-ERKsub(TA)DD-Venus-NES

    Plasmid
    #84631
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    ERKsub(TA)DD-Venus-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Mutated target Thr residue to Ala.
    Promoter
    CMV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    84631-plasmid-map-sequence-id-175151

    Enlarge

  25. pcDNA3-PKCsub(T/A)-YPet-NES

    Plasmid
    #138208
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKCsub(T/A)-YPet-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available Since
    Sept. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    138208-plasmid-map-sequence-id-307411

    Enlarge

  26. pcDNA3-AMPKsub-YPet-Kras

    Plasmid
    #84638
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub-YPet-Kras
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    84638-plasmid-map-sequence-id-176882

    Enlarge

  27. pET41-FeoB-OL2/4sub

    Plasmid
    #216746
    Purpose
    Study the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL2 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Article
    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Outer loop 4 (OL4) with the sequence of [TLDGKPVQ…
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216746-plasmid-map-sequence-id-434435

    Enlarge

  28. pET41-FeoB-OL3/4sub

    Plasmid
    #216747
    Purpose
    Study the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL3 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Article
    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Outer loop 4 (OL4) with the sequence of [TLDGKPVQ…
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216747-plasmid-map-sequence-id-434432

    Enlarge

  29. pET41-FeoB-OL5/4sub

    Plasmid
    #216748
    Purpose
    Study the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL5 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Article
    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Outer loop 4 (OL4) with the sequence of [TLDGKPVQ…
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216748-plasmid-map-sequence-id-434431

    Enlarge

  31. pcDNA3-PKAsub(T/A)-YPet-NES

    Plasmid
    #138205
    Purpose
    Encodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub(T/A)-YPet-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138205-plasmid-map-sequence-id-307407

    Enlarge

  32. pcDNA3-PKAsub-YPet-Kras

    Plasmid
    #138206
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub-YPet-Kras
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138206-plasmid-map-sequence-id-307409

    Enlarge

  33. pcDNA3-PKAsub(T/A)-RLuc8N-NES

    Plasmid
    #138212
    Purpose
    Encodes negative-control substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub(T/A)-RLuc8N-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138212-plasmid-map-sequence-id-307414

    Enlarge

  34. PKAsub-TagRFPT-CAAX

    Plasmid
    #87712
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    PKAsub-TagRFPT-CAAX
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87712-plasmid-map-sequence-id-176157

    Enlarge

  35. PKAsub(TA)-TagRFPT-CAAX

    Plasmid
    #87740
    Purpose
    Negative-control PKA substrate fragment for bimFLINC-AKAR, targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    PKAsub(TA)-TagRFPT-CAAX
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87740-plasmid-map-sequence-id-176201

    Enlarge

  43. Human a4 nicotinic subunit crystallization construct

    Plasmid
    #85841
    Purpose
    pEZT-BM vector with alpha4 gene after CMV promoter, published crystallization construct
    Depositor
    Ryan Hibbs
    Article
    Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do
    Insert
    Human a4 nAChR subunit crystallization construct (CHRNA4 Human)
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85841-plasmid-map-sequence-id-171121

    Enlarge

  45. Human b2 nicotinic subunit crystallization construct

    Plasmid
    #85842
    Purpose
    pEZT-BM vector with beta2 gene after CMV promoter, published crystallization construct
    Depositor
    Ryan Hibbs
    Article
    Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do
    Insert
    Human b2 nAChR subunit crystallization construct (CHRNB2 Human)
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    C-terminal StrepII tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85842-plasmid-map-sequence-id-171117

    Enlarge

  47. Human a3 nicotinic receptor subunit EM construct

    Plasmid
    #163960
    Purpose
    pEZT-BM vector with alpha3 gene after CMV promoter, published EM construct
    Depositor
    Ryan Hibbs
    Article
    Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do
    Insert
    Human alpha3 nicotinic acetylcholine receptor subunit EM construct
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    apocytochrome b(562)RIL (BRIL)
    Expression
    Mammalian
    Mutation
    Asn348-Ser402 replaced with apocytochrome b(562)R…
    Promoter
    CMV
    Available Since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163960-plasmid-map-sequence-id-323882

    Enlarge

  50. Human b4 nicotinic receptor subunit EM construct

    Plasmid
    #163961
    Purpose
    pEZT-BM vector with beta4 gene after CMV promoter, published EM construct
    Depositor
    Ryan Hibbs
    Article
    Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do
    Insert
    Human beta4 nicotinic acetylcholine receptor subunit EM construct
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    StrepII tag and apocytochrome b(562)RIL (BRIL)
    Expression
    Mammalian
    Mutation
    Pro341-Ser395 replaced with apocytochrome b(562)R…
    Promoter
    CMV
    Available Since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163961-plasmid-map-sequence-id-323883

    Enlarge

Showing: 51 - 100 of 13922 results